Short communication: Gut microbial colonization of the mouse colon using faecal transfer was equally effective when comparing rectal inoculation and oral inoculation based on 16S rRNA sequencing

Lützhøft, Ditte Olsen; Sánchez-Alcoholado, Lidia; Tougaard, Peter; Mentzel, Caroline M. Junker; Kot, Witold; Nielsen, Dennis Sandris; Hansen, Axel Kornerup

doi:10.1016/j.rvsc.2019.09.009

Cited by 6 publications

(3 citation statements)

References 36 publications

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“…It has been previously speculated that as oral gavage inoculum needs to pass through the acidic stomach environment, rectal administration may be more e cient 21 . However, a previous study of FMT in mice showed that speci c pathogen-free mice treated with antibiotics and then orally or rectally inoculated with donor mice gut microbiota had no differences in microbial community after inoculation 22 . As such, while rectal administration is preferable in e cacy and safety outcomes for clinical FMT use, oral gavage is often selected in mouse studies, presumably due to convenience 23 .…”

Section: Recipient Preparation and Fmt Administrationmentioning

confidence: 93%

Guidelines For Reporting On Animal Faecal Transplantation (GRAFT) Studies: Recommendations From A Systematic Review of Murine Transplantation Protocols

Secombe

Al-Qadami

Subramaniam

et al. 2021

Preprint

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Background/Objectives: Faecal microbiota transplant (FMT) is a powerful tool used to connect changes in gut microbial composition with a variety of disease states and pathologies. While FMT enables potential causal relationships to be identified, the experimental details reported in preclinical FMT protocols are highly inconsistent and/or incomplete. This limitation reflects a current lack of authoritative guidance on reporting standards which would facilitate replication efforts and ultimately reproducible science. We therefore systematically reviewed all FMT protocols used in mouse models with the goal of formulating recommendations on the reporting of preclinical FMT protocols. Methods: Search strategies were applied across three databases (PubMed, EMBASE and Ovid Medline) until June 30, 2020. Data related to donor attributes, stool collection, processing/storage, recipient preparation, administration and quality control were extracted.Results: A total of 1753 papers were identified, with 241 identified for data extraction and analysis. Of the papers included, 92.5% reported a positive outcome with FMT intervention. However, the vast majority of studies failed to address core methodological aspects including the use of anaerobic conditions (91.7% of papers lacked information), storage (49.4%), homogenisation (33.6%), concentration (31.5%), volume (19.9%) and administration route (5.3%). To address these reporting limitations, we developed the Guidelines for Reporting Animal Faecal Transplant (GRAFT) that guide reporting standards for preclinical FMT. Conclusion: The GRAFT recommendations will enable robust reporting of preclinical FMT design, and facilitate high quality peer review, improving the rigour and translation of knowledge gained through preclinical FMT studies.

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Section: Recipient Preparation and Fmt Administrationmentioning

confidence: 93%

Guidelines For Reporting On Animal Faecal Transplantation (GRAFT) Studies: Recommendations From A Systematic Review of Murine Transplantation Protocols

Secombe

Al-Qadami

Subramaniam

et al. 2021

Preprint

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“…One of the distinguishing features of IBS is the imbalance of intestinal flora, therefore reestablishing a healthy intestinal environment is of paramount importance to sufferers. FMT can control the intestinal state, enhance the barrier of the gastrointestinal mucosa, and aid in the reconstruction of the body's micro-ecosystem [9]. After therapy with FMT, the amount of lactobacilli in the feces increased from 5.5 CFU/g to 8.9 CFU/g and the number of bifidobacteria increased from 7.4 CFU/g to 8.9 CFU/g in a study including 42 individuals with IBS-D [10].…”

Section: Re-establishing the Intestinal Environmentmentioning

confidence: 99%

A comprehensive study on the efficacy of fecal microbiota transplantation in irritable bowel syndrome

