Short Communication: Effect of medium composition on the production by a new Bacillus subtilis isolate of protease with promising unhairing activity

Varela, Hermosinda; Ferrari, Mario Daniel; Belobrajdic, L; Weyrauch, R; Loperena, Lyliam

doi:10.1007/bf00327730

Cited by 47 publications

(23 citation statements)

References 2 publications

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“…It is well known fact that the metal ions in the fermentation medium greatly influence the protease production by microbes (Varela et al 1996). Results revealed that MnCl 2 stimulated the enzyme production (4091 U/gss), followed by FeSO 4 (3855 U/gss), whereas, CuSO 4, ZnSO 4 and CoCl 2 inhibited the production.…”

Section: Discussionmentioning

confidence: 93%

Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization

Santosh¹,

Bisht²,

Shikha³

et al. 2011

Afr. J. Biotechnol.

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The increase in agricultural practices has necessitated the judicious use of agricultural wastes into value added products. In this study, an extracellular, organic solvent and oxidant stable, serine protease was produced by Aspergillus flavus MTCC 9952 under solid state fermentation. Maximum protease yield was obtained when the strain was grown under wheat bran and corn cob mixture (1:1) incubated for 48 h at pH 9.0 and temperature 37°C with 50% of initial moisture content. The partially purified enzyme showed wide range of pH optima (8.0-12.0) and pH stability (7.0-12.0), whereas, optimum temperature was 40°C and was stable over a wide range of temperature 30-45°C. The protease was extremely stable towards several organic solvents. The enzyme retained 80% of its original activity in the presence of non ionic and ionic surfactants and 100% with 10% H 2 O 2 after 1 h of incubation at 30°C. In addition, the enzyme showed excellent compatibility with some commercial powder detergents. The compatibility of our protease with several detergents, oxidants and organic solvents suggests its possible use in detergent industry and peptide synthesis.

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Section: Discussionmentioning

confidence: 93%

Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization

Santosh¹,

Bisht²,

Shikha³

et al. 2011

Afr. J. Biotechnol.

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“…Maximum growth (2.14 x 10 11 CFU/ml) was observed in cultures using media M4 after 48 h of incubation. Protease synthesis is affected by the presence of easily metabolizable sugars and nitrogen sources in the medium Varela et al, 1996). Presently, no defined medium has been established for the optimum production of alkaline protease from different microbial sources.…”

Section: Effects Of Media Composition On Protease Productionmentioning

confidence: 99%

Nutritional factors affecting organic solvent-tolerant alkaline protease production by a newBacillus cereus strain 146

et al. 2006

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An organic solvent-tolerant bacterium designated as 146 capable of producing an organic solvent-stable alkaline protease was isolated from contaminated soil of a wood factory. The strain was a Gram-positive, spore-forming, nitrate-positive, rod-shaped organism capable of hydrolysing gelatine, starch, skim milk and identified as Bacillus cereus. Activity of the protease was drastically increased in the presence of 1-decanol, isooctane, n-dodecane and n-tetradecane, but reduced in the presence of ethyl acetate, benzene, toluene, 1-heptanol, ethylbenzene and hexane. The bacterium was shown to require lactose as a carbon source and peptone as a nitrogen source. The optimum fermentation condition for the production of alkaline protease was in the presence of beef and yeast extract. Optimum pH was determined to be at 10.0 at incubation temperature of 37 °C for 48 h. Results from the studies suggest that 146 is a new strain of Bacillus cereus capable of producing organic solvent-tolerant alkaline protease with potential use in industries.

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“…Therefore, the effect of various carbon and nitrogen nutrient cost-effective substrates, divalent metal ions, environmental and fermentation parameters such as pH, temperature, aeration, and agitation were evaluated in the literature (Adinarayana and Ellaiah, 2002;Varela et al, 1996). However, for optimization of all factors and establishment the best possible conditions by considering all interaction of parameters, numerous experiments have to be carried out, which is not economical and practical.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of alkaline protease production by Bacillus clausii using Taguchi experimental design

Oskouie¹,

Tabandeh²,

Yakhchali³

et al. 2007

Afr. J. Biotechnol.

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The effect of culture conditions on protease production and bacterial growth of Bacillus clausii was investigated using Taguchi design of experiment. Five factors viz., carbon source, organic and inorganic nitrogen sources, agitation and metal ion, each at four levels were selected and an orthogonal array layout of L16 (4 5 ) were performed. The proposed medium for alkaline protease production consisted of (g/l): sucrose, 10; yeast extract, 10; KNO 3 , 5; trace element without Mn 2+ . Under these optimal conditions, 4 fold enhancement in protease production (from 250 to 1000 U/ml) was obtained. At the optimum culture for bacterial growth, which contained (g/l): starch, 10; yeast extract, 10; ammonium ions, 5; trace element without Zn 2+ , 1.88 fold increase in growth production (from OD 600 nm of about 8.5 in basal medium to OD 600 nm of 16 in optimized medium) were observed. The inorganic nitrogen source was the most significant factor on protease production with 57.75% contribution. The organic nitrogen and carbon sources by 35.28 and 34.93% contributions were prominent factors in bacterial growth.

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Short Communication: Effect of medium composition on the production by a new Bacillus subtilis isolate of protease with promising unhairing activity

Abstract: In a search for microorganisms producing extracellular protease with unhairing activity, Bacillus subtilis IIQDB32 was isolated. Protease formation was significantly stimulated by glucose, tryptone, yeast extract, Ca(2+) and Mn(2+), but was repressed by ammonia and Fe(2+).

Cited by 47 publications

References 2 publications

Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization

Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization

Nutritional factors affecting organic solvent-tolerant alkaline protease production by a newBacillus cereus strain 146

Enhancement of alkaline protease production by Bacillus clausii using Taguchi experimental design

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