Short Communication: Bacillus endolithicus and Bacillus paramycoides: New isolates from housefly Musca domestica in Saudi Arabia

Abstract

Abstract. El-Ghwas DE, Al-Nasser AS, Al-Sheikhy AA. 2021. Short Communication: Bacillus endolithicus and Bacillus paramycoides: New Isolates from housefly Musca domestica in Saudi Arabia. Biodiversitas 22: 4209-4214. Housefly Musca domestica Linnaeus is a common insect widely distributed all over the world. It is one of the domestic insects found associated with humans and animals. The present study investigated the bacterial diversity associated with Musca domestica samples collected from different places such as food courts and trash cans near fast-food restaurants in Makkah Province from October 2019 to December 2019. Eighteen pure isolates of bacterial strains were isolated and identified by Gram staining. Most of the bacterial isolates were Gram-positive except for two species, which were Gram-negative. The VITEK system was used to identify randomly isolate no. 5, 7, 17, 18, and 29. The results revealed that they belonged to the genus Bacillus sp., Staphylococcus sp., Pseudomonas sp., and Micrococcus sp. respectively. Due to the most isolated strains were Bacillus sp., so 16S RNA was used to genetically identify novel isolated strains 5 and 7. Isolate no. 5 showed the highest similarity (99 %) with Bacillus endolithicus, and isolate no. 7 showed the highest similarity (99 %) with Bacillus paramycoides. This is the first record of Bacillus endolithicus and Bacillus paramycoides to be isolated from the house fly Musca domestica L.

Bacterial Species Associate on the Body Surface of Musca domestica L from Various Habitats based on 16S rRNA Sequencing

“…12 A recent study found over 100 bacterial pathogens, fungi, parasites, and viruses on or in M. domestica larvae and adults, some of which are potentially antimicrobial resistant. 13,14 Previous research was conducted, based on identification using 16S rRNA, several species of bacteria were found in M. domestica L. and Chrysomya megacephala L, namely Bifidobacterium, Alcaligenes faecalis, Brucella melitensis, Sphingobacterium spp., Gamma proteobacterium, and Enterobacter asburiae. 12 Research has been carried out to identify bacteria in various habitats isolated from the body surface of houseflies.…”

“…Forest Isolates (H.T.). 0.8% agarose gel Loaded DNA ladder per lane: 0.2 g Sample volume loaded per lane: 1µL for each 100 bp DNA Ladder (bp): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1500 100 bp DNA Ladder (ng/0.2ug):16,16,14,16, 34,12, 11,16,16, 20, 29 The DNA ladder cannot be used to compare the sizes of nonlinear DNA samples, such as plasmid DNA smallest was in the bacteria from the hospital (Table2).…”