Shoot organogenesis from leaf callus and ISSR assessment for their identification of clonal fidelity in Rhinacanthus nasutus (L.) Kurz., a potent anticancerous ethnomedicinal plant

Cheruvathur, Meena K.; Sivu, Ambikabai Raghavanpillai; Pradeep, N. S.; Thomas, T. Dennis

doi:10.1016/j.indcrop.2012.02.043

Cited by 19 publications

(11 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ISSR investigation of callus derived plants showed no variation compared to the mother plant and thus confirming that they are genetically uniform. Our results are in agreement with other reports where the absence of genetic variation of in vitro generated plants has been reported in other systems (Cheruvathur et al 2012). ISSR has high ability to expose polymorphism and present high potential to establish intra and inter genomic diversity as compared to other primers (Mishra et al 2011;Wang et al 2012).…”

Section: Discussionsupporting

confidence: 93%

Plant regeneration from organogenic callus and assessment of clonal fidelity in Elephantopus scaber Linn., an ethnomedicinal herb

Abraham

Thomas

2015

Physiol Mol Biol Plants

Self Cite

View full text Add to dashboard Cite

An efficient callus induction and plant regeneration system has been standardized for an ethnomedicinal plant, Elephantopus scaber Linn. Two explants i. e. seeds and leaf segments were used for callus induction. Murashige and Skoog (MS) medium supplemented with 5.0 μM 2, 4-dichlorophenoxy acetic acid (2, 4-D) and 0.5 μM kinetin (Kn) gave the optimum frequency (89 %) of callus induction from seed explant. The results showed that the highest response in terms of percent callus regenerating (91 %) and number of shoots (56) per culture was recorded on MS medium supplemented with 6.0 μM N6-benzylaminopurine (BA) and 1.5 μM α naphthalene acetic acid (NAA). The best rooting of regenerated shoots was obtained on half strength MS medium supplemented with 6.0 μM indole-3- butyric acid (IBA). On this medium, 100 % of the shoots produced roots with a mean number of 3.2 roots per shoot. The positive role of vesicular arbuscular mycorrhizae (VAM) along with potting mix has been well established in the present study. Of the various potting mix employed for plant acclimatization, the highest response of 100 % plant survival was noticed when autoclaved garden soil, sand (2:1) and VAM was utilized as potting mix. Inter-simple sequence repeats (ISSR) were used to establish the clonal fidelity of regenerated plantlets and the banding profiles from callus derived plants were monomorphic and similar to those of mother plant, thus ascertaining the true-to-type nature of these plants.

show abstract

Section: Discussionsupporting

confidence: 93%

Plant regeneration from organogenic callus and assessment of clonal fidelity in Elephantopus scaber Linn., an ethnomedicinal herb

Abraham

Thomas

2015

Physiol Mol Biol Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…This fact suggests that the alogamy of the matrices in the Caju reserve can cause a greater genetic distance between the progeny and the mother plant. The results of genetic divergence corroborate with the results obtained by Cheruvathur et al (2012), where the analysis of ISSR data in nine plants regenerated in vitro from R. nasutus callogenesis and the mother plant, the use of 12 ISSR markers indicated low genetic variability. In a study with ISSR markers in plants regenerated in vitro from the callogenesis, Perera et al (2015) concluded that these are useful in detecting polymorphisms in Miscanthus × Giganteus and can be used for generation and screening of mutants leading to the development of new cultivars.…”

Section: Molecular Analysissupporting

confidence: 86%

“…The results of genetic divergence corroborate the results obtained by Cheruvathur et al (2012), where the analysis of ISSR data in nine plants regenerated in vitro from callogenesis of R. nasutus and mother plant, the use of 12 ISSR markers indicated low genetic variability. The combination of phytochemical studies and genetic similarity favors the identification of more divergent individuals with characteristics of interest.…”

