Short- and long-term effects of heavy metals on phosphatase activity in soils: An ecological dose-response model approach

Doelman, P.; Haanstra, L.

doi:10.1007/bf00266485

Cited by 124 publications

(74 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Alkaline phosphatase activity is common in aquatic ecosystems and is especially sensitive to Cu(II) ions (Dohelman & Haanstra, 1989;Renella et al, 2003). Cu(II) also inhibits acid phosphatase activities and alters its kinetics in soil (Huang & Shindo, 2000;Renella et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Dominance of sphingomonads in a copper-exposed biofilm community for groundwater treatment

et al. 2007

View full text Add to dashboard Cite

The structure, biological activity and microbial biodiversity of a biofilm used for the removal of copper from groundwater were studied and compared with those of a biofilm grown under copper-free conditions. A laboratory-scale submerged fixed biofilter was fed with groundwater (2.3 l h "1 ) artificially polluted with Cu(II) (15 mg l "1 ) and amended with sucrose (150 mg l "1 ) as carbon source. Between 73 and 90 % of the Cu(II) was removed from water during long-term operation (over 200 days). The biofilm was a complex ecosystem, consisting of eukaryotic and prokaryotic micro-organisms. Scanning electron microscopy revealed marked structural changes in the biofilm induced by Cu(II), compared to the biofilm grown in absence of the heavy metal. Analysis of cell-bound extracellular polymeric substances (EPS) demonstrated a significant modification of the composition of cell envelopes in response to Cu(II). Transmission electron microscopy and energy-dispersive X-ray microanalysis (EDX) showed that copper bioaccumulated in the EPS matrix by becoming bound to phosphates and/or silicates, whereas copper accumulated only intracytoplasmically in cells of eukaryotic microbes. Cu(II) also decreased sucrose consumption, ATP content and alkaline phosphatase activity of the biofilm. A detailed study of the bacterial community composition was conducted by 16S rRNA-based temperature gradient gel electrophoresis (TGGE) profiling, which showed spatial and temporal stability of the species diversity of copper-exposed biofilms during biofilter operation. PCR reamplification and sequencing of 14 TGGE bands showed the prevalence of alphaproteobacteria, with most sequences (78 %) affiliated to the Sphingomonadaceae. The major cultivable colony type in plate counts of the copper-exposed biofilm was also identified as that of Sphingomonas sp. These data confirm a major role of these organisms in the composition of the Cu(II)-removing community. INTRODUCTIONCopper is an essential trace element for all species studied to date, including humans (Fraga, 2005). However, a large excess intake of Cu(II) in drinking water can cause nausea, gastric irritation and vomiting, while long-term copper intoxication generates liver disease and severe neurological defects in humans (Hotz et al., 2003;Uriu-Adams & Keen, 2005). Chronic ingestion of drinking water with high Cu(II) concentrations (over 3 mg l 21 ) is thus a potential health risk, especially for susceptible populations like children ( Abbreviations: DGGE, denaturing gradient gel electrophoresis; EDX, energy-dispersive X-ray microanalysis; EPS, extracellular polymeric substances; LOI, loss on ignition; SEM, scanning electron microscopy; TEM, transmission electron microscopy; TGGE, temperature gradient gel electrophoresis; UPGMA, unweighted pair grouping with arithmetic averages. input in freshwaters is generated from a number of anthropogenic activities (Cameron, 1992), where these ions sequentially accumulate in sediments, bacteria, tubicid worms and fish, followed by uptake by humans thro...

show abstract

Section: Discussionmentioning

confidence: 99%

Dominance of sphingomonads in a copper-exposed biofilm community for groundwater treatment

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Dose-response curves were fitted by log-logistic modeling (Doelman and Haanstra, 1989) using the Marquardt method (proc NLIN, SAS 9.1; NC, USA). Significant (Po0.05) inhibitory effects on amoA gene copy and transcript numbers were detected using Student's t-tests.…”

