Short Communication: Characterization of Rhizoctonia-like mycorrhizae associated with five Dendrobium species in Java, Indonesia

Abstract

Abstract. Soelistijono R, Utami DS, Daryanti, Faizin M, Dian R. 2020. Plankton biodiversity in various typologies of inundation in Paminggir swamp, South Kalimantan, Indonesia on dry season. Biodiversitas 21: 1007-1011. This study aims to determine the morphological and anatomical characteristics of Rhizoctonia-like mycorrhizae associated with the roots of five Dendrobium species; to determine the association between Rhizoctonia-like mycorrhizae with the root of five Dendrobium sp.; to obtain difference between Rhizoctonia-like mycorrhizae with the other in adjacent location Mycorrhizal observations of Rhizoctonia-like mycorrhizae in this study were carried out macroscopically (morphologically) and microscopically (anatomically). The macroscopic observation was performed by observing directly the development of fungal colonies on culture media. Microscopic observations were performed to determine the shape of the hyphal of fungi and the number of nuclei. The results showed that the Rhizoctonia-like mycorrhizae associated with the root of five species of Dendrobium sp. in Java were the binucleate Rhizoctonia groups (BNR). The binucleate Rhizoctonia has white colonies, right-angle branching hyphae, two nuclei, and brown hyphae. The association of the root of five species of Dendrobium sp.with Rhizoctonia-like mycorrhizae fungi was indicated by the existence of a peloton structure in cortical root tissue.

Diversity of EPIFIT Orchid Types in the Mangrove Ecosystem in Ayari Village, Teluk Ampimoi Sub District, Yapen Islands District, Papua Province, Indonesia

“…It is suspected that this is related to the morphological characters of the orchid in which the form of association shows a difference in mycorrhiza that is similar to Rhizoctonia and others in adjacent locations. Observations of mycorrhizae are similar to Rhizoctonia, especially the rough texture of the skin so that epiphytic orchids can easily attach their roots (Soelistijono et al 2020). In addition, the shape of the branches, the shape of the leaves that are wide enough and the crowns that are dense enough make this species able to create a microclimate that suits the needs of epiphytic orchids.…”

Rhizoctonia mycorrhizae application and watering intervals on Dendrobium violaceoflavens seedling: a study of its effect on drought stress

“…The research was carried out from July 2021 to May 2022. Rhizoctonia mychorrizae taken from the Larat orchid (Dendrobium phaleonopsis) collection of Soelistijono et al, (2020) in the tissue culture laboratory of Agriculture Faculty Universitas Tunas Pembangunan (UTP) Surakarta. The seedling D. violaceoflavens to be inoculated was obtained from tissue culture laboratory and inoculation was carried out in the experimental garden of UTP.…”

Important Role of Mycorrhiza for Seed Germination and Growth of <i>Dendrobium</i> Orchids

“…Rhizoctonia produced niacin that increased germination and growth (Soelistijono et al 2020). Rhizoctonia also increased seed germination up to 44-91% and advanced protocorm growth (Jiang et al 2015).…”

“…The plant will absorb the organic materials and peloton degraded. Hyphae degenerate after protocorm formation, usually in phases 4-5, leaving the empty hyphae (Chen et al 2014;Soelistijono et al 2020).…”