Short- and long-term effects of nicotine and the histone deacetylase inhibitor phenylbutyrate on novel object recognition in zebrafish

Faillace, María Paula; Pisera‐Fuster, Antonella; Medrano, Matías Pedro; Bejarano, A C; Bernabeu, Ramón

doi:10.1007/s00213-017-4532-x

Cited by 24 publications

(25 citation statements)

References 49 publications

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“…Conversely, zebrafish preferentially spent more time near familiar than novel objects when complex objects of different sizes (LEGO® figures) were used 3 . These inconsistencies may be associated with differences in the experimental protocol used in zebrafish research, which are often complex and difficult to replicate since there are a large number of existing variables (e.g., different object shapes, sizes and textures and/or even the presence or absence of habituation) 22,24,26,27 . To circumvent these issues, we established a protocol aiming to reduce experimental variables allowing precise data reliability.…”

Section: Discussionmentioning

confidence: 99%

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Exploring Object Discrimination in Zebrafish: Behavioral Performance and Scopolamine-Induced Cognitive Deficits at Different Retention Intervals

et al. 2019

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The object discrimination test allows the testing of different memory retention periods. However, few behavioral endpoints have been measured in fish species such that retention is often assessed using a single parameter (time spent in object area). Here, we aimed to explore the object discrimination test in zebrafish by assessing their behavioral performance after 1 h or 24 h retention interval periods. To characterize putative interaction-like behaviors, fish were tested in the absence or presence of scopolamine (1 h before test session). Zebrafish were habituated for three consecutive days in the experimental tank and training session was performed for 10 min using two identical nonpreferred objects (black cube or sphere). After the retention intervals, a familiar object was replaced by a novel object (test session, 10 min). Fish were also exposed to the novel tank diving test to assess locomotion and anxiety-like behaviors. At 1 h retention interval, animals performed more circular-like investigation near the familiar object, whereas 24 h after training session, a prominent rapid investigation was observed when animals explore the non-familiar object. Because scopolamine abolished these phenotypes, as well as the increased time spent in the novel object area during the test without changing locomotion and anxiety-related parameters, the behavioral responses described here may predictively reflect interaction-like behaviors involved in object discrimination memory in zebrafish models.

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Section: Discussionmentioning

confidence: 99%

“…Moreover, the use of different protocols, object shapes, sizes, and colors among laboratories 22,24,26 associated with the lack of behavioral parameters to measure a proper interaction with objects, complicate the interpretation regarding preference or aversion to novel objects 27 .…”

Section: Introductionmentioning

confidence: 99%

Exploring Object Discrimination in Zebrafish: Behavioral Performance and Scopolamine-Induced Cognitive Deficits at Different Retention Intervals

et al. 2019

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“…Afterwards, an opaque partition between the fish and the stimuli was removed and the two side‐by‐side compartments became visually accessible: one containing the microcentrifuge tubes and the other kept empty (Figure G). The colours of the microcentrifuge tubes were chosen based on previous work that showed an innate zebrafish preference towards red and green objects . The percentage cumulative time fish spent near each compartment (objects vs empty) was used to calculate the object preference score [% T objects /(% T objects + % T empty )] and stimuli exploration score [( T objects + T empty )/ T total ].…”

Section: Methodsmentioning

confidence: 99%

“…Colours were switched between replicates to avoid a specific preference towards the colour. We used colour‐cue objects because it has been reported that zebrafish are better in discriminating colour changes than changes in object shape or size …”

Section: Methodsmentioning

confidence: 99%

Oxytocin receptor signalling modulates novelty recognition but not social preference in zebrafish

