Short Communication: Dextran production using Leuconostoc mesenteroides strains isolated from Borassus flabellifer sap

Maunatin, Anik; Harijono, Harijono; Zubaidah, Elok; Rifa’i, Muhaimin

doi:10.13057/biodiv/d230260

Cited by 2 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to previous reports, different EPSs from Leu. mesenteroides were isolated with similar methods and characterized by SEM, including BI-20 EPS showing fibrous and branched structure ( Yilmaz et al, 2022b ), EPSs of N5 and N7 showing separately porous and tight structures ( Ma’unatin et al, 2022 ), S81 EPS possessing a sheet-like compact structure ( Taylan et al, 2019 ), and XR1 EPS exhibiting lotus leaf shape and compact structure ( Wang et al, 2021 ). Consequently, the honeycomb-like porous structure of RSG7 dextran has been rarely reported, which indicated the unique feature.…”

Section: Resultsmentioning

confidence: 99%

Structural characterization and partial properties of dextran produced by Leuconostoc mesenteroides RSG7 from pepino

Wang

Sun

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

Exopolysaccharides (EPSs) produced by lactic acid bacteria possess various bioactivities and potential attractions for scientific exploration and commercial development. An EPS-producing bacterial strain, RSG7, was previously isolated from the pepino and identified as Leuconostoc mesenteroides. Based on the analyses of high-performance size exclusion chromatography, high-performance ion chromatography, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and methylation, the RSG7 EPS was identified as a dextran with a molecular weight of 5.47 × 106 Da and consisted of α-(1→6) glycosidic linkages as backbone and α-(1→2), α-(1→3), α-(1→4), and α-(1→6) glycosidic linkages as side chains. Scanning electron microscopy observed a honeycomb-like porous structure of RSG7 dextran, and this dextran formed aggregations with irregular hill-shaped lumps according to atomic force microscopy analysis. Physical–chemical investigations suggested that RSG7 dextran possessed excellent viscosity at high concentration, low temperature, and high pH; showed a superior emulsifying capacity of tested vegetable oils than that of hydrocarbons; and owned the maximal flocculating activity (10.74 ± 0.23) and flocculating rate (93.46 ± 0.07%) in the suspended solid of activated carbon. In addition, the dextran could coagulate sucrose-supplemented milk and implied potential probiotics in vitro. Together, these results collectively describe a valuable dextran with unique characteristics for exploitation in food applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Structural characterization and partial properties of dextran produced by Leuconostoc mesenteroides RSG7 from pepino

Wang

Sun

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The surface of the EPS in this study was similar to the dextran produced by Leuc. pseudomesenteroides JF17 which was porous (Farinazzo et al 2019), Dextran N7 produced by Leuconostoc mesenteroides N7 exhibited a porous surface structure (Ma'unatin et al 2022). The potential of W. confusa isolated from longan (D. longan) fruit on EPS production has not been reported.…”

Section: Discussionmentioning

confidence: 99%

“…The surface morphology of EPS was observed by scanning electron microscopy. The EPS was fixed on an aluminum stub and gold-sputtered before SEM examination, maintaining an accelerated voltage of 10 kV (Farinazzo et al 2019;Ma'unatin et al 2022).…”

Section: Surface Morphology Of Eps With Scanning Electron Microscope ...mentioning

confidence: 99%

The Potential of Weissella confusa K2 Isolated from Longan (Dimocarpus longan) Fruit as High Exopolysaccharide-Producing Strain

Ma'unatin,

Khoirumiyah

2024

HAYATI J Biosci

View full text Add to dashboard Cite

Isolation of Lactic Acid Bacteria (LAB) from various sources has previously been carried out to obtain strains that produce high of exopolysaccharide (EPS). Isolate K2, which was LAB isolated from longan (Dimocarpus longan) fruit, can produce EPS. This study aimed to identify isolate K2, and evaluated the effect of supplementation carbon sources (glucose, fructose, sucrose, and lactose) and pH of media (4, 5, 6, 7, and 8) on EPS production. Isolate K2 was identified as Weissella confusa molecularly based on 16S rDNA, the type of carbon source, and the pH of media had significant effects (P<0.05) on EPS production. W. confusa K2 produced EPS that was highest on media with the supplementation of sucrose as a carbon source and produced the lowest EPS on lactose. The amount of EPS produced by W. confusa K2 under alkaline was higher than under acidic, the highest EPS of 47.703 g/L at pH 8 and the lowest EPS at pH 4 of 1.858 g/L. The physical properties showed EPS has good solubility in water with the solubility and Water Holding Capacity (WHC) at 40.533% and 354% respectively. Based on Surface morphology of EPS with Scanning Electron Microscope (SEM) analysis, the surface structure of EPS was a porous polymer matrix. Fourier Transform Infrared Spectrophotometer (FTIR) results of EPS showed the presence of absorption bands as characteristic of carbohydrates, such as the presence of O-H, C-H, C=O, C=C, C-O-C groups, and glycosidic bonds.

show abstract

Short Communication: Dextran production using Leuconostoc mesenteroides strains isolated from Borassus flabellifer sap

Cited by 2 publications

References 16 publications

Structural characterization and partial properties of dextran produced by Leuconostoc mesenteroides RSG7 from pepino

Structural characterization and partial properties of dextran produced by Leuconostoc mesenteroides RSG7 from pepino

The Potential of Weissella confusa K2 Isolated from Longan (Dimocarpus longan) Fruit as High Exopolysaccharide-Producing Strain

Contact Info

Product

Resources

About