Short- and long-term low-salinity acclimation effects on the branchial and intestinal gene expression in the European seabass (Dicentrarchus labrax)

Kokou, Fotini; Con, Pazit; Barki, Assaf; Nitzan, Tali; Slosman, Tatiana; Mizrahi, Itzhak; Cnaani, Avner

doi:10.1016/j.cbpa.2019.01.018

Cited by 33 publications

(26 citation statements)

References 41 publications

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“…PepT1b protein expression on the other hand, started only in the middle intestine. Until now, intestinal co-expression of these two variants were only reported in adult tilapia (Huang et al, 2015; Con et al, 2017; Chourasia et al, 2018), Asian weather loach ( Misgurnus anguillicaudatus ) (Gonçalves et al, 2007), killifish ( Fundulus heteroclitus ) (Bucking and Schulte, 2012), and European sea bass (Kokou et al, 2019). These two variants, found in numerous fish species, resulted from the teleost-specific whole-genome duplication (Gonçalves et al, 2007; Con et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, there has been increased interest and research on PepTs’ participation in protein absorption in fish under different environmental and dietary conditions (Hakim et al, 2009; Bakke et al, 2010b; Bucking and Schulte, 2012; Koven and Schulte, 2012; Verri et al, 2016; Con et al, 2017; Orozco et al, 2017; Chourasia et al, 2018; Hallali et al, 2018; Kokou et al, 2019). However, there are few studies addressing these transporters at the larval and juvenile stages.…”

Section: Introductionmentioning

confidence: 99%

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Peptide Transporters in the Primary Gastrointestinal Tract of Pre-Feeding Mozambique Tilapia Larva

et al. 2019

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Fish larvae differ greatly from the adult form in their morphology and organ functionality. The functionality of the gastrointestinal tract depends on the expression of various pumps, transporters, and channels responsible for feed digestion and nutrients absorption. During the larval period, the gastrointestinal tract develops from a simple closed tube, into its complex form with differentiated segments, crypts and villi, as found in the adult. In this study, we characterized the expression of three peptide transporters (PepT1a, PepT1b, and PepT2) in the gastrointestinal tract of Mozambique tilapia ( Oreochromis mossambicus ) larvae along 12 days of development, from pre-hatching to the completion of yolk sac absorption. Gene expression analysis revealed differential and complimentary time-dependent expression of the PepT1 variants and PepT2 along the larval development period. Immunofluorescence analysis showed differential protein localization of the three peptide transporters (PepTs) along the gastrointestinal tract, in a similar pattern to the adult. In addition, PepT1a was localized in mucosal cells in the larvae esophagus, in much higher abundance than in the adults. The results of this study demonstrate specialization of intestinal sections and absorbance potential of the enterocytes prior to the onset of active exogenous feeding, thus pointing to an uncharacterized function and role of the gastrointestinal tract and its transporters during the larval period.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Peptide Transporters in the Primary Gastrointestinal Tract of Pre-Feeding Mozambique Tilapia Larva

et al. 2019

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“…In particular, in a large number of teleost fish species, zebrafish and other cyprinids included, the spatio-temporal expression of PepT1b intestinal mRNA largely varies during ontogeny, in response to nutritional states (e.g., food deprivation/re-feeding), dietary challenges, and/or environmental conditions (e.g., in freshwater/seawater adaptation), as well as under certain disease states (e.g., gut inflammation) [14–22, 26, 30, 51–63]. But, in the light of the most recent findings, the new view that PepT1a and PepT1b may both be expressed and operate in teleost fish models and similarly or differently respond to the various internal and external solicitations should always be taken into account (for details, see Table 4, and literature cited therein [43–48, 50]).…”

