Short-chain fatty acids enhance diffusional Ca transport in the epithelium of the rat cecum and colon

Mineo, Hitoshi; Hara, Hajime; Tomita, Fusao

doi:10.1016/s0024-3205(01)01146-8

Cited by 64 publications

(48 citation statements)

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“…A reduction in TEER across Caco-2 cell monolayers is considered to reflect an effect on tight junction-mediated paracellullar ion permeability (McRoberts et al 1990). While short-chain fatty acids, such as acetate, propionate and butyrate, have recently been shown to stimulate paracellular Ca transport individually in vitro in the epithelium of the rat caecum and colon (Mineo et al 2001), this is the first report, to our knowledge, which shows that mediumchain fatty acids, as well as a specific isomer of CLA (trans-10,cis-12 CLA), can modulate paracellular Ca transport across intestinal epithelial cells. The increase in paracellular Ca transport brought about by exposure to these fatty acids, however, did not translate into a significantly increased total transepithelial Ca transport across the Caco-2 monolayer.…”

Section: Discussionmentioning

confidence: 99%

The effect of conjugated linoleic acid and medium-chain fatty acids on transepithelial calcium transport in human intestinal-like Caco-2 cells

Jewell

Cashman

2003

Br J Nutr

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Capric (10 : 0) and lauric (12 : 0) acid and conjugated linoleic acid (CLA) have been shown to increase paracellular permeability across human intestinal-like Caco-2 cell monolayers. While this has generated interest in terms of improved drug absorption and delivery, little has been done in terms of their potential effect on nutrient transport across the intestinal epithelium. Therefore, the objective of the present study was to investigate the effect of these fatty acids on transepithelial Ca transport in Caco-2 cells. Cells were seeded onto permeable transport membranes and allowed to differentiate, over 21 d, into intestinal-like cell monolayers. Monolayers (n 9 per treatment) were exposed to 0 (control) or 80 mM-10 : 0, 80 mM-12 : 0, 80 mM-18 : 2, 80 mM-CLA (mixed isomers), 80 mM-cis-9,trans-11 CLA or 80 mM-trans-10,cis-12 CLA for 22 d after seeding (chronic effect) or for 24 h before Ca transport studies (acute effect) on day 22. After exposure, transepithelial and transcellular transport of 45 Ca, fluorescein transport (a marker of paracellular Ca transport) and transepithelial electrical resistance (TEER, an indicator of permeability) were measured. Overall Ca transport and TEER in Caco-2 cells were unaffected by exposure to any of the fatty acids for 24 h, or to 18 : 2, CLA blend or cis-9,trans-11 CLA for 22 d. Paracellular (but not total transepithelial and transcellular) Ca transport across Caco-2 cells was significantly increased (P,0·01, by about 1·5-fold relative to the control value) by exposure to 10 : 0, 12 : 0 and trans-10,cis-12 CLA for 22 d, suggesting that these nonesterified fatty acids could potentially enhance Ca absorption in vivo.

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Section: Discussionmentioning

confidence: 99%

The effect of conjugated linoleic acid and medium-chain fatty acids on transepithelial calcium transport in human intestinal-like Caco-2 cells

Jewell

Cashman

2003

Br J Nutr

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“…It has been reported that acidification in the ileal lumen with lactulose intake increases the apparent Ca and Mg absorptions (8). It has also been reported, in an experiment using the Ussing-chamber technique, that short-chain fatty acids directly increase the Ca permeability of the intestinal mucosa (27). Fermentative degradation of indigestible oligosaccharides such as lactulose by intestinal bacteria produces hydrogen gas, which is absorbed in the intestine and excreted via expiration.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lactulose on Calcium and Magnesium Absorption: A Study Using Stable Isotopes in Adult Men

Seki

Hamano

Iiyama

et al. 2007

J Nutr Sci Vitaminol

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SummaryTo evaluate the effect of lactulose on calcium (Ca) and magnesium (Mg) absorption, we performed a clinical trial with a double-blind, randomized, crossover design in 24 healthy adult male volunteers. The absorptions of Ca and Mg were evaluated by a single-labeling method using stable isotopes. The test foods, containing lactulose at a dose of 0 g (placebo), 2 g (low-dose), or 4 g (high-dose) together with 300 mg of Ca containing 20 mg of Mg) increased with lactulose dosage. Significant differences were observed in the Ca ratio between placebo and high-dose lactulose ( p Ͻ 0.01), and in the Mg ratio between placebo and low-dose lactulose and between placebo and high-dose lactulose ( p Ͻ 0.01). Lactulose ingestion did not change the levels of bone-resorption markers (type I collagen cross-linked N -telopeptide and deoxypyridinoline) in urine. The test foods did not cause any side effects. This study demonstrates that lactulose enhances the absorptions of Ca and Mg in adult men.

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“…DFAIII is known to be involved in promoting calcium absorption in the large intestine [10,[12][13][14]. Therefore, further research needs to be done in the large intestine on this topic.…”

Section: Main Effects Interactionsmentioning

confidence: 99%

“…Fax: +81-45-820-3526 E-mail: hachiya@fancl.co.jp DFAIII is fermented in the large intestine, producing shortchain fatty acids that promote calcium absorption [10,14]. The promoting effect of DFAIII on calcium absorption has been demonstrated by in vivo balance studies and human urinary calcium excretion [15] and balance studies.…”

Section: Introductionmentioning

confidence: 99%

Difructose Anhydride III Enhances Zinc Absorption in vivo: Zinc Gluconate is More Suitable for the Effects of Difructose Anhydride III than Zinc Yeast

Hachiya

Okuhara

Raneva

et al. 2006

J. Clin. Biochem. Nutr.

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Summary We examined the effect of difructose anhydride III (DFAIII) on zinc absorption in rats in two experiments. First, we compared the absorption of zinc from zinc gluconate and zinc yeast with or without DFAIII by measuring concentrations in plasma collected from the portal vein after a single oral administration of zinc gluconate or zinc yeast with or without DFAIII. The peak plasma zinc concentration and the incremental area under the curve (IAUC) of plasma zinc 6 h after administration were highest in the zinc gluconate with DFAIII group. Second, we examined the effect of DFAIII on the absorption of zinc with phytic acid. with or without DFAIII. Plasma zinc levels in groups both with and without DFAIII were nearly the same as those at the baseline. Our results showed that DFAIII was better with zinc gluconate than zinc yeast for zinc absorption, and that DFAIII does not alter the inhibitory effects of phytic acid on zinc absorption.

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Short-chain fatty acids enhance diffusional Ca transport in the epithelium of the rat cecum and colon

Cited by 64 publications

References 0 publications

The effect of conjugated linoleic acid and medium-chain fatty acids on transepithelial calcium transport in human intestinal-like Caco-2 cells

The effect of conjugated linoleic acid and medium-chain fatty acids on transepithelial calcium transport in human intestinal-like Caco-2 cells

Effect of Lactulose on Calcium and Magnesium Absorption: A Study Using Stable Isotopes in Adult Men

Difructose Anhydride III Enhances Zinc Absorption in vivo: Zinc Gluconate is More Suitable for the Effects of Difructose Anhydride III than Zinc Yeast

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