Short communication: A potential role of tannins in the control of American foulbrood

Martínez, Pablo Giménez; Maggi, Matías; Fuselli, Sandra R.

doi:10.5424/sjar/2021193-17628

Cited by 1 publication

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The in vitro results showed that the most effective treatment was the cinnamon oil. Furthermore, Giménez et al (2021) found that among thirteen tested natural molecules, menadione, lauric acid, monoglyceride of lauric acid and naringenin showed antimicrobial activity against ten P. larvae isolates. This study demonstrated the in vitro bactericidal activity of these molecules, specifically menadione and lauric acid, at concentrations practical for field application.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the antibacterial activity of inflorescences and roots extracts of Cannabis ( Cannabis sativa L) against P. larvae has been reported ( Giselle et al, 2023 ). Researchers have also reported tannin extract to be potent antimicrobials against P. larvae ( Giménez et al, 2021 ). The extract of Dicranum polysetum has also shown a significant reduction in infection among honey bee larvae, with clinical cessation of infection upon extract administration within the initial 24 h after spore ingestion.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Core proteome mediated subtractive approach for the identification of potential therapeutic drug target against the honeybee pathogen Paenibacillus larvae

Rebhi,

Basharat,

Wei

et al. 2024

PeerJ

View full text Add to dashboard Cite

Background & Objectives American foulbrood (AFB), caused by the highly virulent, spore-forming bacterium Paenibacillus larvae, poses a significant threat to honey bee brood. The widespread use of antibiotics not only fails to effectively combat the disease but also raises concerns regarding honey safety. The current computational study was attempted to identify a novel therapeutic drug target against P. larvae, a causative agent of American foulbrood disease in honey bee. Methods We investigated effective novel drug targets through a comprehensive in silico pan-proteome and hierarchal subtractive sequence analysis. In total, 14 strains of P. larvae genomes were used to identify core genes. Subsequently, the core proteome was systematically narrowed down to a single protein predicted as the potential drug target. Alphafold software was then employed to predict the 3D structure of the potential drug target. Structural docking was carried out between a library of phytochemicals derived from traditional Chinese flora (n > 36,000) and the potential receptor using Autodock tool 1.5.6. Finally, molecular dynamics (MD) simulation study was conducted using GROMACS to assess the stability of the best-docked ligand. Results Proteome mining led to the identification of Ketoacyl-ACP synthase III as a highly promising therapeutic target, making it a prime candidate for inhibitor screening. The subsequent virtual screening and MD simulation analyses further affirmed the selection of ZINC95910054 as a potent inhibitor, with the lowest binding energy. This finding presents significant promise in the battle against P. larvae. Conclusions Computer aided drug design provides a novel approach for managing American foulbrood in honey bee populations, potentially mitigating its detrimental effects on both bee colonies and the honey industry.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%