Shoot regeneration and micrografting of micropropagated hybridtomatoes

Grigoriadis, Ioannis; Nianiou-Obeidat, Irini; Tsaftaris, Athanasios

doi:10.1080/14620316.2005.11511914

Cited by 8 publications

(5 citation statements)

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“…Felina required higher concentration of Z compared to Don Jose and Siena and showed greater regeneration frequency (94.4%), followed by Don Jose (92.5%) and Siena (83.33%) (Table 5, non-transformed plants). Similarly, high rates of regeneration were achieved using a combination of 0.5 mg/l Z and 0.1 mg/l IAA (Grigoriadis et al 2005), whereas MS with 1 mg/l Ζ and 0.5 mg/l IAA showed the highest percentage of regeneration (30.4%) in tomato hybrid Rio Grande (Yasmeen 2009). Herein, the three hybrids were similar in terms of regeneration efficiency, when grown in the best regeneration media (Table 2) confirming that the selection of the appropriate medium for the in vitro regeneration of tomato is genotype specific, which affects not only the efficiency of the regeneration, but also the genetic transformation (Ajenifujah-Solebo et al 2012).…”

Section: Resultsmentioning

confidence: 85%

Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids

Stavridou¹,

Τzioutziou²,

Madesis³

et al. 2019

CZECH J GENET PLANT

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The current study aimed to produce rootstock material through micropropagation by developing efficient regeneration and Agrobacterium-mediated transformation protocols for three high quality commercial tomato hybrids (Felina, Siena and Don Jose) to overexpress the GmGSTU4 gene from Glycine max L. previously shown to enhance antioxidant activity. We investigated the plant growth regulators zeatin (Z) and 3-idoleacetic acid (IAA) to determine their best combination for an efficient regeneration protocol for each hybrid. The highest regeneration efficiency was observed in Felina (94.4%) with 1.0 mg/l Z and 0.1 mg/l IAA. In contrast, Don Jose (92.5%) and Siena (83.3%) performed better with 0.5 mg/l Z and 0.1 mg/l IAA. The three hybrids did not differ in micropropagation index, however, Felina showed the highest number of in vitro rooted and in vivo acclimatized plants. Factors such as the age of explant, days in pre- and co-culture and the concentrations of acetosyringone and thiamine on Agrobacterium-mediated genetic transformation were assessed. The transformation indices were 37.04% for the Felina, 13.8% for Siena and 8.33% for Don Jose. We conclude that targeted genotype-specific regeneration protocols will provide an efficient and cost effective genetic transformation system for rootstock production and further incorporation into micropropagation and transgrafting systems.

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Section: Resultsmentioning

confidence: 85%

Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids

Stavridou¹,

Τzioutziou²,

Madesis³

et al. 2019

CZECH J GENET PLANT

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“…These expenses could be reduced through innovative production techniques. Micrografting has been recently introduced into the arena of tomato grafting and this technique uses micropropagated scion grafted onto 3-week-old rootstock seedlings (Grigoriadis et al, 2005). For propagators unable to carryout this type of advanced procedure, similar principles of reducing indirect costs could be used.…”

Section: Resultsmentioning

confidence: 99%

An Economic Analysis of Two Grafted Tomato Transplant Production Systems in the United States

Rivard

Sydorovych

O’Connell

et al. 2010

hortte

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The grafting of herbaceous vegetables is an emerging development in the United States. This report provides an estimate of the variable costs of grafting within U.S. tomato (Solanum lycopersicum) transplant production systems. Grafted and nongrafted plants were propagated at two commercial farming operations in Ivanhoe, NC (NC) and Strasburg, PA (PA) and the farm in NC produced certified organic transplants. Detailed economic production sequences were generated for each site, and grafted and nongrafted transplant production costs were $0.59 and $0.13 in NC, and $1.25 and $0.51 in PA, respectively. Direct costs associated with grafting (e.g., grafting labor, clips, chamber, etc.) accounted for 37% to 38% of the added cost of grafting, and grafting labor was 11.1% to 14.4% of the cost of grafted transplant production. Seed costs represented 52% and 33% of the added cost of grafting at the two sites, and indirect costs (e.g., soil, trays, and heating) accounted for 10% and 30% of the added cost of grafting. Our findings suggest that under current seed prices and with similar production practices, the feasibility of grafting in the United States is not disproportionately affected by domestic labor costs. Additionally, the economic models presented in this report identify the cost of production at various transplant stages, and provide a valuable tool for growers interested in grafted tomato transplant production and utilization.

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“…(See Voinnet 2005a for a recent review on non-cell autonomous RNA silencing). With the current increasing interest in using grafted plants in vegetable production (Sapountzakis and Tsaftaris 2002; c02_1 08/02/2007 Grigoriadis et al 2005), systemic RNA-silencing scion genes from rootstock RNAs (Lucas and Lee 2004;Athanasiadou et al 2005) is under intense study as a major determinant of lowering quality characteristics in vegetable fruits.…”

Section: Rna-based Control Mechanismsmentioning

confidence: 99%