Section: Statistical Analysesmentioning

confidence: 99%

Bacteria, not archaea, restore nitrification in a zinc-contaminated soil

et al. 2009

View full text Add to dashboard Cite

Biological ammonia oxidation had long been thought to be mediated solely by discrete clades of b-and c-proteobacteria (ammonia-oxidizing bacteria; AOB). However, ammonia-oxidizing Crenarchaeota (ammonia-oxidizing archaea; AOA) have recently been identified and proposed to be the dominant agents of ammonia oxidation in soils. Nevertheless, the dynamics of AOB versus AOA, and their relative contribution to soil ammonia oxidation and ecosystem functioning on stress and environmental perturbation, remain unknown. Using a 3-year longitudinal field study and the amoA gene as a molecular marker, we demonstrate that AOB, but not AOA, mediate recovery of nitrification after zinc (Zn) contamination. Pristine soils showed approximately equal amoA gene copy numbers and transcript levels for AOB and AOA. At an intermediate Zn dose (33.7 mmol Zn per kg), ammonia oxidation was completely inhibited, and the numbers of AOB and AOA amoA gene copies and gene transcripts were reduced. After 2 years, ammonia oxidation in the field soils was fully restored to preexposure levels, and this restoration of function was concomitant with an increase of AOB amoA gene copy and gene transcript numbers. Analysis of the restored community revealed domination by a phylogenetically distinct Zn-tolerant Nitrosospira sp. community. In contrast, the numbers of AOA amoA gene copies and gene transcripts remained 3-and 10 4 -fold lower than recovered AOB values, respectively. Thus, although recent findings have emphasized a dominant role of archaea in soil-borne ammonia oxidation, we demonstrate that a phylogenetic shift within the AOB community drives recovery of nitrification from Zn contamination in this soil.

show abstract

“…Similar results were also reported by Doelman and Haanstra (1979) who found a significant reduction in dehydrogenase activity in a sandy soil treated with 375 µg Pb and did not observe any toxic effect in clay and peat soils. In their other studies (Doelman and Haanstra, 1989;Haanstra and Doelman, 1991) short-and long-term effects of metals on phosphatase and arysulsulphatase activities in 5 different soils were investigated. They reported that at the ED 50 value (ecological dose), toxicity did not decrease with time and in sandy soils, was approximately 2.6 mmol kgG 1 dry soil for Cd, Cu, and Zn.…”

Section: Soil Microbial Diversitymentioning

confidence: 99%

Effect of Metals Contamination on Soil Microbial Diversity, Enzymatic Activity, Organic Matter Decomposition and Nitrogen Mineralisation (A Review)

Khan¹

2000

Pakistan J. of Biological Sciences

View full text Add to dashboard Cite

Soil is home to remarkable diversity of life from microbes to moles and a well-established ecosystem among these different organisms exists. Microorganisms either independently or in association with other organisms are playing very important role in the plant nutrients recycling. Soil metal concentrations above certain limits disrupt this ecosystem and as a result plant nutrient cycling process. Metal contamination in soils cause proportions of some groups of microorganisms to increase than the others. Compared with bacteria certain groups of fungi are less affected by soil metal pollution. Phospholipid fatty acid (PLFA) measurements in metal contaminated soils show that within bacterial population, metals are more toxic to gram-positive than gram-negative bacteria. Significant reductions in enzymatic activities occur in soils contaminated with metals and generally metals are more toxic to intra-cellular enzyme activities (e.g. dehydrogenase) than extra-cellular activities (e.g. phosphatase). Similarly, enzymes produced by bacteria are more affected by metal contamination than those produced by fungi. Organic matter decomposition and nitrogen mineralisation, which are carried out by various groups of microorganisms can also be affected by increased metal concentrations in the soils.

show abstract

Short- and long-term effects of heavy metals on phosphatase activity in soils: An ecological dose-response model approach

Cited by 124 publications

References 22 publications

Dominance of sphingomonads in a copper-exposed biofilm community for groundwater treatment

Dominance of sphingomonads in a copper-exposed biofilm community for groundwater treatment

Bacteria, not archaea, restore nitrification in a zinc-contaminated soil

Effect of Metals Contamination on Soil Microbial Diversity, Enzymatic Activity, Organic Matter Decomposition and Nitrogen Mineralisation (A Review)

Contact Info

Product

Resources

About