The grapevine (Vitis spp.) is the one main fruit grown in the world. One of the primary requirements of production destined to be consumpted in natura is the acceptance of product in the consumer market. Thus, the aim of this work was to evaluate the physico-chemical characteristics and the sensorial acceptance of grapes 'Niágara Rosada' and 'Niágara Branca'. The experimental design was in a one-factor scheme, with two levels. The evaluated variables were bunch length, berry diameter, soluble solids content, pH and titratable acidity. For the sensorial evaluation, the attributes color, aroma, sweetness, acidity, flavor and overall acceptance were evaluated using a hedonic scale of nine points, besides the intention to buy. The 'Niágara Branca' presented higher mean for cluster length and 'Niágara Rosada' for berry diameter. For soluble solids and titratable acidity, 'Niágara Rosada' presented higher mean. In the sensory analysis, the 'Niágara Rosada' obtained the highest note for color and the 'Niágara Branca' for sweetness; however, the two cultivars had good overall acceptance, with grades between 8.3 and 8.4. It is concluded that the 'Niágara Rosada' and the 'Niágara Branca' presented good acceptance by the evaluators.