Short Communication: Erythrocytes assayed early ante mortem can predict adipose tissue and muscle<i>trans</i>-18:1 isomeric profiles of steers fed red clover silage supplemented with flaxseed

Mapiye, Cletos; DuganM., E. R.; TurnerT., D.; RollandD., C.; BasarabJ., A.; Baron, Vern S.; McAllister, Tim A.; BlockH., C.; UttaroB.,; AalhusJ., L.

doi:10.4141/cjas2012-054

Cited by 8 publications

(6 citation statements)

References 11 publications

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“…Previously we have observed large coefficients of variation in concentrations of PUFA BH products among cattle, especially for VA, when feeding FS in high-forage diets [7] , [16] , but the source of this inter-animal variation remains uncertain. Overall, differences in FA composition have been reported to originate from inter-animal variation in the rumen environment, including the kinds and numbers of rumen microbes present as well as other factors [17] .…”

Section: Introductionmentioning

confidence: 98%

Subcutaneous Adipose Fatty Acid Profiles and Related Rumen Bacterial Populations of Steers Fed Red Clover or Grass Hay Diets Containing Flax or Sunflower-Seed

et al. 2014

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Steers were fed 70∶30 forage∶concentrate diets for 205 days, with either grass hay (GH) or red clover silage (RC), and either sunflower-seed (SS) or flaxseed (FS), providing 5.4% oil in the diets. Compared to diets containing SS, FS diets had elevated (P<0.05) subcutaneous trans (t)-18:1 isomers, conjugated linoleic acids and n-6 polyunsaturated fatty acid (PUFA). Forage and oilseed type influenced total n-3 PUFA, especially α-linolenic acid (ALA) and total non-conjugated diene biohydrogenation (BH) in subcutaneous fat with proportions being greater (P<0.05) for FS or GH as compared to SS or RC. Of the 25 bacterial genera impacted by diet, 19 correlated with fatty acids (FA) profile. Clostridium were most abundant when levels of conjugated linolenic acids, and n-3 PUFA's were found to be the lowest in subcutaneous fat, suggestive of their role in BH. Anerophaga, Fibrobacter, Guggenheimella, Paludibacter and Pseudozobellia were more abundant in the rumen when the levels of VA in subcutaneous fat were low. This study clearly shows the impact of oilseeds and forage source on the deposition of subcutaneous FA in beef cattle. Significant correlations between rumen bacterial genera and the levels of specific FA in subcutaneous fat maybe indicative of their role in determining the FA profile of adipose tissue. However, despite numerous correlations, the dynamics of rumen bacteria in the BH of unsaturated fatty acid and synthesis of PUFA and FA tissue profiles require further experimentation to determine if these correlations are consistent over a range of diets of differing composition. Present results demonstrate that in order to achieve targeted FA profiles in beef, a multifactorial approach will be required that takes into consideration not only the PUFA profile of the diet, but also the non-oil fraction of the diet, type and level of feed processing, and the role of rumen microbes in the BH of unsaturated fatty acid.

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Section: Introductionmentioning

confidence: 98%

Subcutaneous Adipose Fatty Acid Profiles and Related Rumen Bacterial Populations of Steers Fed Red Clover or Grass Hay Diets Containing Flax or Sunflower-Seed

et al. 2014

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“…Mapiye et al. 9 have previously shown strong correlations between the VA content of erythrocytes and beef adipose tissue. Fatty acid analyses were conducted using gas chromatography, but this is expensive, time consuming, and demands a high level of technical expertise.…”

Section: Introductionmentioning

confidence: 93%

Using blood near infrared spectra from steers to classify fat and meat samples with low or high levels of vaccenic acid

et al. 2018

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Near infrared spectroscopy on steer blood was tested to discriminate between subcutaneous fat and longissimus thoracis samples with low or high levels of vaccenic acid. One day prior slaughter, blood samples from steers were harvested and near infrared spectra were collected on both whole blood and red blood cells. At slaughter, samples of subcutaneous fat and longissimus thoracis were collected and vaccenic acid content was analyzed by gas chromatography. Partial least squares discriminant analyses based on whole blood and red blood cell near infrared spectra were applied to classify subcutaneous fat and longissimus thoracis samples according to their content of vaccenic acid (low or high). Based on the results from the partial least squares discriminant analyses, subcutaneous fat and longissimus thoracis samples with low or high content of vaccenic acid could be discriminated from the blood near infrared spectra with accuracy from 74 to 95%. Application of near infrared spectroscopy technology on steer blood may have potential as early screening of live animals based on low or high levels of vaccenic acid in subcutaneous fat or longissimus thoracis.

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“…Development of methods to predict the in vivo abundance of trans ‐10 fatty acids in ruminant tissues would allow detection of animals more resistant to the trans ‐10 shift or with high proportions of trans ‐10 fatty acids in their tissues. Along this line, several studies have correlated t ‐18:1 composition of red blood cells (RBC) determined by GC‐FID with other tissues and found that most of t ‐18:1 isomers in RBC collected from cattle at slaughter were highly correlated with t ‐18:1 found in subcutaneous fat and muscle (Aldai, Dugan, et al, 2012; Mapiye et al, 2013). Recent studies also demonstrated that RBC are superior to plasma for predicting the t ‐18:1 composition in beef when feeding concentrate‐based diets (Moloney et al, 2021; Vahmani, Rolland, et al, 2020).…”

Section: Analysis Of Trans‐10 Intermediatesmentioning

confidence: 99%

Trans‐10 18:1 in ruminant meats: A review

Alves

Vahmani

Mapiye

et al. 2021

Lipids

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Trans (t) fatty acids (TFA) from partially hydrogenated vegetable oils (i.e., industrial trans) have been phased out of foods in many countries due to their promotion of cardiovascular disease. This leaves ruminant‐derived foods as the main source of TFA. Unlike industrial TFA where catalytic hydrogenation yields a broad distribution of isomers, ruminant TFA are enzymatically derived and can result in enrichment of specific isomers. Comparisons between industrial and ruminant TFA have often exonerated ruminant TFA due to their lack or at times positive effects on health. At extremes, however, ruminant‐sourced foods can have either high levels of t10‐ or t11‐18:1, and when considering enriched sources, t10‐18:1 has properties similar to industrial TFA, whereas t11‐18:1 can be converted to an isomer of conjugated linoleic acid (cis(c)9,t11‐conjugated linoleic acid), both of which have potential positive health effects. Increased t10‐18:1 in meat‐producing ruminants has not been associated with negative effects on live animal production or meat quality. As such, reducing t10‐18:1 has not been of immediate concern to ruminant meat producers, as there have been no economic consequences for its enrichment; nevertheless at high levels, it can compromise the nutritional quality of beef and lamb. In anticipation that regulations regarding TFA may focus more on t10‐18:1 in beef and lamb, the present review will cover its production, analysis, biological effects, strategies for manipulation, and regulatory policy.

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Short Communication: Erythrocytes assayed early ante mortem can predict adipose tissue and muscletrans-18:1 isomeric profiles of steers fed red clover silage supplemented with flaxseed

Cited by 8 publications

References 11 publications

Subcutaneous Adipose Fatty Acid Profiles and Related Rumen Bacterial Populations of Steers Fed Red Clover or Grass Hay Diets Containing Flax or Sunflower-Seed

Subcutaneous Adipose Fatty Acid Profiles and Related Rumen Bacterial Populations of Steers Fed Red Clover or Grass Hay Diets Containing Flax or Sunflower-Seed

Using blood near infrared spectra from steers to classify fat and meat samples with low or high levels of vaccenic acid

Trans‐10 18:1 in ruminant meats: A review

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