Short Communication Influence of Arg72Pro polymorphisms of TP53 on the response of buccal cells to radiotherapy

Pereira, Leandro de Mattos; Carvalho, Maria Raquel Santos; Fonseca, Carmen; Lima, Souza; Cerqueira, Eneida M. M.; Jorge, W.; Castro, Marcelo

doi:10.4238/2011.december.14.6

Cited by 5 publications

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“…A study by Schwartz et al in 2011 using the human lymphoblast cells revealed that cell lines with homozygous Proline coding allele were more likely to be resistant to radiation-induced chromatid break formation in G 2 chromosomal radiosensitivity assay compared to those containing two arginine coding alleles [36]. Another study by Pereira et al in 2011 revealed that the homozygous arginine coding alleles increase the number of cells with karyorrhexis in buccal cells of head and neck cancer patients after the first session of radiotherapy [37]. The effect of low dose and low dose rate of radiation is inconsistent and inconclusive [38].…”

Section: Association Between Tp53 Arg72pro Polymorphism and Radiosensmentioning

confidence: 99%

Assessment of Individual Radiosensitivity in Inhabitants of Takandeang Village － A High Background Radiation Area in Indonesia

et al. 2019

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People living in high background radiation areas (HBRAs) possibly develop the radioadaptive response (RAR) phenomenon. The Mamuju area in West Sulawesi Indonesia is known as an HBRA in Indonesia due to its high natural uranium contents. It is possible that RAR has developed in Mamuju inhabitants. To prove this hypothesis, here in this study, evaluation of the individual radiosensitivity in the inhabitants of Takandeang Village, Mamuju, was conducted using G 2 micronucleus (MN) assay. Association between blood groups and TP53 Arg72Pro polymorphism with individual radiosensitivity was also evaluated in this study. Using G 2 MN assay, we assessed the individual radiosensitivity of Takandeang Village inhabitants and control samples. For each sample, three parameters were calculated. The spontaneous (baseline) MN number, MN number after 0.5 Gy in vitro irradiation, and radiation-induced MN were calculated to predict the individual radiosensitivity. The radiation-induced MN was defined by subtracting the spontaneous MN number from the MN number after irradiation. The mean and SD of the number of micronuclei induced by radiation found in control group (CG) was set as the cutoff value to determine the individual radiosensitivity in all samples. The occurrence of a radiation-induced MN value higher than the mean CG + 1SD CG was scored as 1, indicating a milder radiosensitive phenotype, whereas a result higher than the mean CG + 2SD CG was scored as 2, and indicated a more severe radiosensitive phenotype. When the individual value was lower than the mean CG + 1SD CG, a score of 0 was attributed to the tested subject. The results showed that four individuals in Takandeang Village inhabitants had a milder radiosensitive phenotype, while the others were categorized as normal radiosensitive. A similar finding was also found in control samples. Our study failed to find any correlation between radiosensitivity and either blood group or the TP53 Arg72Pro polymorphism. Overall, our study revealed the possibility of RAR phenomena in Takandeang Village inhabitants. Further investigation using a different point of radiation dose value and larger sample number should be performed to validate this study results.Journal homepage: http://aij.batan.go.id

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Section: Association Between Tp53 Arg72pro Polymorphism and Radiosensmentioning

confidence: 99%

Assessment of Individual Radiosensitivity in Inhabitants of Takandeang Village － A High Background Radiation Area in Indonesia

et al. 2019

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“…Druzhinin et al (2015) reported that the frequencies of chromosomal aberrations in peripheral blood lymphocytes were significantly elevated in longterm resident children in a boarding school under con ditions of high exposure to radon. The polymorphism TP53 Arg72Pro (rs1042522) can affect the induction of cell apopto sis (Pereira et al, 2011) and the repair of damaged DNA (Hu et al, 2005). It is possible that the Arg72Pro genotype does not promote efficient DNA repair or apoptosis in comparison with Arg72Arg and these events lead to malignant transformation of cells.…”

