Short- and long-term effects of refeeding on key enzyme activities in glycolysis–gluconeogenesis in the liver of gilthead seabream (Sparus aurata)

Metón, Isidoro; Fernández, Felipe; Baanante, Isabel V.

doi:10.1016/s0044-8486(03)00281-3

Cited by 150 publications

(92 citation statements)

References 15 publications

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“…These authors showed a significant decrease in its activity (27% and 37%) verified, respectively, in young mice (subjected to 1 month of caloric restriction --60% of ad libitum intake) and in old mice (subjected to 28 months of caloric restriction with the same daily caloric intake). The NZR PK activity results were also corroborated by a study in fish that were starved during 18 days, in which PK activity significantly decreased (Meton et al, 2003). PK revealed no influence of food restriction on its mRNA level in the NZ breed.…”

Section: Discussionsupporting

confidence: 58%

Feed restriction and genetic selection on the expression and activity of metabolism regulatory enzymes in rabbits

Harten

Cardoso

2010

Animal

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This work aims at the identification of relevant intermediate metabolism enzymes contributing to improved meat production due to genetic selection. A wild rabbit (WR) breed and a highly meat selected breed (New Zealand (NZ) rabbit) were used. Food restriction was used as an experimental condition so as to enhance differences within the metabolic pathways under study. During a period of 30 days, NZ and WR experimental breeds were subjected to, respectively, 40% and 60% ad libitum food restriction leading to 17.7% and 21.1% initial weight. Hepatic glycolytic, lipidic and protein regulatory enzyme activity, transcriptional and metabolite levels were determined. Insulin-like growth factor (IGF-1), triiodothyronine, and cortisol were also evaluated. In the glycolytic pathways, the NZ control rabbits presented a higher phosphofructokinase and pyruvate kinase activity level when compared to the WR, while the latter group showed a higher expression of glycogen synthase, although with less glycogen content. In the nitrogen metabolism, our results showed a lower activity level of glutamate dehydrogenase in WR when subjected to food restriction. Within the lipid metabolism, results showed that although WR had a significantly higher mRNA hepatic lipase, non-esterified fatty acid levels were similar between the experimental groups. NZ rabbits presented a better glycemia control and greater energy substrate availability leading to enhanced productivities in which triiodothyronine and IGF-1 played a relevant role.Keywords: enzyme activity, intermediate metabolism, rabbits, regulatory enzymes, feed restriction ImplicationsThe effects of feed restriction on the intermediate metabolism of breeds of rabbits selected for meat production induce an increase over non-selected rabbits on the efficiency of metabolic systems responsible for the homeostasis with reflection on the glycemia maintenance levels and on the excretion process of protein catabolism products. According to our results, seasonal draft periods inducing food restriction might not impair the use of highly productive breeds in upgrading animal production. Further studies are necessary regarding these metabolic pathways, including integrated genomic, transcriptomic and metabolomic approaches leading to results with practical consequences that are specifically required to upgrade livestock production in areas submitted to seasonal feedstuff shortage.

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Section: Discussionsupporting

confidence: 58%

Feed restriction and genetic selection on the expression and activity of metabolism regulatory enzymes in rabbits

Harten

Cardoso

2010

Animal

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“…This result was consistent with the previous researches in mammals [53]. In aquatic animals, some similar researches were also reported such as gilthead sea bream [54,55], carp [56]; grass carp [57] and channel catfish (Ictalurus punctatus) [58]. At the same time, other researchers have demonstrated that the above fact has not been observed in Senegalese sole (Solea senegalensis, Kaup) and eel (Anguilla anguilla) [59,60].…”

Section: Discussionsupporting

confidence: 90%

Molecular Characterization and Identification of Facilitative Glucose Transporter 2 (GLUT2) and Expression of Related Glycometabolism in Response to Different Starch Levels in Blunt Snout Bream (Megalobrama amblycephala)

Liang¹,

Wilson-Arop²,

Mi³

et al. 2017

Preprint

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Abstract:Facilitative glucose transporters (GLUT) are transmembrane transporter of proteins involved in glucose transport across the plasma membrane. To date, there is no information about glucose transporter 2 (GLUT2) in blunt snout bream (Megalobram aamblycephala). In this study, GLUT2 were cloned and characterized from blunt snout bream, and its-expression in response to diets with different carbohydrate levels (17.1%; 21.8%; 26.4%; 32.0%; 36.3% and 41.9% of dry matter). In this study, the full-length cDNA of GLUT2 was 2577 bp, including a 5'-untranslated region (UTR) of 73 bp, a 3'-UTR of 992 bp, and an open reading frame of 1512 bp, encoding a polypeptide of 503 amino acids with predicted molecular weight of 55.046 kDa and theoretical isoelectric point of 7.52. GLUT2 has twelve across the membrane area locating at 7-29; 71-93; 106-123; 133-155; 168-190; 195-217; 282-301; 316-338; 345-367; 377-399; 412-434; 438-460 aimo acids respectively.Conservative structure domains located at 12-477 aimo acids belonging to sugar porter family major facilitator superfamily (MFS) transporter. The blunt snout bream GLUT2 showed high identity to their orthologs in other fish species and mammals. Quantitative real-time (qRT)-PCR assays revealed that GLUT2 expression was high in the liver, intestine and kidney; highest in the liver. Compared with the control group (17.1%), high dietary carbohydrate levels (32.0%; 36.3% and 41.9%) resulted in high plasma glucose at 3h after Preprints (www.preprints.org) | NOT PEER-REVIEWED |

