Shoot initiation and multiplication from a mature tree of Terminalia arjuna roxb

Pandey, Sandeep; Singh, Mahesh Kumar; Jaiswal, Uma; Jaiswal, Vivek

doi:10.1079/ivp2006790

Cited by 34 publications

(29 citation statements)

References 14 publications

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“…Hence, ½ MS medium was used for establishing primary cultures from nodal segments. Similar to the present observation, low salt concentration was reported to be superior for shoot proliferation in Terminalia arjuna (Pandey et al 2006) and Azadirachta indica (Chaturvedi et al 2004).…”

Section: Resultssupporting

confidence: 91%

“…Explants harvested during other periods were found to be less responsive and with high infection percentage. The effect of season on bud sprouting has also been reported previously in Crataeva adansonii (Sharma et al 2003), Terminalia arjuna (Pandey et al 2006), Salvadora persica (Phulwaria et al 2011) and Dalbergia sissoo (Vibha et al 2013).…”

Section: Resultssupporting

confidence: 79%

“…4f). Optimum rooting response using IBA has been reported for many trees including T. arjuna (Pandey et al 2006), Azadirachta indica (Chaturvedi et al 2004) and T. chebula well-developed shoot and roots were taken out and washed gently to remove traces of agar and transferred to earthen pots containing garden soil and organic manure in 1:1 ratio. These pots were initially covered by polythene bags to maintain humidity and kept at 26±2°C.…”

Section: Resultsmentioning

confidence: 99%

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Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

Dangi

Khurana-Kaul

Kothari

et al. 2014

Physiol Mol Biol Plants

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The present study reports an efficient in vitro micropropagation protocol for a medicinally important tree, Terminalia bellerica Roxb. from nodal segments of a 30 years old tree. Nodal segments taken from the mature tree in MarchApril and cultured on half strength MS medium gave the best shoot bud proliferation response. )] in the culture medium aided to minimize browning and improve explant survival during shoot bud induction. Highest multiplication of shoots was achieved on medium supplemented with 6-benzyladenine (BA, 8.8 μM) and α-naphthalene acetic acid (NAA, 2.6 μM) in addition to antioxidants. Shoot elongation was obtained on MS medium containing BA (4.4 μM)+phloroglucinol (PG, 3.9 μM). Elongated shoots were transferred to half strength MS medium containing indole-3-butyric acid (IBA, 2.5 μM) for root development. The acclimatization of plantlets was carried out under greenhouse conditions. The genetic fidelity of the regenerated plants was checked using inter simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) analysis. Comparison of the bands among the regenerants and mother plant confirmed true-to-type clonal plants.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 79%

Section: Resultsmentioning

confidence: 99%

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Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

Dangi

Khurana-Kaul

Kothari

et al. 2014

Physiol Mol Biol Plants

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“…Para el género Terminalia, Ramesh et al, (2005) lograron el establecimiento exitoso de segmentos nodales de Terminalia bellirica; Pandey et al, (2006) lograron el establecimiento de nudos de Terminalia arjuna y Méndez y Abdelnour (2014) lograron el establecimiento in vitro de segmentos nodales. Para botarrama blanco no existen trabajos de establecimiento in vitro de segmentos nodales.…”

Section: Introductionunclassified

Establecimiento in vitro de dos especies nativas de Costa Rica: Terminalia amazonia (Amarillón) y Vochysia allenii (Botarrama Blanco)

Gómez¹,

Rojas-Vargas²,

Gradaille³

2014

Rev. Colomb. Biotecnol.

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ResumenEl objetivo de esta investigación fue lograr el establecimiento in vitro de la especie Terminalia amazonia y Vochysia allenii debido a la dificultad de propagarlas sexual y asexualmente con técnicas convencionales. Se logró establecer segmentos nodales de ambas especies en condiciones in vitro empleando el HgCl2 0,1 % con un tiempo de exposición de 5 minutos. El mejor medio de cultivo para nudos fue el WPM 100 % de sales para T.amazonia y para V. allenii fue el WPM 50 % de sales. Después de 28 días de cultivo se obtuvo un 42 % de nudos establecidos para T. amazonia y 10% para V. allenii. En ambas especies se evaluó el efecto sobre la brotación de cinco concentraciones de 6-bencilaminopurina (6-BAP) (0,0; 2. AbstractThe objective of this research was to achieve the in vitro establishment of the species Terminalia amazonia and Vochysia allenii due to the difficulty propagate sexually and asexually with conventional techniques. It was possible to establish the nodal segments of both species in in vitro conditions using 0.1 % HgCl2 with an exposure time of 5 minutes. The best nodal culture medium was 100 % WPM salts in T. amazonia and V. allenii was WPM 50% salts. After 28 days of culture 42 % of nodal segments to T. amazonia and V. allenii 10% was obtained. In both species, the effect on sprouting of five concentrations of 6-benzylaminopurine (6-BAP) (0.0, 2.22, 4.44, 6.66, 8.88 μM L -1 ) and five thidiazuron (TDZ) (0.0, 0.22, 0.45, 0.68, 0.90 μM L -1 ) was evaluated. Outbreak was scored on average per explant in the five treatments of BAP and TDZ used.

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“…The application of micropropagation is well documented in the literature with respect to production of ornamentals (Lu et al, 1990;Ahmed et al, 2002), food crops (Compton and Gray, 1993;Gopal et al, 1998;Shirin et al, 2007) and forest trees (Gupta et al, 1980, Pandey et al, 2006, Agrawal and Sandar, 2007.…”

Section: Introductionmentioning

confidence: 99%

Studies on Hollarrhena floribunda (G. Don) Durand Schinz: Germination and seedling growth, and preliminary investigations on callus induction and plant regeneration

Ayisire¹,

Ayisire²,

Akinbola³

2012

Afr. J. Agric. Res.

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Shoot initiation and multiplication from a mature tree of Terminalia arjuna roxb

Cited by 34 publications

References 14 publications

Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers

Establecimiento in vitro de dos especies nativas de Costa Rica: Terminalia amazonia (Amarillón) y Vochysia allenii (Botarrama Blanco)

Studies on Hollarrhena floribunda (G. Don) Durand Schinz: Germination and seedling growth, and preliminary investigations on callus induction and plant regeneration

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