Short communication: Metagenomic evaluation of skin biopsies of udder sores in dairy cows

Sorge, Ulrike S; Binger, Elizabeth M.; Schefers, Jeremy; Plummer, Paul J.

doi:10.3168/jds.2018-15863

Cited by 14 publications

(25 citation statements)

References 13 publications

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“…Our results show an altered microbiota in both mild and severe UCD lesions compared to the control samples, manifested by decreased bacterial diversity and an increased proportion of certain bacterial genera and species. In line with our results on diversity, Sorge et al [17] found a lower bacterial diversity in samples from UCD lesions compared to control samples. A similar finding was also reported in a study on digital dermatitis, in which bacterial diversity decreased as the digital dermatitis lesions progressed in severity [32].…”

Section: Discussionsupporting

confidence: 93%

“…have previously been identified in UCD lesions through culturing [3,13]. The genera Corynebacterium and Staphylococcus are also associated with bovine healthy skin from teat apices [38,39] and, in contrast to our results, Sorge et al [17] found a higher abundance of Corynebacterium spp. in controls than in UCD https://doi.org/10.1371/journal.pone.0242880.g008…”

Section: Plos Onecontrasting

confidence: 94%

“…In the fourth subgroup indicating dysbiosis, including several severe, but no mild, UCD samples, a more anaerobic microbiota was observed, including bacterial species such as Trueperella pyogenes, Fusobacterium necrophorum and Porphyromonas asaccharolytica. These bacteria have previously been identified in bacteriological studies of UCD [2,3] and, in line with our results, Sorge et al [17] found a higher abundance of these bacteria in UCD samples compared to control samples. Finding these opportunistic bacteria in severe UCD lesions is not surprising as they are associated with several bacterial conditions in ruminants, such as interdigital phlegmon, abscesses and wound infections [43,44].…”

Section: Plos Onesupporting

confidence: 92%

“…found in control samples is not known, but it could be speculated that these bacteria have a protective effect on the skin barrier. Sorge et al [17] also found Bifidobacterium spp. to be significantly more common in controls compared to UCD samples.…”

Section: Plos Onementioning

confidence: 91%

“…In recent decades, the use of culture-independent methods to identify the microorganisms present in a sample or an environment has become increasingly common [16]. So far, few studies have been performed on samples from UCD lesions, although a recent study used 16S rRNA-amplicon sequencing to investigate the bacterial microbiota of UCD lesions and compared it with that of healthy skin [17]. They found that certain bacterial genera were more common in samples from UCD lesions, such as Fusobacterium, Helcococcus, Anaerococcus, Trueperella and Porphyromonas, compared to samples from healthy skin.…”

Section: Introductionmentioning

confidence: 99%

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A shotgun metagenomic investigation of the microbiota of udder cleft dermatitis in comparison to healthy skin in dairy cows

et al. 2020

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Udder cleft dermatitis (UCD) is a skin condition affecting the fore udder attachment of dairy cows. UCD may be defined as mild (eczematous skin changes) or severe (open wounds, large skin changes). Our aims were to compare the microbiota of mild and severe UCD lesions with the microbiota of healthy skin from the fore udder attachment of control cows, and to investigate whether mastitis-causing pathogens are present in UCD lesions. Samples were obtained from cows in six dairy herds. In total, 36 UCD samples categorized as mild (n = 17) or severe (n = 19) and 13 control samples were sequenced using a shotgun metagenomic approach and the reads were taxonomically classified based on their k-mer content. The Wilcoxon rank sum test was used to compare the abundance of different taxa between different sample types, as well as to compare the bacterial diversity between samples. A high proportion of bacteria was seen in all samples. Control samples had a higher proportion of archaeal reads, whereas most samples had low proportions of fungi, protozoa and viruses. The bacterial microbiota differed between controls and mild and severe UCD samples in both composition and diversity. Subgroups of UCD samples were visible, characterized by increased proportion of one or a few bacterial genera or species, e.g. Corynebacterium, Staphylococcus, Brevibacterium luteolum, Trueperella pyogenes and Fusobacterium necrophorum. Bifidobacterium spp. were more common in controls compared to UCD samples. The bacterial diversity was higher in controls compared to UCD samples. Bacteria commonly associated with mastitis were uncommon. In conclusion, a dysbiosis of the microbiota of mild and severe UCD samples was seen, characterized by decreased diversity and an increased proportion of certain bacteria. There was no evidence of a specific pathogen causing UCD or that UCD lesions are important reservoirs for mastitis-causing bacteria.