Section: Molecular Analysissupporting

confidence: 86%

Antioxidant Activity, Rutin Content and Genetic Similarity Between Matrices and Progenies of Hancornia speciosa

Machado¹,

Oliveira²,

Oliveira³

et al. 2018

JAS

View full text Add to dashboard Cite

Mangaba tree (Hancornia speciosa Gomes) is a tropical fruit species from Brazil and presents socioeconomic potential. The objective of this study was to determine antioxidant activity, rutin content and genetic similarity among in vivo and in vitro matrices and progenies of six accessions from the Mangaba Active Germplasm Bank of the Embrapa Coastal Tablelands, SE, Brazil. Young leaves of adult matrix plants and in vitro callus methanolic extracts obtained from young leaves resulted in differences between the accessions for the rutin content and high antioxidant activity. The Costa Azul accession, from Bahia, Brazil outstood with values above 3,000 g of dry extract/g of DPPH (2.2-diphenyl-1-picrilhydrazyl) in in vitro callus coming from nodal and internodal segments (3,023.73 and 3,136.80 g of dry extract/g of DPPH, respectively). Rutin was not found in extracts resulting from in vitro callus of mangaba tree. The difference among the values obtained for DPPH analysis are superior when compared with in vivo leaf extract, with the in vitro Costa Azul accession (nodal and internodal) outstanding, concluding that in vitro callus induction can interfere with chemical compounds of the plant. The markers detect genetic similarity under in vitro cultivation conditions. The Costa Azul accession group itself in isolation from others and EC 50 concentrations differentiated between leaf and callus are obtained. O EC 50 is superior in extracts deriving from in vitro callus, with the in vitro Costa Azul accession (nodal and internodal).

show abstract

“…However, the highest callus regeneration (96%) was observed in MS medium, supplemented with 3 mgL -1 Kn and 0.5 mgL -1 NAA. An important finding in this study was that the ISSR (Inter Simple Sequence Repeat) analysis of R. nasutus results in shoots with low degree of variation and statistically not significant differences [50].…”

Section: Micropropagation Of Medicinal Speciesmentioning

confidence: 59%

Polyamines, Gelling Agents in Tissue Culture, Micropropagation of Medicinal Plants and Bioreactors

Lima¹,

Campos²,

Willadino³

et al. 2012

Recent Advances in Plant in Vitro Culture

View full text Add to dashboard Cite

Currently, tissue cultures of species of agricultural importance have wide applicability in industrial production processes. Tissue culture is a name given to a set of techniques that allow the regeneration of cells, tissues and organs of plants, from segments of plant organs or tissues, using nutrient solutions in aseptic and controlled environment. This regeneration is based on the totipotency of plant cells. Totipotency is a capability indicating that plant cells, in different times, may express the potential to form a new multicellular individual. Tissue culture appears to be a good alternative to conventional propagation, requiring less physical space, with high multiplication rate, without incidence of pests and diseases during cultivation, and enabling higher control of the variables involved. Thus, in the in vitro environment, with the required stimuli and appropriate conditions, different cell types express different behaviors, possibly leading to cell multiplication and differentiation into a specific tissue, characterized by a form and a function, which may lead to the regeneration of a new individual

show abstract

Shoot organogenesis from leaf callus and ISSR assessment for their identification of clonal fidelity in Rhinacanthus nasutus (L.) Kurz., a potent anticancerous ethnomedicinal plant

Cited by 19 publications

References 36 publications

Plant regeneration from organogenic callus and assessment of clonal fidelity in Elephantopus scaber Linn., an ethnomedicinal herb

Plant regeneration from organogenic callus and assessment of clonal fidelity in Elephantopus scaber Linn., an ethnomedicinal herb

Antioxidant Activity, Rutin Content and Genetic Similarity Between Matrices and Progenies of Hancornia speciosa

Polyamines, Gelling Agents in Tissue Culture, Micropropagation of Medicinal Plants and Bioreactors

Contact Info

Product

Resources

About