Short Communication: Analysis of purity and concentration of DNA extracted from intron patho gene-spin extraction on crab processed food product samples

Abstract

Abstract. Sophian A, Purwaningsih R, Muindar, Igirisa EPJ, Amirullah ML. 2021. Short Communication: Analysis of purity and concentration of DNA extracted from intron patho gene-spin extraction on crab processed food product samples. Asian J Trop Biotechnol 18: 28-31. The purpose of this study was to optimize the Intron patho gene-spin DNA/RNA extraction kit on crab processed food products so that it can be used for testing samples other than viral and bacterial samples. The method used in purity and concentration analysis to analyze total DNA (yield) is the absorbance method using a nanophotometer and the method used to isolate the sample DNA is the centrifuge Column or spin column method. The research data showed that the extracted sample concentration was in the range of 15.15-15.75, with an average of 15.46. while the purity values measured at the A260/A280 wavelengths were obtained with a purity range between 2,124-2,346. The total DNA analysis (yield) results obtained an average of 755.04 with the lowest yield value of 575.50 and the highest yield value of 787.50. As a suggestion for further research, it is hoped that modification can be made to perfect the isolated DNA so that it has better purity and concentration values.

The effect of gamma ray re-irradiation on genetic variations in black rice based on RAPD and Bph gene resistance location based on SSR markers

“…However, according to Abdel-Latif and Osman (2017); Aboul-Maaty and Oraby (2019) [29][30], the purity of DNA is still included in a good purity coefficient, in the range of >1.5 and <1.9 or in the range of 1.8. DNA purity value between 1.8-2.0 is also included in high-quality DNA purity [31]. The concentration of DNA extracted from this research showed a range between 116-394 ug/ml.…”

Analysis of the Quality of Isolated DNA in the Making of Guinea Pig DNA Test Standards

“…This is because enzymes work quite effectively by directly targeting amino acid bonds in protein lysis. The proteinase K enzyme is active at a temperature of 65-70°C; thus, in some studies using this method, it is sometimes necessary to optimize the process before applying it (Utaminingsih et al, 2022;Sophian, et al, 2021b;Sophian, et al, 2021a;Sutanta et al, 2021). The purpose of this heating process is to activate the proteinase K enzyme so that it can efficiently perform lysis (Renshaw et al, 2015).…”

“…Research conducted by Sophian & Syukur (2021) shows that good-quality DNA isolation can be achieved from the meat of the target species. During DNA isolation, the main parameters that must be fulfilled are the concentration and purity values of the isolated DNA (Sophian, 2021c;Sophian, Purwaningsih, et al, 2021a;Utaminingsih et al, 2022) Based on the background above, this research was conducted to examine the quality of DNA isolated during the production of guinea pig DNA test standards. The purpose of this research is to offer an alternative reference for the manufacturing of species-specific DNA standards.…”

The yield and purity of DNA extracts from seeds of eight six accessions of Treculia species using Zymo Research mini-prep DNA extraction kit

“…Low, moderate to high values of DNA yield were observed. A very large value of 2089.4 observed was considered by Alfi (2021) to be a very good yield. Low values of 6.1 ng/µl, 42.2 ng/µl, and 45.7 ng/µl from accessions Ab6, Ab4, and C46 respectively were similar to values (24.2 ng/µl, 43.11 ng/µl, 25.33 ng/µl, 27.25 ng/µl, and 36.18 ng/µl) observed in five tomato cultivars extracted with ZR kit method (Ganiyu et al, 2017).…”