Shock Waves Activate In Vitro Cultured Progenitors and Precursors Of Cardiac Cell Lineages from the Human Heart

Nurzynska, Daria; Meglio, Franca Di; Castaldo, Clotilde; Arcucci, Alessandro; Marlinghaus, Ernst; Russo, Sergio; Corrado, Bruno; Santo, Luca Salvatore De; Baldascino, Francesco; Cotrufo, Maurizio; Montagnani, Stefania

doi:10.1016/j.ultrasmedbio.2007.07.017

Cited by 59 publications

(32 citation statements)

References 19 publications

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“…Moreover, several hypotheses for Li-ESWT's mechanisms of action in erectile function recovery have been inspired by previous studies conducted in other basic science fields. It was thus shown that Li-ESWT triggers the activation of various intracellular signaling pathways causing upregulation of numerous angiogenic factors (i.e., VEGF…) to promote neovascularization and mobilizes the recruitment of endogenous progenitor cells thereby favoring angiogenesis and tissue regeneration in cardiovascular medicine and wound healing (17)(18)(19)(20)(21). These pro-angiogenic signaling pathways and recruitment of endogenous progenitor cells have also been shown to be activated in the penile tissue following Li-ESWT treatment in diabetic ED models (15,16), despite the fact that it does not upregulate the NO/cGMP pathway (14).…”

mentioning

confidence: 99%

Low-energy Shock Wave Therapy Ameliorates Erectile Dysfunction in a Pelvic Neurovascular Injuries Rat Model

Matheu

Sun

et al. 2016

The Journal of Sexual Medicine

113

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Introduction Erectile dysfunction (ED) caused by pelvic injuries is a common complication of civil and battlefield trauma with multiple neurovascular factors involved, and no effective therapeutic approach is available. Aims To test the effect and mechanisms of low-energy shock wave (LESW) therapy in a rat ED model induced by pelvic neurovascular injuries. Methods Thirty-two male Sprague-Dawley rats injected with 5-ethynyl-2′-deoxyuridine (EdU) at newborn were divided into 4 groups: sham surgery (Sham), pelvic neurovascular injury by bilateral cavernous nerve injury and internal pudendal bundle injury (PVNI), PVNI treated with LESW at low energy (Low), and PVNI treated with LESW at high energy (High). After LESW treatment, rats underwent erectile function measurement and the tissues were harvested for histologic and molecular study. To examine the effect of LESW on Schwann cells, in vitro studies were conducted. Main Outcome Measurements The intracavernous pressure (ICP) measurement, histological examination, and Western blot (WB) were conducted. Cell cycle, Schwann cell activation-related markers were examined in in vitro experiments. Results LESW treatment improves erectile function in a rat model of pelvic neurovascular injury by leading to angiogenesis, tissue restoration, and nerve generation with more endogenous EdU+ progenitor cells recruited to the damaged area and activation of Schwann cells. LESW facilitates more complete re-innervation of penile tissue with regeneration of neuronal nitric oxide synthase (nNOS)-positive nerves from the MPG to the penis. In vitro experiments demonstrated that LESW has a direct effect on Schwann cell proliferation. Schwann cell activation-related markers including p-Erk1/2 and p75 were upregulated after LESW treatment. Conclusion LESW-induced endogenous progenitor cell recruitment and Schwann cell activation coincides with angiogenesis, tissue, and nerve generation in a rat model of pelvic neurovascular injuries.

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mentioning

confidence: 99%

Low-energy Shock Wave Therapy Ameliorates Erectile Dysfunction in a Pelvic Neurovascular Injuries Rat Model

Matheu

Sun

et al. 2016

The Journal of Sexual Medicine

113

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“…It has been recently demonstrated that, in addition to angiogenesis due to migration and proliferation of endothelial cells in situ, EPCs contribute to neovascularization in ischemic tissue through a vasculogenetic mechanism and through secretion of a variety of angiogenic factors [18][19]. Finally, recent studies have marked out that ESW are able to recruit stem cells and to stimulate their differentiation in various damaged tissues inducing reparative phenomena [20][21][22].…”

Section: Discussionmentioning

confidence: 99%

Effects of shock wave therapy in the skin of patients with progressive systemic sclerosis: a pilot study

et al. 2010

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Results: After ESWT we observed a rapid and persistent reduction of RSS and decrease of VAS. There was no difference in skin thickness before and after ESWT; however we observed a more regular skin structure and an improvement in skin vascularization 90 days after treatment. EPCs and CECs increased 60 and 90 days after treatment, while serological biomarkers showed no variation before and after therapy. Conclusions: ESWT resulted in an improvement of VAS, RSS and of skin vascularscore. An increase of CECs and EPCs was also observed after therapy.

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“…In contrast to high-intensity extracorporeal shockwave therapy (ESWT) in the treatment of urolithiasis and other orthopaedic conditions, low-intensity ESWT has been shown to improve hemodynamic effects in many organs [88][89][90]. Several proposed mechanisms for the improvement in vascular supply and endothelial function include the induction of nonenzymatic production of physiologic amounts of NO [91], activation of intracellular signalling pathways, and increased expression of VEGF and its receptor, Flt-1, resulting in neovascularization [92,93].…”

Section: Extracorporeal Shockwave Therapymentioning

confidence: 99%

Emerging and Novel Therapeutic Approaches in the Treatment of Male Erectile Dysfunction

Chung

Brock

2011

Curr Urol Rep

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Over the past few decades, a dramatic increase in our understanding of the pathophysiology of penile erection has occurred. The advent of phosphodiesterase type 5 inhibitors has revolutionized the treatment of erectile dysfunction (ED), while the introduction of modern penile prosthesis has almost completely replaced the need for vascular surgery. Despite the efficacy of current therapeutic options for ED, they are not without limitations and remain insufficient to address the growing patient population and various underlying medical conditions. Newer and novel ED therapies aspire to treat underlying microvascular abnormalities, prevent cavernosal fibrosis, promote endothelial revascularization, modulate neurohormonal pathways, and regenerate new penile tissue.

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Shock Waves Activate In Vitro Cultured Progenitors and Precursors Of Cardiac Cell Lineages from the Human Heart

Cited by 59 publications

References 19 publications

Low-energy Shock Wave Therapy Ameliorates Erectile Dysfunction in a Pelvic Neurovascular Injuries Rat Model

Low-energy Shock Wave Therapy Ameliorates Erectile Dysfunction in a Pelvic Neurovascular Injuries Rat Model

Effects of shock wave therapy in the skin of patients with progressive systemic sclerosis: a pilot study

Emerging and Novel Therapeutic Approaches in the Treatment of Male Erectile Dysfunction

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