Short Communication

Mm, Mullally; Meisel, Hans; Rj, Fitzgerald

doi:10.1515/bchm3.1996.377.4.259

Cited by 69 publications

(1 citation statement)

References 24 publications

(16 reference statements)

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“…Thus, α s1 -CN-derived peptides RY, RYL, RYLG, YLGY and FYPEL exert both ACE-inhibitory and antioxidant activities [ 23 , 24 , 25 , 26 , 27 ]. ACE-inhibitory and opioid activities are exerted by sequences YGFLP [ 28 ] and YGFL [ 29 , 30 ]. Four of the analyzed peptides (RYLGY, LGY, YPFPGPI, and YLLF) have been reported to exert three or more activities.…”

Section: Resultsmentioning

confidence: 99%

In Silico and In Vitro Analysis of Multifunctionality of Animal Food-Derived Peptides

Amigo

Martínez-Maqueda

Hernández-Ledesma

2020

Foods

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Currently, the associations between oxidative stress, inflammation, hypertension, and metabolic disturbances and non-communicable diseases are very well known. Since these risk factors show a preventable character, the searching of food peptides acting against them has become a promising strategy for the design and development of new multifunctional foods or nutraceuticals. In the present study, an integrated approach combining an in silico study and in vitro assays was used to confirm the multifunctionality of milk and meat protein-derived peptides that were similar to or shared amino acids with previously described opioid peptides. By the in silico analysis, 15 of the 27 assayed peptides were found to exert two or more activities, with Angiotensin-converting enzyme (ACE) inhibitory, antioxidant, and opioid being the most commonly found. The in vitro study confirmed ACE-inhibitory and antioxidant activities in 15 and 26 of the 27 synthetic peptides, respectively. Four fragments, RYLGYLE, YLGYLE, YFYPEL, and YPWT, also demonstrated the ability to protect Caco-2 and macrophages RAW264.7 cells from the oxidative damage caused by chemicals. The multifunctionality of these peptides makes them promising agents against oxidative stress-associated diseases.

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