Short communication: Intra- and inter-individual milk microbiota variability in healthy and infected water buffalo udder quarters

Catozzi, Carlotta; Cuscó, Anna; Lecchi, Cristina; Talenti, Andrea; Martucciello, Alessandra; Cappelli, Giovanna; Bonastre, Armand Sanchez; Francino, Olga; Ceciliani, Fabrizio

doi:10.3168/jds.2019-16352

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…This was detected in several individuals. Despite factors pertaining to the individual cow, such as immunity and genetics, that are known to influence the udder microbiota 37 , our results indicated that within-individual microbial variation is frequent. This is supported by the fact that certain taxa were often detected in only one quarter, rather than in all four quarters.…”

Section: Discussioncontrasting

confidence: 57%

A core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosis

Porcellato

Meisal

Bombelli

et al. 2020

Sci Rep

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The importance of the microbiome for bovine udder health is not well explored and most of the knowledge originates from research on mastitis. Better understanding of the microbial diversity inside the healthy udder of lactating cows might help to reduce mastitis, use of antibiotics and improve animal welfare. In this study, we investigated the microbial diversity of over 400 quarter milk samples from 60 cows sampled from two farms and on two different occasions during the same lactation period. Microbiota analysis was performed using amplicon sequencing of the 16S rRNA gene and over 1000 isolates were identified using MALDI-TOF MS. We detected a high abundance of two bacterial families, Corynebacteriaceae and Staphylococcaceae, which accounted for almost 50% of the udder microbiota of healthy cows and were detected in all the cow udders and in more than 98% of quarter milk samples. A strong negative correlation between these bacterial families was detected indicating a possible competition. The overall composition of the udder microbiota was highly diverse and significantly different between cows and between quarter milk samples from the same cow. Furthermore, we introduced a novel definition of a dysbiotic quarter at individual cow level, by analyzing the milk microbiota, and a high frequency of dysbiotic quarter samples were detected distributed among the farms and the samples. These results emphasize the importance of deepening the studies of the bovine udder microbiome to elucidate its role in udder health.

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Section: Discussioncontrasting

confidence: 57%

A core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosis

Porcellato

Meisal

Bombelli

et al. 2020

Sci Rep

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“…Our data suggests that part of the NASM we cultured may have originated from teat canal colonization rather than IMI. The absence of a difference in the proportion of culture positives between healthy and CMT-positive quarters may also be explained through the interdependence of microbiota among the quarters of an individual buffalo [ 41 ]. It has also been shown in dairy cows that an IMI in one single quarter may also evoke an inflammatory response in the other quarters of that animal [ 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Pathogen group-specific risk factors for intramammary infection in water buffalo

Singha,

Koop,

Rahman

et al. 2024

PLoS ONE

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A cross-sectional study was conducted to estimate the prevalence of intramammary infection (IMI) associated bacteria and to identify risk factors for pathogen group-specific IMI in water buffalo in Bangladesh. A California Mastitis Test (CMT) and bacteriological cultures were performed on 1,374 quarter milk samples collected from 763 water buffalo from 244 buffalo farms in nine districts in Bangladesh. Quarter, buffalo, and farm-related data were obtained through questionnaires and visual observations. A total of 618 quarter samples were found to be culture positive. Non-aureus staphylococci were the predominant IMI-associated bacterial species, and Staphylococcus (S.) chromogenes, S. hyicus, and S. epidermidis were the most common bacteria found. The proportion of non-aureus staphylococci or Mammaliicoccus sciuri (NASM), S. aureus, and other bacterial species identified in the buffalo quarter samples varied between buffalo farms. Therefore, different management practices, buffalo breeding factors, and nutrition were considered and further analyzed when estimating the IMI odds ratio (OR). The odds of IMI by any pathogen (OR: 1.8) or by NASM (OR: 2.2) was high in buffalo herds with poor milking hygiene. Poor cleanliness of the hind quarters had a high odds of IMI caused by any pathogen (OR: 2.0) or NASM (OR: 1.9). Twice daily milking (OR: 3.1) and farms with buffalo purchased from another herd (OR: 2.0) were associated with IMI by any pathogen. Asymmetrical udders were associated with IMI-caused by any bacteria (OR: 1.7). A poor body condition score showed higher odds of IMI by any pathogen (OR: 1.4) or by NASM (OR: 1.7). This study shows that the prevalence of IMI in water buffalo was high and varied between farms. In accordance with the literature, our data highlight that IMI can be partly controlled through better farm management, primarily by improving hygiene, milking management, breeding, and nutrition.

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“…Finally, samples were obtained from quarter milk. There is no information on the independence of each quarter from adjacent quarters in water buffalo, except for the microbiome 17 . In dairy cows, each quarter is anatomically independent and has its vascular system, nervous system, and suspensory apparatus, suggesting that they may be considered independent 52 , 53 , suggesting that they can be regarded as independent.…”

Section: Discussionmentioning

confidence: 99%

Changes in the lipidome of water buffalo milk during intramammary infection by non-aureus Staphylococci

Ceciliani

Audano

Addis

et al. 2022

Sci Rep

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This study aimed to determine the lipidome of water buffalo milk with intramammary infection (IMI) by non-aureus staphylococci (NAS), also defined as coagulase-negative staphylococci, using an untargeted lipidomic approach. Non-aureus Staphylococci are the most frequently isolated pathogens from dairy water buffalo milk during mastitis. A total of 17 milk samples from quarters affected by NAS-IMI were collected, and the lipidome was determined by liquid chromatography-quadrupole time-of-flight mass spectrometry. The results were compared with the lipidome determined on samples collected from 16 healthy quarters. The study identified 1934 different lipids, which were classified into 15 classes. The abundance of 72 lipids changed in NAS-IMI milk compared to healthy quarters. Significant changes occurred primarily in the class of free fatty acids. The results of this study provided first-time insight into the lipidome of dairy water buffalo milk. Moreover, the present findings provide evidence that NAS-IMI induces changes in water buffalo milk's lipidome.

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Short communication: Intra- and inter-individual milk microbiota variability in healthy and infected water buffalo udder quarters

Cited by 5 publications

References 27 publications

A core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosis

A core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosis

Pathogen group-specific risk factors for intramammary infection in water buffalo

Changes in the lipidome of water buffalo milk during intramammary infection by non-aureus Staphylococci

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