Short‐circuit current and total conductance measurements on rabbit ileum.

Jesus, C. Henriques de

doi:10.1113/jphysiol.1977.sp011861

Cited by 2 publications

(3 citation statements)

References 11 publications

(13 reference statements)

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“…This result is different from the findings in rabbit ileum (Henriques de Jesus, 1977), where the additions of 2-deoxy-D-glucose caused a decrease in short circuit current and an increase in total conductance, these being linearly correlated. This was interpreted as meaning that the change in the electrical parameters was associated with an alteration of a resistive element in the electrical model.…”

Section: Discussioncontrasting

confidence: 56%

“…Previous studies on new-born pig ileum and rabbit ileum (Henriques de Jesus, , 1977 have shown that the increase in short circuit current caused by adding glucose to the mucosal side of the preparation is accompanied by an increase in the total conductance in such a way that the relationship between both variables follows a straight line with a positive slope. A different relationship was found for another sugar molecule, 2-deoxy-D-glucose, which causes a decrease in short circuit current and an increase in total conductance.…”

Section: Introductionmentioning

confidence: 99%

“…10 cm was dissected and divided into two parts which were mounted in paired chambers. These chambers have been described previously (Henriques de Jesus, 1977) and were designed to minimize edge damage and to keep good conditions of oxygenation and temperature of the preparations. The half-chambers were filled with Krebs phosphate solution (5 ml.)…”

Section: Methodsmentioning

confidence: 99%

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Short circuit current and total conductance measurements on rat ileum

Emilio¹,

Jesus²

1977

The Journal of Physiology

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SUMMARY1. Short circuit current and total conductance measurements were made on preparations of rat terminal ileum, using either the intact tissue or the isolated epithelium.2. The effects of D-glucose, 3-0-methyl-D-glucose, and 3-deoxy-3-fluor-D-glucose, added in suitable concentrations to the external bathing medium, were studied.3. An increase in both short circuit current and total conductance was detected when these sugars were used. It was possible to determine a linear relationship between the two variables and to study the kinetics of the interaction of glucose and 3-0-methyl-D-glucose with the sodium sugar carrier by using increasing concentrations of these substances.4. The increases in short circuit current and total conductance were also verified in isolated epithelium.5. Phlorizin, or the withdrawal of glucose, reversed the increase in short circuit current, but the total conductance remained unchanged.6. Increasing concentrations of 2-deoxy-D-glucose added to the mucosal bathing medium caused a decrease in short circuit current without changing the total conductance, either in preparations of intact ileum or in isolated epithelium.7. The meaning of these variations in the electrical parameters of the preparations is discussed.

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Section: Discussioncontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Short circuit current and total conductance measurements on rat ileum

Emilio¹,

Jesus²

1977

The Journal of Physiology

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Prinzipien des epithelialen Transportes in Niere und Darm

1979

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Epithelia of kidney and small intestine consist of one layer of cells which, at their luminal edge, are linked together by terminal bars. Solute transport proceeds either across the cells, which is true of all active transports, or it proceeds paracellularly through the basolateral spaces and terminal bars and is then passive. The driving force for the active transport of a substance is derived either directly from metabolism (primary active transport), or from the gradient of another solute, usually Na+, which in turn is created by primary active transport. In the latter case the transport is referred to as secondary active. The driving forces of passive transport are the electrochemical gradient of the respective substance and solvent drag. The proximal parts of the kidney as well as of the intestine are leaky so that a considerable part of net reabsorption proceeds passively. Their distal parts, however, where the transport is regulated, are tight so that large concentration differences can be created and maintained. Transcellular active transport is only possible if the cells are polar, i.e., the transport characteristics of the luminal cell membrane differ from those of the contraluminal cell membrane. By measuring the cellular electrical potential difference or by measuring transport into isolated plasma membrane vesicles from either cell side the driving forces for the two transport steps, the luminal and contraluminal, have been elucidated. Schemes for the transport steps in the proximal tubule and in the small intestine are given. They show the principal similarity of the transport of many substances in both epithelia.

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Short‐circuit current and total conductance measurements on rabbit ileum.

Abstract: SUMMARY1. Short-circuit current (SCC) and total conductance (at) measurements were made in in vitro preparations of rabbit terminal ileum.

Cited by 2 publications

References 11 publications

Short circuit current and total conductance measurements on rat ileum

Short circuit current and total conductance measurements on rat ileum

Prinzipien des epithelialen Transportes in Niere und Darm

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