Shockwave Therapy Efficiently Cures Multispecies Chronic Periodontitis in a Humanized Rat Model

Datey, Akshay; Thaha, C. S. Adeeb; Patil, Suvarna; Gopalan, Jagadeesh; Chakravortty, Dipshikha

doi:10.3389/fbioe.2019.00382

Cited by 9 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 32 A possible explanation for the non-bactericidal effect of the low dose ESWT itself in the Wanner study 32 could be the lower energy, although to date there have been no comparative studies between low dose or focused high-energy shockwave therapy. Equivalent to the findings of our study, Gnanadhas et al 24 and Datey et al 25 could show an enhanced susceptibility of bacterial biofilms toward antibiotic treatment after shockwave treatment in cases of urinary catheter infection and periodontitis. 24 , 25 …”

Section: Discussioncontrasting

confidence: 62%

“…Equivalent to the findings of our study, Gnanadhas et al 24 and Datey et al 25 could show an enhanced susceptibility of bacterial biofilms toward antibiotic treatment after shockwave treatment in cases of urinary catheter infection and periodontitis. 24 , 25 …”

Section: Discussioncontrasting

confidence: 62%

“…17 However, in line with our results, there is evidence for fhESWT to have a direct disruptive and bactericidal effect on bacterial biofilms, although these data have not been of direct relevance to orthopaedic implants, but rather to urinary catheters and periodontitis. 24 , 25 …”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Antibiofilm efficacy of focused high-energy extracorporeal shockwaves and antibiotics in vitro

Milstrey

Roßlenbroich

Everding

et al. 2021

Bone & Joint Research

View full text Add to dashboard Cite

Aims Biofilm formation is one of the primary reasons for the difficulty in treating implant-related infections (IRIs). Focused high-energy extracorporeal shockwave therapy (fhESWT), which is a treatment modality for fracture nonunions, has been shown to have a direct antibacterial effect on planktonic bacteria. The goal of the present study was to investigate the effect of fhESWT on Staphylococcus aureus biofilms in vitro in the presence and absence of antibiotic agents. Methods S. aureus biofilms were grown on titanium discs (13 mm × 4 mm) in a bioreactor for 48 hours. Shockwaves were applied with either 250, 500, or 1,000 impulses onto the discs surrounded by either phosphate-buffered saline or antibiotic (rifampin alone or in combination with nafcillin). The number of viable bacteria was determined by quantitative culture after sonication. Representative samples were taken for scanning electron microscopy. Results The application of fhESWT led to a ten-fold reduction in bacterial counts on the metal discs for all impulse numbers compared to the control (p < 0.001). Increasing the number of impulses did not further reduce bacterial counts in the absence of antibiotics (all p > 0.289). Antibiotics alone reduced the number of bacteria on the discs; however, the combined application of the fhESWT and antibiotic administration further reduced the bacterial count compared to the antibiotic treatment only (p = 0.032). Conclusion The use of fhESWT significantly reduced the colony-forming unit (CFU) count of a S. aureus biofilm in our model independently, and in combination with antibiotics. Therefore, the supplementary application of fhESWT could be a helpful tool in the treatment of IFIs in certain cases, including infected nonunions. Cite this article: Bone Joint Res 2021;10(1):77–84.

show abstract

Section: Discussioncontrasting

confidence: 62%

Section: Discussioncontrasting

confidence: 62%

See 1 more Smart Citation

Antibiofilm efficacy of focused high-energy extracorporeal shockwaves and antibiotics in vitro

Milstrey

Roßlenbroich

Everding

et al. 2021

Bone & Joint Research

View full text Add to dashboard Cite

show abstract

“…This model has also been replicated by other research groups with different applications, such as the study of the effect of shock waves on multi-species oral biofilms [ 64 ], or the in vitro effect of replacing amoxicillin with penicillin V, when used in combination with metronidazole, in a biofilm model [ 65 ].…”

