Shoot-regeneration method: a simplified method of evaluating sulfonylurea herbicide resistance

Section: Occurrence Of Sulfonylurea Resistance In Schoenoplectiella Jmentioning

confidence: 99%

Biology and mechanisms of sulfonylurea resistance in Schoenoplectiella juncoides, a noxious sedge in the rice paddy fields of Japan

Sada

Uchino

2017

“…In addition, a simpler method was proposed, based on the regrowth of roots or shoots from weed samples treated with SU herbicides (Boutsalis 2001; Hamamura et al . 2003; Ohno et al . 2004).…”

Section: Introductionmentioning

confidence: 99%

“…This method has been applied to several weed species (Simpson et al 1995;Lovell et al 1996;Uchino et al 1999;. In addition, a simpler method was proposed, based on the regrowth of roots or shoots from weed samples treated with SU herbicides (Boutsalis 2001;Hamamura et al 2003;Ohno et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Difference in ultraweak photon emissions between sulfonylurea‐resistant and sulfonylurea‐susceptible biotypes of Scirpus juncoides following the application of a sulfonylurea herbicide

Inagaki

Ishida

Uchino

et al. 2008

We examined the ultraweak photon emissions from a paddy weed, Scirpus juncoides, to assess the availability of photon emissions for the identification of weed biotypes resistant to sulfonylurea herbicides. The emission intensity from the plant organs increased when treated with a sulfonylurea herbicide in a concentration-dependent manner.The increment in emissions was higher in the sulfonylurea-resistant biotypes than in the sulfonylurea-susceptible biotypes.The difference between the biotypes was greater in the culms than in the roots and remained so through the vegetative growth stage to the flowering stage.This difference was independent of the seed source or mutations in the acetolactate synthase genes of the resistant biotypes.These results suggest that the determination of ultraweak photon emissions can be a useful method for identifying the sulfonylurea-resistant biotypes of S. juncoides.

“…), a shoot regeneration method (Ohno et al . ), an in vivo ALS assay (Uchino & Watanabe ) and DNA sequencing (Uchino et al . ) have been applied.…”

mentioning

confidence: 99%

“…For the diagnosis of SU resistance in S. juncoides, a rooting method (Hamamura et al 2003), a shoot regeneration method (Ohno et al 2004), an in vivo ALS assay and DNA sequencing ) have been applied. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is expected to be an effective diagnostic method as it is more rapid and less expensive in determining the mutated sites than DNA sequencing (Corbett & Tardif 2006).…”

mentioning

confidence: 99%

Rapid diagnosis of sulfonylurea‐resistant Schoenoplectus juncoides [Roxb.] Palla using polymerase chain reaction–restriction fragment length polymorphism and isogene‐specific direct sequencing

Sada

Ikeda

Kizawa

2012

Rapid diagnostic methods to detect known mutations in acetolactate synthase (ALS) genes that confer sulfonylurea (SU) resistance to Schoenoplectus juncoides were developed in this study. By using 11 SU‐resistant accessions (nine accessions with a Pro197 substitution in ALS1 or ALS2, one accession with an Asp376Glu substitution in ALS2 and one accession with a Trp574Leu substitution in ALS2), polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis for DNA fragments that were amplified simultaneously from genomic ALS1 and ALS2 and PCR–RFLP analysis for DNA fragments that were amplified from either of the genomic ALS1 or ALS2 were carried out. In each of the two PCR–RFLP analyses, a common PCR product was digested separately with the restriction enzymes, BspLI, MboI and MunI, in order to detect Pro197 substitutions, an Asp376Glu substitution and a Trp574Leu substitution, respectively. In each of the lanes where the detection of SU‐resistant substitutions was aimed, a specific band to suggest the existence of the said substitutions was observed in theoretically assumable ways. Separately, a direct sequencing method also was established, which was able to selectively sequence ALS1 or ALS2 from common templates containing both ALS1 and ALS2 by the isogene‐selective primers that were designed to anneal either of the ALS genes. It is expected that these methods could be used for the genetic analysis of SU‐resistant S. juncoides by providing rapid and accurate diagnosis.