Dai

Qin

2023

Second International Conference on Biological Engineering and Medical Science (ICBioMed 2022)

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Irritable Bowel Syndrome (IBS) is a physiological intestinal illness that is commonly accompanied with abdominal pain and changes in stool shape. Fecal Microbiota Transplantation (FMT) is a novel cure in intestinal diseases and an effective way to repopulate the gut flora. Paper will provide a comprehensive introduction to FMT and IBS, as well as a review of several studies on the value of FMT for IBS. It can be found in the studies that after short-term FMT treatment, patients' IBS-related symptoms were significantly improved, and only a few patients had minor intestinal problems such as diarrhea and constipation, and no serious intestinal disease. However, the effect of long-term treatment has not been further demonstrated, the IBS-related symptoms may recur in some patients, and the safety of long-term treatment has not been confirmed by experiments., this research examines the factors influencing FMT and the treatment's safety, as well as the potential clinical application of FMT for IBS. This will be an important topic for some time in the future, but it is certain that FMT will become one of the important treatment methods for IBS.

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“… 21 However, a previous study of FMT in mice showed that specific pathogen-free mice treated with antibiotics and then orally or rectally inoculated with donor mice gut microbiota had no differences in microbial community after inoculation. 22 As such, while rectal administration is preferable in efficacy and safety outcomes for clinical FMT use, oral gavage is often selected in mouse studies, presumably due to convenience. 23 In line with these findings, the overwhelming majority of studies included in our analysis opted for oral administration (90.4%; Figure 2g ) and only a small number used rectal administration (2.9%).…”

Section: Data Extractedmentioning

confidence: 99%

Guidelines for reporting on animal fecal transplantation (GRAFT) studies: recommendations from a systematic review of murine transplantation protocols

et al. 2021

View full text Add to dashboard Cite

Fecal microbiota transplant (FMT) is a powerful tool used to connect changes in gut microbial composition with a variety of disease states and pathologies. While FMT enables potential causal relationships to be identified, the experimental details reported in preclinical FMT protocols are highly inconsistent and/or incomplete. This limitation reflects a current lack of authoritative guidance on reporting standards that would facilitate replication efforts and ultimately reproducible science. We therefore systematically reviewed all FMT protocols used in mouse models with the goal of formulating recommendations on the reporting of preclinical FMT protocols. Search strategies were applied across three databases (PubMed, EMBASE, and Ovid Medline) until June 30, 2020. Data related to donor attributes, stool collection, processing/storage, recipient preparation, administration, and quality control were extracted. A total of 1753 papers were identified, with 241 identified for data extraction and analysis. Of the papers included, 92.5% reported a positive outcome with FMT intervention. However, the vast majority of studies failed to address core methodological aspects including the use of anaerobic conditions (91.7% of papers lacked information), storage (49.4%), homogenization (33.6%), concentration (31.5%), volume (19.9%) and administration route (5.3%). To address these reporting limitations, we developed the Guidelines for Reporting Animal Fecal Transplant (GRAFT) that guide reporting standards for preclinical FMT. The GRAFT recommendations will enable robust reporting of preclinical FMT design, and facilitate high-quality peer review, improving the rigor and translation of knowledge gained through preclinical FMT studies.

show abstract

Short communication: Gut microbial colonization of the mouse colon using faecal transfer was equally effective when comparing rectal inoculation and oral inoculation based on 16S rRNA sequencing

Cited by 6 publications

References 36 publications

Guidelines For Reporting On Animal Faecal Transplantation (GRAFT) Studies: Recommendations From A Systematic Review of Murine Transplantation Protocols

Guidelines For Reporting On Animal Faecal Transplantation (GRAFT) Studies: Recommendations From A Systematic Review of Murine Transplantation Protocols

A comprehensive study on the efficacy of fecal microbiota transplantation in irritable bowel syndrome

Guidelines for reporting on animal fecal transplantation (GRAFT) studies: recommendations from a systematic review of murine transplantation protocols

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