Ribeiro

Nunes

Gliksberg

et al. 2020

J Neuroendocrinology

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| INTRODUC TI ONNonapeptides are known to be important players in the regulation of social behaviour across taxa. 1 They evolved from a common ancestral molecule, the arginine vasotocin, after a gene duplication event in early-jawed fish. 2 One copy originated the vasopressin-like peptides, which retained the most primitive functions of the ancestral molecule, namely the regulation of osmotic balance. The other originated the oxytocin (OXT)-like peptides, which have greatly diverged across species, being recruited for diverse homeostatic processes, including parturition and lactation in mammals. Throughout evolution, these nonapeptides have also been involved in the regulation of social behaviours, with vasopressin being more involved in aggression and agonistic behaviours and OXT in affiliative behaviours and sociality. 3 AbstractSociality is a complex phenomenon that involves the individual´s motivation to approach their conspecifics, along with social cognitive functions that enable individuals to interact and survive. The nonapeptide oxytocin (OXT) is known to regulate sociality in many species. However, the role of OXT in specific aspects of sociality is still not well understood. In the present study, we investigated the contribution of the OXT receptor (OXTR) signalling in two different aspects of zebrafish social behaviour: social preference, by measuring their motivation to approach a shoal of conspecifics, and social recognition, by measuring their ability to discriminate between a novel and familiar fish, using a mutant zebrafish lacking a functional OXTR. Although oxtr mutant zebrafish displayed normal attraction to a shoal of conspecifics, they exhibited reduced social recognition. We further investigated whether this effect would be social-domain specific by replacing conspecific fish by objects. Although no differences were observed in object approach, oxtr mutant fish also exhibited impaired object recognition. Our findings suggest that OXTR signalling regulates a more general memory recognition of familiar vs novel entities, not only in social but also in a non-social domain, in zebrafish. K E Y W O R D Sautism spectrum disorders, oxytocin, social preference, social recognition, zebrafish S U PP O RTI N G I N FO R M ATI O NAdditional supporting information may be found online in the Supporting Information section.

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“…Zebra sh show multiple advantages, including high physiological and genetic homology to mammals, rapid development, ease of genetic and experimental manipulations, sensitivity to cognitive tests as well as cost-and space-effectiveness (18). Although zebra sh are newcomers to studies of learning and memory, they are capable of performing well in a range of learning tasks such as, avoidance learning (19), alternation spatial memory task (20), associative learning task (21), object recognition (22), and automated learning paradigm (23).…”

Section: Introductionmentioning

confidence: 99%

Mediterranean Natural Extracts Improved Cognitive Behavior in Zebrafish and Healthy Rats and Ameliorated LPS-induced Cognitive Impairment in a Sex Dependent Manner

Pusceddu¹,

Hernandez-Baixauli²,

Puiggrós³

et al. 2020

Preprint

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Background: Several findings suggest neuroinflammation as a contributing factor for the onset of psychiatric disorders such as Alzheimer’s disease, depression, and anxiety. There is increasing evidence pointing out that the Mediterranean diet influences brain and behavior. Mediterranean herbs and spices have been shown to be within those components of the Mediterranean diet involved in cognitive enhancement. Thus, we investigated the influence of Mediterranean natural extracts (MNE), Rosemary extract (RE) and Glycyrrhiza glabra root extract (GGRE), on cognitive behavior. Results: Adult zebrafish were exposed to RE or GGRE (100 and 250 mg/L) treatments. Both MNE improved memory retention during the T-maze test, although no improvements were observed during the novel object preference. Similarly, chronic administration of RE (150 mg/Kg) and GGRE (150 mg/Kg) improved, respectively, spatial and retention memory, as assessed by the Morris Water Maze (MWM), and the Elevated Plus Maze (EPM) in healthy male rats. However, no improvements were observed during the novel object recognition. Finally, male, and female rats were chronically treated with lipopolysaccharide [(LPS) 300 ug/kg] and orally administered with RE. Interestingly, RE reversed LPS-induced cognitive deficit during the MWM and EPM in female rats. Conclusions: We found that MNE improved cognition in both zebrafish and rats. Moreover, MNE rescued LPS-induced cognitive impairment in a gender-specific manner. Therefore, our study supports the view that zebrafish represent a valuable preclinical model for drug discovery in neuroscience. These findings contribute to an exciting and growing body of research suggesting that MNE may play an important role in the prevention of cognitive impairment.

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Short- and long-term effects of nicotine and the histone deacetylase inhibitor phenylbutyrate on novel object recognition in zebrafish

Cited by 24 publications

References 49 publications

Exploring Object Discrimination in Zebrafish: Behavioral Performance and Scopolamine-Induced Cognitive Deficits at Different Retention Intervals

Exploring Object Discrimination in Zebrafish: Behavioral Performance and Scopolamine-Induced Cognitive Deficits at Different Retention Intervals

Oxytocin receptor signalling modulates novelty recognition but not social preference in zebrafish

Mediterranean Natural Extracts Improved Cognitive Behavior in Zebrafish and Healthy Rats and Ameliorated LPS-induced Cognitive Impairment in a Sex Dependent Manner

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