Short Communication Development of universal primers for isolating fragments of the LEAFY gene

Abstract

ABSTRACT. LEAFY orthologs are found throughout land plants. Degenerate primers were designed for amplifying exon 3 region fragments of the LEAFY gene. Sixteen taxa from different plants were sampled, all of which were successfully isolated, which indicated that this set of primers is universally applicable for all land plants. With the use of this method, there may be potential for applying LEAFY exon 3 as a single-copy nuclear marker to resolve reticulate evolution at high taxonomic levels and to clarify the origin and evolutionary history of the LEAFY gene.

A digital PCR approach to assess the purity of oregano

“…Primers designed to amplify several candidates of nuclear single-copy genes for plants were retrieved from literature, such as TOPO6 [ 26 ] or LEAFY [ 27 ] but all our attempts to amplify plant material failed using these primers. Other potential candidates such as adh , actin , GAPDH have been shown to be prone to frequent gene duplication and partial recombination [ 28 , 29 ] and were therefore not considered.…”