Shoot Development through Modified Transverse Thin Cell Layer (tTCL) Culture of Phalaenopsis Hybrid Protocorms

Lo, Kuo-Chin; Gansau, Jualang Azlan; Shih, C. S.; Kao, C. Y.

doi:10.3390/horticulturae8030206

Cited by 10 publications

(4 citation statements)

References 31 publications

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“…The similar finding of inhibitory effect of BAP on the conversion of protocorms into plantlets and root formation was reported in Dendrobium nobile (Nayak et al 2002), Dendrobium huoshanense (Luo et al 2009) and Doritis pulcherrima (Mondal et al 2013). In contrast to these results BAP also stimulated the formation of highest number of shoots in Dendrobium chryseum (Maharjan et al 2020), highest percentage of callus in Dendrobium barbatulum (Ashok Pyati, 2020) and highest mean values of PLBs in Phalaenopsis hybrid (Lo et al 2022). However, NAA was showed inhibitory effect to induce either EC or SEs in the medium.…”

Section: Pyatisupporting

confidence: 80%

In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

Pyati¹

2022

Plant Tissue Cult. & Biotech.

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An efficient in vitro regeneration protocol through somatic embryogenesis was established from longitudinally bisected protocorm (lTCL) of an endangered orchid Dendrobium ovatum. The efficiency of EC and SEs from the protocorms significantly relied on the concentration of PGRs. MS medium supplemented with TDZ (1.0 mg/l) induced optimum of EC (31.8%) and SEs (28.1/explant). Similarly, ZEA (0.5 mg/l) induced EC (27.6%) and SEs (17.1/explant). The combined treatment of TDZ (1.0 mg/l) and NAA (0.5 mg/l) resulted in the maximum induction of EC (58.6%) and SEs (39.8/explant) in an upright incubated explant. In another combined effect of ZEA (1.0 mg/l) and NAA (0.5 mg/l) induced EC (43.8%) and SEs (24.3/explant), whereas the combination of BAP (0.5 mg/l) and NAA (0.5 mg/l) produced EC (34.4%) and SEs (16.8/explants). The induced EC and SEs where healthier on medium containing TDZ + NAA than on the medium containing ZEA+NAA and BAP+NAA. The orientation of lTCL explants seemed to interact with position to affect the morphogenesis. The lTCL explants incubated in the upright orientation induced higher percentage of EC and a greater number of SEs/explants (EC-58.6% and SEs-39.8/explants) than that in the inverted orientation (EC- 16.4% and SEs-9.1/explants) irrespective of the PGRs. The SEs developed from the EC and the intermediate stage of PLBs were finally differentiated into plantlets. About 89% of plantlets were successfully acclimatized in the green house conditions. Plant Tissue Cult. & Biotech. 32(1): 53-66, 2022 (June)

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Section: Pyatisupporting

confidence: 80%

In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

Pyati¹

2022

Plant Tissue Cult. & Biotech.

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“…There are reports in which material was harvested from aseptic mother stock maintained on basal medium and TCL sections were aseptically excised (Bacopa monnieri [10]; Hadrolaelia grandis [11]; Paphiopedilum callosum [12]; Dendrobium spp. [13]; Begonia tuberous [14]; orchid Phalaenopsis hybrid protocorms [15]; Malus spp. sprouts [16,17]).…”

Section: Explant Selection and Preparation Of Thin Sectionsmentioning

confidence: 99%

Thin Cell Layer Tissue Culture Technology with Emphasis on Tree Species

Sharma,

Magotra,

Chourasia

et al. 2023

Forests

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An increased dependency on plant-based resources for food, shelter, and medicinal usage has increased their sustainable and unsustainable exploitation. To use this resource sustainably, plant tissue culture (PTC) is one important technology. Among different PTC techniques, thin cell layer (TCL) technology is a relatively simple and easily adaptable technique for in vitro cultures of plants. This technique uses small explants about 0.5–2 mm in thickness excised from different plant organs. It has been successfully used in the large-scale propagation of vegetables, legumes, and plants with medicinal benefits. TCL technology has proven to be effective in stimulating various organogenic responses when combined with various new methods such as nanotechnology or microtome-based explantation, especially in tree species. It is considered an important tool in plant biotechnology. Although the morphogenetic response per explant is usually higher in conventional explants, the appropriate use of plant growth regulators and geometric factors in TCL has the potential to make it more efficient and beneficial. This article provides an overview of the concept of TCL as applied to different plant species, particularly trees, since there are few, if any, summaries of TCL technology, especially in trees. This review will certainly revitalize this important technology so that it can be used effectively for successful mass propagation in the field of plant tissue culture.

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“…In many studies, protocorms were used for propagation and production of seedlings for several genera of orchids such as Phalaenopsis and Cymbidium (Lo et al, 2022;Regmi et al, 2017). This can be explained by the fact that protocorm cells have the ability to divide, which makes them ideal explants in the process of clonal micropropagation and studying the morphology of growth and development of representatives of the orchid family (Chookoh et al, 2019;Yeung, 2017).…”

Section: Figurementioning

confidence: 99%

Micropropagation of Trichopilia suavis Lindl. & Paxton

Hussien,

Molkanova,

Mitrofanova

2023

Ornam. Hortic.

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Trichopilia suavis is one of the important representatives of the genus Trichopilia, which can be used as a potted plant for the interior design. also, its beautiful inflorescences are added unusual element to bouquet compositions and actively used in the production of perfumes. This study was carried out in order to develop and improve micropropagation method for mass clonal production of T. suavis through protocorm culture. Half-strength Murashige and Skoog culture medium (½) has been modified by adding different organic components and growth regulators. The protocorms obtained from seeds germinated by asymbiotic method were used as explants. The results showed that the optimal treatment for formation of new protocorms (7.50 ± 0.7 units per protocorm) was obtained by culture on ½ MS medium with 0.5 mg L-1 of 6-Benzylaminopurine (6-BAP) and 100 mL L-1 of coconut water. At the stage of rooting, the highest number and length of roots (7.0 ± 0.45 units per plantlet, 2.64 ± 0.18 cm) were obtained through subsequent cultivation of plantlets on ½ MS medium with 0.5 mg L-1 indolyl-3-butyric acid (IBA), 1.0 g L-1 charcoal with the addition of 50 g L-1 of banana puree. The obtained seedlings were successfully adapted in a substrate consisting of bark, perlite and peat in a ratio of 1:1:1.

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Shoot Development through Modified Transverse Thin Cell Layer (tTCL) Culture of Phalaenopsis Hybrid Protocorms

Cited by 10 publications

References 31 publications

In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

Thin Cell Layer Tissue Culture Technology with Emphasis on Tree Species

Micropropagation of Trichopilia suavis Lindl. & Paxton

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