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Section: Discussionsupporting

confidence: 93%

Section: Plos Onecontrasting

confidence: 94%

Section: Plos Onesupporting

confidence: 92%

Section: Plos Onementioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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A shotgun metagenomic investigation of the microbiota of udder cleft dermatitis in comparison to healthy skin in dairy cows

et al. 2020

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Prevalence and clinical relevance of udder thigh dermatitis in dairy cows

Sickinger

Avenarius

Wehrend

2022

Tierarztl Prax Ausg G Grosstiere Nutztiere

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Objective Udder thigh dermatitis (UTD) is especially common in heifers. Skin alterations and necrosis of muscle tissue characterise this condition and, although the disease is well understood, systematic analysis concerning prevalence, risk factors and consequences of UTD is lacking. The aim of this study, therefore were such systematic clinical examinations. Material and methods Statistical analyses of data were performed to objectively evaluate the supposed higher risk of heifers developing UTD. The study included a total of 317 animals that were examined and classified as UTD positive or negative on the day of calving. The size of skin alterations and the severity of tissue damage were assessed on days 7, 14 and 21 p. p. Results The overall prevalence resulted in 18 % UTD with a higher risk in heifers (36.8 %) than in cows (4.3 %; P < 0.0001). A significant link was found between the size of tissue lesions and severity of UTD (day 7: P = 0.007; day 14: P = 0.002; day 21: P = 0.011). Peripartal udder oedemas were strongly associated with UTD (P < 0.05). The milk yield and the concentration of milk protein was found to have decreased. Conclusion UTD appears to be a multi-factorial disease that is supposed to be painful due to the obvious avoidant behaviour in affected animals. In respect to the reduction in daily milk yield and an enhancement of pain related stress, neither the economic impact of UTD, nor the adverse effects on animal welfare should be underestimated. Clinical relevance Heifers with udder oedema are advised to be examined for the presence of UTD in order to start an early treatment and diminish disease related negative impacts.

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Spatial and temporal organization of jejunal microbiota in goats during animal development process

Zhang

Zhou

et al. 2020

J. Appl. Microbiol.

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Aims This research aimed to investigate the temporal bacterial colonization relating to non‐rumination, transition and rumination phases, together with the spatial organization of microbial community in the jejunal mucosa and digesta of goats. Methods and Results This study explored the colonization programme of the jejunal microbiota by employing 16S rRNA amplicon sequencing. The colonization pattern of jejunal bacterial community exhibited an age‐ and gut region‐dependent progression during animal development process. Approximately 268 bacterial signatures contributed to the discrimination between gut regions, with Lactobacillus, Ruminococcus, Eubacterium and Clostridium_sensu_stricto were enriched in the jejunal digesta, and Bacteroides and unclassified bacteria were enriched in the jejunal mucosa. Intriguingly, a shift from Lactobacillus to Butyrivibrio, Eubacterium and Ruminococcus after d 20 was observed for jejunal digesta. In mucosa, Bifidobacterium, Corynebacterium, Faecalibacterium and Roseburia increased with age (P < 0·05) while Arcobacter, Bacteroides and Porphyromonas peaked at d 10. Conclusions The jejunal bacterial community was settled after solid starter provision, which may mark the potential boundary of a timeframe for intervention in goats. The spatial heterogeneity highlighted the complicacy of ecological niches during manipulation of gut microbiota. Significance and Impact of the study The present study extended the understanding of microbial programming and niche specific in the jejunum among different life stages and the basal cognition of persistent enhancement of nutrient utilization and decline of enteric diseases in ruminants.

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Short communication: Metagenomic evaluation of skin biopsies of udder sores in dairy cows

Cited by 14 publications

References 13 publications

A shotgun metagenomic investigation of the microbiota of udder cleft dermatitis in comparison to healthy skin in dairy cows

A shotgun metagenomic investigation of the microbiota of udder cleft dermatitis in comparison to healthy skin in dairy cows

Prevalence and clinical relevance of udder thigh dermatitis in dairy cows

Spatial and temporal organization of jejunal microbiota in goats during animal development process

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