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“…The ubiquitous presence of 2 H in the body means that any tissue that can generate glucose via G6P and export it into blood can potentially contribute to the observed plasma glucose 2 H-enrichment pattern. For most fish species, including the seabass, hepatic glycogen stores are depleted during fasting (Metón et al, 2003;Pérez-Jiménez et al, 2007 being therefore unable to sustain significant rates of EGP via glycogenolysis as observed. During refeeding with fishmeal-based diet there is an increased dependence on gluconeogenesis for EGP and for the repletion of hepatic glycogen (Viegas et al, 2012), however the decreased contribution of G6P sources to blood glucose during refeeding compared to the fed state may suggest better utilization of dietary CHO.…”

Section: Sources Of Blood Glucose During Prolonged Fasting and Conseqmentioning

confidence: 95%

“…1). Transition of hepatic metabolism from fasting to feeding has provided insight into the functioning and responsiveness of fish species to dietary cues (Metón et al, 2003;Metón et al, 2004;Soengas et al, 2006;Pérez-Jiménez et al, 2007. Under a low CHO diet as the one used in the present study, GK activity was very low in fed seabass as reported previously (Enes et al, 2008b; Moreira et al, Table 2 Primer pairs used for the GK and G6Pase partial cDNA cloning by RT-PCR and primers used to assay GK and G6Pase mRNA levels by quantitative real-time RT-PCR analysis.…”

Section: Nutritional Regulation Of G6pase and Gkmentioning

confidence: 99%

Effects of food-deprivation and refeeding on the regulation and sources of blood glucose appearance in European seabass (Dicentrarchus labrax L.)

Viegas

Rito

González

et al. 2013

Comparative Biochemistry and Physiology Part A: Molecular &

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H 2 O Gluconeogenesis Glycogenolysis Glucose 6-phosphatase Glucokinase NMR Sources of blood glucose in European seabass (initial weight 218.0 ± 43.0 g; mean ± S.D., n = 18) were quantified by supplementing seawater with deuterated water (5%-2 H 2 O) for 72 h and analyzing blood glucose 2 H-enrichments by 2 H NMR. Three different nutritional states were studied: continuously fed, 21-day of fast and 21-day fast followed by 3 days of refeeding. Plasma glucose levels (mM) were 10.7 ± 6.3 (fed), 4.8 ± 1.2 (fasted), and 9.3 ± 1.4 (refed) (means ± S.D., n = 6), showing poor glycemic control. For all conditions, 2 H-enrichment of glucose position 5 was equivalent to that of position 2 indicating that blood glucose appearance from endogenous glucose 6-phosphate (G6P) was derived by gluconeogenesis. G6P-derived glucose accounted for 65 ± 7% and 44 ± 10% of blood glucose appearance in fed and refed fish, respectively, with the unlabeled fraction assumed to be derived from dietary carbohydrate (35 ± 7% and 56 ± 10%, respectively). For 21-day fasted fish, blood glucose appearance also had significant contributions from unlabeled glucose (52 ± 16%) despite the unavailability of dietary carbohydrates. To assess the role of hepatic enzymes in glycemic control, activity and mRNA levels of hepatic glucokinase (GK) and glucose 6-phosphatase (G6Pase) were assessed. Both G6Pase activity and expression declined with fasting indicating the absence of a classical counter-regulatory stimulation of hepatic glucose production in response to declining glucose levels. GK activities were basal during fed and fasted conditions, but were strongly stimulated by refeeding. Overall, hepatic G6Pase and GK showed limited capacity in regulating glucose levels between feeding and fasting states.

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Short- and long-term effects of refeeding on key enzyme activities in glycolysis–gluconeogenesis in the liver of gilthead seabream (Sparus aurata)

Cited by 150 publications

References 15 publications

Feed restriction and genetic selection on the expression and activity of metabolism regulatory enzymes in rabbits

Feed restriction and genetic selection on the expression and activity of metabolism regulatory enzymes in rabbits

Molecular Characterization and Identification of Facilitative Glucose Transporter 2 (GLUT2) and Expression of Related Glycometabolism in Response to Different Starch Levels in Blunt Snout Bream (Megalobrama amblycephala)

Effects of food-deprivation and refeeding on the regulation and sources of blood glucose appearance in European seabass (Dicentrarchus labrax L.)

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