Section: Discussionmentioning

confidence: 99%

The peptide transporter 1a of the zebrafish Danio rerio, an emerging model in nutrigenomics and nutrition research: molecular characterization, functional properties, and expression analysis

et al. 2019

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BackgroundPeptide transporter 1 (PepT1, alias Slc15a1) mediates the uptake of dietary di/tripeptides in all vertebrates. However, in teleost fish, more than one PepT1-type transporter might function, due to specific whole genome duplication event(s) that occurred during their evolution leading to a more complex paralogue gene repertoire than in higher vertebrates (tetrapods).ResultsHere, we describe a novel di/tripeptide transporter in the zebrafish (Danio rerio), i.e., the zebrafish peptide transporter 1a (PepT1a; also known as Solute carrier family 15 member a1, Slc15a1a), which is a paralogue (78% similarity, 62% identity at the amino acid level) of the previously described zebrafish peptide transporter 1b (PepT1b, alias PepT1; also known as Solute carrier family 15 member 1b, Slc15a1b). Also, we report a basic analysis of the pept1a (slc15a1a) mRNA expression levels in zebrafish adult tissues/organs and embryonic/early larval developmental stages. As assessed by expression in Xenopus laevis oocytes and two-electrode voltage clamp measurements, zebrafish PepT1a, as PepT1b, is electrogenic, Na+-independent, and pH-dependent and functions as a low-affinity system, with K0.5 values for Gly-Gln at − 60 mV of 6.92 mmol/L at pH 7.6 and 0.24 mmol/L at pH 6.5 and at − 120 mV of 3.61 mmol/L at pH 7.6 and 0.45 mmol/L at pH 6.5. Zebrafish pept1a mRNA is highly expressed in the intestine and ovary of the adult fish, while its expression in early development undergoes a complex trend over time, with pept1a mRNA being detected 1 and 2 days post-fertilization (dpf), possibly due to its occurrence in the RNA maternal pool, decreasing at 3 dpf (~ 0.5-fold) and increasing above the 1–2 dpf levels at 4 to 7 dpf, with a peak (~ 7-fold) at 6 dpf.ConclusionsWe show that the zebrafish PepT1a-type transporter is functional and co-expressed with pept1b (slc15a1b) in the adult fish intestine. Its expression is also confirmed during the early phases of development when the yolk syncytial layer is present and yolk protein resorption processes are active. While completing the missing information on PepT1-type transporters function in the zebrafish, these results open to future investigations on the similar/differential role(s) of PepT1a/PepT1b in zebrafish and teleost fish physiology.

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“…Further, a growing number of genome scan studies concurrently point to HSP, and hereunder HSP70, as important candidate genes for local adaptation to salinity (e.g. Nielsen et al, 2009;Tine et al, 2010;Metzger et al, 2016;Kokou et al, 2019). HSP70 is coded by two different genes: a constitutive type (hsc70) that remains unchanged or slightly upregulated when cells are exposed to stress, and an inducible type, hsp70, which is upregulated upon stress.…”

Section: Introductionmentioning

confidence: 99%

Estimating salinity stress via hsp70 expression in the invasive round goby (Neogobius melanostomus): implications for further range expansion

2020

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Species invasions often occur on coasts and estuaries where abiotic conditions vary, e.g. salinity, temperature, runoff etc. Successful establishment and dispersal of non-indigenous species in many such systems are poorly understood, partially since the species tend to show genetic and ecological plasticity at population level towards many abiotic conditions, including salinity tolerance. Plasticity may be driven by shifting expression of heat shock proteins such as Hsp70, which is widely recognized as indicator of physical stress. In this study, we developed a qPCR assay for expression of the hsp70 gene in the invasive round goby (Neogobius melanostomus) and tested the expression response of fish collected from a brackish environment in the western Baltic Sea to three different salinities, 0, 10 and 30. hsp70 expression was highest in fresh water, indicating higher stress, and lower at brackish (ambient condition for the sampled population) and oceanic salinities, suggestive of low stress response to salinities above the population’s current distribution. The highest stress in fresh water was surprising since populations in fresh water exist, e.g. large European rivers and Laurentian Great Lakes. The results have implications to predictions for the species’ plasticity potential and possible range expansion of the species into other salinity regimes.

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Short- and long-term low-salinity acclimation effects on the branchial and intestinal gene expression in the European seabass (Dicentrarchus labrax)

Cited by 33 publications

References 41 publications

Peptide Transporters in the Primary Gastrointestinal Tract of Pre-Feeding Mozambique Tilapia Larva

Peptide Transporters in the Primary Gastrointestinal Tract of Pre-Feeding Mozambique Tilapia Larva

The peptide transporter 1a of the zebrafish Danio rerio, an emerging model in nutrigenomics and nutrition research: molecular characterization, functional properties, and expression analysis

Estimating salinity stress via hsp70 expression in the invasive round goby (Neogobius melanostomus): implications for further range expansion

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