Short Communication:  Assessment of cogongrass (Imperata cylindrica (L.) P.Beauv.) genetic variation in Java, Indonesia using atpB-rbcL and trnL-F intergenic spacer

Susanto, Agus Hery; Dwiati, Murni

doi:10.13057/biodiv/d230558

Cited by 4 publications

(5 citation statements)

References 37 publications

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“…This indicates very close genetic relationship among the five haplotypes, which can probably be caused by the presence of gene flow among the populations. Cogongrass seeds are easily spread by wind over distant areas [15].…”

Section: Resultsmentioning

confidence: 99%

Genetic Diversity of Cogongrass [Imperata Cylindrica (L.) Raeusch] in Java Island Based on psbA-trnH IGS

Dwiati¹,

Dewi²,

Susanto³

2023

Advances in Biological Sciences Research

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Cogongrass [Imperata cylindrica (L.) Raeusch] is a wild plant species widely distributed throughout various tropical and subtropical regions. The wide distribution may lead to genetic differences among the species populations, which can be analyzed using particular molecular markers showing high mutation rate. This study was aimed aims to assess the genetic diversity of cogongrass in Java Island, Indonesia, and the genetic relationship among the populations based on psbA-trnH IGS. Plant samples were taken randomly from several places in Java, namely Purwokerto, Jetis Beach, Purworejo, Yogyakarta, and Ponorogo. The molecular analysis involved genomic DNA extraction, amplification of psbA-trnH IGS, and sequencing of psbA-trnH IGS. The results showed that cogongrass populations in Java had a high genetic diversity with respect to haplotype diversity (h = 1) but low genetic diversity in terms of nucleotide diversity (π = 0.00658). This suggested a very close genetic relationship among cogongrass populations in the island, which can also be seen from the low values of genetic distances.

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Section: Resultsmentioning

confidence: 99%

Genetic Diversity of Cogongrass [Imperata Cylindrica (L.) Raeusch] in Java Island Based on psbA-trnH IGS

Dwiati¹,

Dewi²,

Susanto³

2023

Advances in Biological Sciences Research

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“…atpB-rbcL IGS (π = 0.0201) and trnL(UAA)-trnF(GAA) IGS (π = 0.2277). This was related to the ab-sence of diverse evolutionary origins, which might be due to the invasiveness of the species (Susanto & Dwiati, 2022). Invasive species are commonly subjected to a reduction in genetic diversity because of founder effects limiting their adaptability to new habitats.…”

Section: Resultsmentioning

confidence: 99%

“…It was reported that one individual plant could produce 31 rhizomes in ten months resulting in a total rhizome length of 12 m (Kato-Noguchi, 2022), although much lower rhizome germination was observed in cogongrass under continuously dark period in comparison to those in a light/dark regime (Hamidavi et al, 2021). High phenotypic plasticity and adaptability to nearly all terrestrial habitats also contribute wide distribution of cogongrass (Susanto & Dwiati, 2022).…”

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confidence: 99%

Molecular Profile of Cogongrass [Imperata cylindrica (L.) Raeusch] in Java Island Based on trnT(UGU)-trnL(UAA) IGS Sequences

Dwiati,

Rahmah,

Susanto

2023

Jurnal Biodjati

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Cogongrass is one of the top ten problem-causing weeds in the world that is invasively spread over many tropical and subtropical regions, including Java Island, the Republic of Indonesia. The wide distribution is possibly related to their adaptability to almost all terrestrial habitats from various altitudes, which may lead to a high level of genetic variation. This study aimed to assess the genetic variation and phylogenetic relationship of cogongrass in Java Island using an intergenic spacer (IGS) sequence in the cpDNA genome, i.e. trnT(UGU)-trnL(UAA), as the molecular marker of high mutation rate. Plant samples were collected randomly from five different sites on the island. These were subjected to total genomic DNA extraction. The DNAs obtained were then used as PCR templates to amplify trnT(UGU)-trnL(UAA) IGS using a pair of universal primers. The PCR products were sequenced for genetic analysis. It was shown that an extremely high genetic variation with respect to the haplotype diversity of cogongrass in Java Island was observed. Nevertheless, a very low nucleotide diversity was obtained revealing a considerably close genetic relationship among the samples. Anatomical data regarding stomata number and length were found to correspond to the high level of genetic variation, but it was not the case with the stomata index.

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“…9,13,17 Other sources for polyphenols are traditional herbal medicines. 12,[18][19][20] The root of Cogon grass (Imperata cylindrica (L.)), is a common traditional medicine in Indonesia, 21 has been proven to have antioxidant and anti-inflammatory properties due to its polyphenols contents. [22][23][24][25] Previous study found pretreatment of mice with oral cogon grass root extract (CGRE) for two weeks, significantly reduced serum cholesterol levels after acute olive oil loading.…”

Section: Research Articlementioning

confidence: 99%

Prebiotic Effect of Cogon Grass Root Extract on Stimulating Lactic Acid Bacteria Growth in Mice Intestine

Pradini,

Fauziah,

Asarina

et al. 2024

J. Med. Health

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Lactic acid bacteria (LAB) are known for their numerous beneficial effects for health and their colonization within intestines is affected by prebiotic consumption. Cogon grass root is an herbal medicine which contain substantial quantities of polyphenols. Recently, many studies suggested polyphenols as a novel group of prebiotics. Therefore, this study aimed to explore prebiotic effect of cogon grass root extract (CGRE) on stimulating growth of LAB in mice intestine. Mice (n =7/group) were divided into three groups; group A (control), group B and C received CGRE orally with daily dosage 90 and 115mg/kg BW, respectively. After 14 days of treatment, all mice were sacrificed. LAB was isolated and counted from ileum. Additional biochemical tests were performed to confirm the bacteria as LAB. Further identification of representatives LAB was performed with API-CHL-50 System. We found increased growth and diversity of LAB in the treatment group with optimum growth stimulation at CGRE dosage 90mg/KgBW daily (group B). Further identification showed Lactococcus lactis was the most frequent LAB found in all groups, with additional Lactobacillus paracasei and Lactobacillus curvatus were found in the treatment group. These findings support additional benefit of CGRE as prebiotics by stimulating LAB growth and diversity in mice intestine.

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Short Communication: Assessment of cogongrass (Imperata cylindrica (L.) P.Beauv.) genetic variation in Java, Indonesia using atpB-rbcL and trnL-F intergenic spacer

Cited by 4 publications

References 37 publications

Genetic Diversity of Cogongrass [Imperata Cylindrica (L.) Raeusch] in Java Island Based on psbA-trnH IGS

Genetic Diversity of Cogongrass [Imperata Cylindrica (L.) Raeusch] in Java Island Based on psbA-trnH IGS

Molecular Profile of Cogongrass [Imperata cylindrica (L.) Raeusch] in Java Island Based on trnT(UGU)-trnL(UAA) IGS Sequences

Prebiotic Effect of Cogon Grass Root Extract on Stimulating Lactic Acid Bacteria Growth in Mice Intestine

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