Section: Oral Biofilms and In Vitro Biofilm Modelsmentioning

confidence: 97%

Relevance of Biofilm Models in Periodontal Research: From Static to Dynamic Systems

et al. 2021

View full text Add to dashboard Cite

Microbial biofilm modeling has improved in sophistication and scope, although only a limited number of standardized protocols are available. This review presents an example of a biofilm model, along with its evolution and application in studying periodontal and peri-implant diseases. In 2011, the ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) research group at the University Complutense of Madrid developed an in vitro biofilm static model using representative bacteria from the subgingival microbiota, demonstrating a pattern of bacterial colonization and maturation similar to in vivo subgingival biofilms. When the model and its methodology were standardized, the ETEP research group employed the validated in vitro biofilm model for testing in different applications. The evolution of this model is described in this manuscript, from the mere observation of biofilm growth and maturation on static models on hydroxyapatite or titanium discs, to the evaluation of the impact of dental implant surface composition and micro-structure using the dynamic biofilm model. This evolution was based on reproducing the ideal microenvironmental conditions for bacterial growth within a bioreactor and reaching the target surfaces using the fluid dynamics mimicking the salivary flow. The development of this relevant biofilm model has become a powerful tool to study the essential processes that regulate the formation and maturation of these important microbial communities, as well as their behavior when exposed to different antimicrobial compounds.

show abstract

“…Thus, there is a strong need to determine the most optimal preventive therapies for periodontitis and perform research on regenerative treatments using appropriate animal models (Liu et al, 2018;Kourtzelis et al, 2019). For example, Datey et al used a rat periodontitis model and tested the effect of shockwave treatment in combination with antimicrobials on periodontitis (Datey et al, 2019). Apart from the significance of studying periodontitis itself, it is also a suitable model for studying bone regeneration and investigating the potential therapeutic functions (such as antiinflammation, anti-osteoclast, and osteogenesis) of biomaterials on periodontal tissue engineering, as it is easily obtained and accessible for observation (Eskan et al, 2012;Manilay and Zouali, 2014;Tsukasaki et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

A Simplified and Effective Method for Generation of Experimental Murine Periodontitis Model

Feng

Tang

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Periodontitis, a common disease that can lead to bone destruction, periodontal attachment loss, and tooth loss, is the major cause for oral tissue engineering. Experimental periodontitis is a suitable disease-model for studying bone regeneration and the potential therapeutic role of biomaterials on periodontal tissue engineering, as this in vivo model could be employed to mimic the natural host response under bacteria-caused oral pathological environments. Although large animals with ligature-induced periodontitis have mostly been used for experiments, a mouse model is a better choice for several reasons. Inserting ligature threads through the interproximal space between the teeth is the key step in establishing a periodontitis model, and it is easy to achieve in large animals, but difficult in mice due to the limited operating space. In this work, we provide a new and proven approach for periodontitis induction in mice using C+ nickel-titanium root canal files and stainless-steel ligature wires. The validity of this method was assessed by evaluating alveolar bone loss via micro-CT and detecting periodontal inflammation by histological staining and qPCR after the treatments. Progressive alveolar bone loss was observed from day 3 after the ligature-placement. Infiltration and accumulation of F4/80+ macrophage was also detected. In accordance with the histological results, there was upregulation of the expression levels of the inflammatory genes Il1β, Tnf-α, and Il6 in gingival tissues isolated from the ligation sites. Our results suggest that this novel method could resolve the difficulty of ligature-placement in mice and consequently contribute to further use of mouse models for studying the pathological mechanisms of periodontitis and developing potential periodontal tissue regeneration strategies. C+ files, which are made of nickel-titanium, are tough, elastic, and sufficiently thin to pass through the interproximal space between the teeth after pre-bending to form an appropriate angle, thus providing an access for ligature wire insertion. As a common tool in the dental clinic, it is familiar to researchers of oral biology, and can provide the feasibility for wide application of our method.

show abstract

Shockwave Therapy Efficiently Cures Multispecies Chronic Periodontitis in a Humanized Rat Model

Cited by 9 publications

References 38 publications

Antibiofilm efficacy of focused high-energy extracorporeal shockwaves and antibiotics in vitro

Antibiofilm efficacy of focused high-energy extracorporeal shockwaves and antibiotics in vitro

Relevance of Biofilm Models in Periodontal Research: From Static to Dynamic Systems

A Simplified and Effective Method for Generation of Experimental Murine Periodontitis Model

Contact Info

Product

Resources

About