Short Communication Isolation and characterization of polymorphic microsatellite loci in Aleurodicus dispersus (Hemiptera, Aleyrodidae)

Abstract

ABSTRACT. Ten microsatellite markers were isolated and characterized from Aleurodicus dispersus, the spiraling whitefly, an exotic pest species that is considered to be one of the most serious agricultural pests on Hainan Island, China. The polymorphism of these loci was examined in individual whiteflies from Hainan Island and from the Canary Islands. All loci were polymorphic, with two to four alleles per locus. Mean observed and expected heterozygosity values were 0.773 and 0.585, respectively. These microsatellite markers provide powerful tools for ecological, epidemiological and population genetic studies on this highly invasive insect. Thirty insects were collected

Genetic Evidence for Diversity of Spiralling Whitefly,Aleurodicus dispersus(Hemiptera: Aleyrodidae) Populations in India

“…Similar results were reported by Ma et al (2011) who observed that microsatellite markers had heterozygosity from Hainan and Canary Island, which ranged from 0.100 to 0.933. Several studies have been carried out to characterize the extent of genetic diversity and differentiation of whitefly populations (Rekha et al 2005;De Barro 2005;Chu et al 2007;Tsagkarakou et al 2007).…”

“…SSR markers have been used to differentiate B. tabaci variants and to estimate the biotypes and genetic relationships of closely related populations from the same geographical location (De Barro et al 2003;Tsagkarakou et al 2007;Gauthier et al 2008). The primer sequences of around 10 SSR markers are publicly available for studying the A. dispersus genome (Ma et al 2011).…”

“…DNA samples from A. dispersus populations collected from 7 states of India were amplified using a set of 18 SSR primer pairs which included 7 SSR primers of A. dispersus (Ma et al 2011) and 11 SSR primers of B. tabaci (De Barro et al 2003;Tsagkarakou & Roditakis 2003;Gauthier et al 2008) as detailed in Table 2. Preliminary studies were done using SSR primers of B. tabaci to know the genetic variation among the A. dispersus populations.…”