Section: Discussionmentioning

confidence: 99%

Association of polymorphism <i>TP53</i> Arg72Pro with radon-induced lung cancer in the Kazakh population

et al. 2019

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Lung cancer is a problem of great concern and one of the commonest cancer diseases worldwide and in the Republic of Kazakhstan in particular. Radon exposure is classified as the second most important cause of lung cancer. According to the experts, the contribution of natural sources to the average annual radiation dose of the Kazakh population currently stands at 80 %, including 50 % from radon. However, the effect of radon on human health in the Republic of Kazakhstan is almost unknown. The tumor suppressor gene TP53 is a key mediator of the DNA damage response cascade following cell exposure to ionizing radiation. The common polymorphism TP53 Arg72Pro (rs1042522) is a risk factor for lung cancer in the Asian population, but until now no genetic association studies have been done in the Kazakh population. No information on the synergistic carcinogenic effect of radon exposure and polymorphism TP53 Arg72Pro (rs1042522) is available either. This paper presents the results of the study of association between alteration in the TP53 gene and radon-induced lung cancer risk in the Kazakh population. Genetic association was assessed in a case-control study including 44 radon-exposed patients with lung cancer, 41 patients with lung cancer without radon exposure and 42 age/sex-matched healthy controls. We found that polymorphism TP53 Arg72Pro (rs1042522) was associated with lung cancer risk in the Kazakh population (OR = 6.95, 95 % CI = 2.41–20.05). Individuals with the Arg72Pro genotype also showed a significantly higher risk of radon-induced lung cancer (OR = 8.6, 95 % CI = 2.6–28.59).

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“…Previous studies have shown that the p53 Pro72P subtype is more easily located in the nucleus, while the p53 Arg72R subtype is more easily located in the mitochondria under stress (22). Therefore, it is possible that the p53 Pro72p subtype may more effectively induce gene expression of nuclear DNA compared with the p53 Arg72R subtype (23). The polymorphism of the 72 codon of the p53 gene can affect the induction of cell apoptosis and the repair of damaged DNA, which are functions closely associated with the maintenance of the integrity of the nuclear envelope (23,24).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is possible that the p53 Pro72p subtype may more effectively induce gene expression of nuclear DNA compared with the p53 Arg72R subtype (23). The polymorphism of the 72 codon of the p53 gene can affect the induction of cell apoptosis and the repair of damaged DNA, which are functions closely associated with the maintenance of the integrity of the nuclear envelope (23,24). In addition, the polymorphism of the 72 codon of the p53 gene may affect the accumulation of mitochondrial DNA mutations by combining with DNA polymerase γ (19).…”

Section: Discussionmentioning

confidence: 99%

Association of TP53 codon 72 genotype polymorphism and environmental factors with esophageal squamous cell carcinoma in the Mongolian population of the Chinese region of Inner Mongolia

Wei

Wang

et al. 2017

Oncology Letters

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The present study analyzed the association of tumor protein p53 (TP53) Pro72Arg polymorphism with esophageal squamous cell carcinoma (ESCC) in the Mongolian population of Tongliao (Inner Mongolia, China). Restriction fragment length polymorphism-polymerase chain reaction was used to detect the genotype distribution of TP53 Pro72Arg polymorphism in 100 patients with ESCC and 50 healthy controls from the same population. Besides, the correlation between ESCC in Mongolian patients and various factors such as age, sex, cigarette smoking and alcohol consumption was analyzed. χ2 test revealed significant associations between alcohol consumption (P=0.00006) and cigarette smoking (P=0.00076) and ESCC in Mongolian patients. Notably, the Pro72 allele was significantly enriched in patients with ESCC compared with its abundance in the healthy control group, and the genotype of Pro/Arg on p53 codon 72 was confirmed to exhibit a significant correlation with ESCC in Mongolian patients. The present study demonstrated that alcohol drinking and cigarette smoking were risk factors for ESCC in the Mongolian population. Mongolian patients who carry the partocular genotype of Arg/Pro or Pro/Pro on p53 codon 72 may be more likely to develop ESCC. Compared with the p53 codon 72 genotype Arg/Arg, the TP53 Pro72 allele increased the risk of ESCC in Mongolian patients by 1.659-fold.

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Short Communication Influence of Arg72Pro polymorphisms of TP53 on the response of buccal cells to radiotherapy

Cited by 5 publications

References 0 publications

Assessment of Individual Radiosensitivity in Inhabitants of Takandeang Village － A High Background Radiation Area in Indonesia

Assessment of Individual Radiosensitivity in Inhabitants of Takandeang Village － A High Background Radiation Area in Indonesia

Association of polymorphism <i>TP53</i> Arg72Pro with radon-induced lung cancer in the Kazakh population

Association of TP53 codon 72 genotype polymorphism and environmental factors with esophageal squamous cell carcinoma in the Mongolian population of the Chinese region of Inner Mongolia

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