Shoot histogenesis in tobacco callus cultures

Maeda, Eizo; Thorpe, Trevor A.

doi:10.1007/bf02618409

Cited by 43 publications

(20 citation statements)

References 14 publications

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“…The developmental pathway leading to the initiation and growth of shoots from tobacco callus has been reported previously [11,24]. These studies have revealed that although not completely synchronous, the major histological and physiological events accompanying the process follow a precise temporal pattern and occur during the first two weeks in culture.…”

Section: Introductionmentioning

confidence: 96%

“…Tracheary elements appear in the areas between the cell files and inoculum around day 9 and this is followed by the formation of meristemoids which will develop further into shoot primordia around day 12. Shoots appear as protuberances after day 14 and formation of leaves can be observed around day 21 [11]. This developmental sequence has been associated with physiological events which have revealed an increased respiratory rate occurring in tissue destined to form shoots.…”

Section: Introductionmentioning

confidence: 96%

“…These studies have revealed that although not completely synchronous, the major histological and physiological events accompanying the process follow a precise temporal pattern and occur during the first two weeks in culture. The organogenic process is initiated by heavy accumulation of starch in the parenchyma cells destined to form shoots [23], followed by the formation of cell files around the inoculum [11]. Tracheary elements appear in the areas between the cell files and inoculum around day 9 and this is followed by the formation of meristemoids which will develop further into shoot primordia around day 12.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Changes in the de novo, salvage, and degradation pathways of pyrimidine nucleotides during tobacco shoot organogenesis

Loukanina

Stasolla

Belmonte

et al. 2008

Plant Physiology and Biochemistry

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Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Changes in the de novo, salvage, and degradation pathways of pyrimidine nucleotides during tobacco shoot organogenesis

Loukanina

Stasolla

Belmonte

et al. 2008

Plant Physiology and Biochemistry

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“…Shoot regeneration from tobacco calli has been studied by Thorpe and his co-workers Murashige, 1968, 1970;Meier, 1972, 1973;Maeda and Thorpe, 1979). In most of these studies, subcultured calli regenerated a number of shoots after transfer to shoot formation medium.…”

Section: Discussionmentioning

confidence: 99%

“…Although in tobacco (Nicotiana tabacum L.) culture, the developmental sequence leading to the formation of shoots from calli has already been reported (Thorpe and Murashige, 1970;Maeda and Thorpe, 1979), the size of the calli used in these experiments was too large to analyze the shoot formation mechanism precisely. Although regeneration of shoots from small calli derived from mesophyll protoplasts has been reported (Hayashi and Nakajima,1984;Firoozabady,1986), the protoplast isolation and culture require very complex procedures and techniques.…”

Section: Introductionmentioning

confidence: 99%

Simple and Rapid Regeneration of Shoot and Root from Micro-Callus Cultures in Nicotiana tabacum L.

Tsuyuki

Kikuchi

1999

Plant Biotechnology

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An in-vitro procedure is described for simple and rapid regeneration of shoots and roots from small calli of tobacco (Nicotiana tabacum L. cv. Xanthi). The calli induced from stem pith tissue were subcultured for 2 to 3 times at 2-week intervals. Then, the calli were crushed on a stainless-steel mesh with a stainless-steel spoon and passed through the mesh. Micro-calli of uniform size (0.5 or 1.0 mm in diameter) were obtained by this procedure. For shoot regeneration, micro-calli of 0.5 mm in diameter were suspended in a liquid MS medium supplemented with 0.5 mg l-1 IBA and 0.05 mg l-1 BA and rotation-cultured. After 7 days, the culture bottles were transferred to static culture under continuous light of 5400 lux. After 11 days of static culture, an adventitious shoot was formed in about 50% of the calli. The diameter of shoot-forming calli was 1 to 2 mm and the number of shoots per callus was usually one. For root regeneration, the micro-calli (1.0 mm in diameter) were rotation-cultured in the dark for 5 days in a liquid MS medium supplemented with 1.0 mg l-1 NAA and 0.1 mg l-1 kinetin, and then put separately on a 0.06-ml Gelrite drop containing the same medium composition and cultured in darkness. A regeneration frequency of about 45% was obtained by day 30 of microculture. The diameter of calli at root initiation was 4 to 5 mm and the number of roots per callus was usually one or two.

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Origin of Adventitious Shoots Regenerated From Cultured Tobacco Leaf Tissue

Marcotrigiano

1986

American J of Botany

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Leaf discs from Nicotiana tabacum L., N. glauca Grahm., and a series of interspecific periclinal chimeras were cultured on Murashige and Skoog (MS) medium containing either 2.5 mg/1 6‐benzylaminopurine(BA) or 3.0 mg/16‐furfurylaminopurine (kinetin). Most shoots regenerated from chimeral leaf discs were non‐chimeral but 51 of 658 shoots were chimeral. The histogenic composition of plants regenerated from leaf discs of periclinal chimeras indicated that any cell layer in a leaf can be the ultimate source of shoots, and that shoots can be multicellular in origin. Certain periclinal arrangements were recovered more frequently and their chimeral nature was more stable during subsequent shoot growth. While N. tabacum leaf discs regenerated shoots on MS medium supplemented with either BA or with kinetin, N. glauca leaf discs did not form shoots on the medium containing kinetin. However, chimeras which possessed cells of both species arose on medium containing BA or kinetin, indicating that the morphogenetically competent (i.e., able to produce shoots in culture), N. tabacum cells either interacted with N. glauca cells leading to their competence or incorporated non‐competent N. glauca cells into nascent shoot apical meristems.

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Shoot histogenesis in tobacco callus cultures

Cited by 43 publications

References 14 publications

Changes in the de novo, salvage, and degradation pathways of pyrimidine nucleotides during tobacco shoot organogenesis

Changes in the de novo, salvage, and degradation pathways of pyrimidine nucleotides during tobacco shoot organogenesis

Simple and Rapid Regeneration of Shoot and Root from Micro-Callus Cultures in Nicotiana tabacum L.

Origin of Adventitious Shoots Regenerated From Cultured Tobacco Leaf Tissue

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