Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts

Bolívar, Juan M.; Consolati, Tanja; Mayr, Torsten; Nidetzky, Bernd

doi:10.1016/j.tibtech.2013.01.004

Cited by 59 publications

(100 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We use intrinsic here to indicate the absence of effects of the heterogeneous environment on the observable enzymatic reaction rate. The possibility of development of concentration gradients inside the porous support and the consequent impact on enzyme behavior need to be kept in mind however [10,15,19,27]. Effective concentrations inside the support, including that of the proton, can be dramatically different from the corresponding concentrations in bulk [27][28][29] (Scheme 1a).…”

Section: Enzyme Loading In High Capacity and High Quality For Immobilmentioning

confidence: 99%

“…Dynamic response of the heterogeneous environment to changes in the bulk phase is also of interest [4,10,23,139]. External diffusion can be controlled by the fluid dynamics at the reactor level [23,27,139]. By contrast, internal diffusion effects are often pronounced and cannot be readily controlled by an external variable since they depend on structural, hence internal features of the support [10,12].…”

Section: In-operando Internal Sensing In Heterogeneous Biocatalystsmentioning

confidence: 99%

See 1 more Smart Citation

Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

2016

Self Cite

View full text Add to dashboard Cite

Section: Enzyme Loading In High Capacity and High Quality For Immobilmentioning

confidence: 99%

Section: In-operando Internal Sensing In Heterogeneous Biocatalystsmentioning

confidence: 99%

Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

2016

Self Cite

View full text Add to dashboard Cite

“…56,60 However, since chemical reaction and diffusion are intrinsically coupled events in transformations catalyzed by solid enzyme immobilizates, η is not independent of E bound and the dependence generally starts to show at high enzyme loadings 7,[14][15][16] . Material properties thus become important in the effort of enhancing the degree of uncoupling of η from E bound .…”

Section: Heterogeneous O 2 -Dependent Biocatalysis Designed For Efficmentioning

confidence: 99%

Oriented Coimmobilization of Oxidase and Catalase on Tailor-Made Ordered Mesoporous Silica

et al. 2017

Self Cite

View full text Add to dashboard Cite

Mesoporous silica materials are promising carriers for enzyme immobilization in heterogeneous biocatalysis applications. By tailoring their pore structural framework, these materials are designable for appropriate enzyme binding capacity and internal diffusivity. To supply O 2 efficiently to solid-supported immobilized enzymes represents a core problem of heterogeneously catalyzed oxidative biotransformations. In this study, therefore, we synthesized and compared three internally well-ordered and two amorphous silica materials as enzyme carriers, each of those with pore sizes of ≥10 nm, to enable the co-immobilization of D-aminoacid oxidase (93 kDa) and catalase (245 kDa). Both enzymes were fused to the silica-binding module Z basic2 to facilitate their selective and oriented immobilization directly from crude protein mixtures on native silica materials. Analyzing the effects of varied pore architecture and internal surface area on the performance of the immobilized bienzymatic system, we showed that a uniform pore structural framework was beneficial for enzyme loading (≥ 70 mg protein/g carrier), immobilization yield (≥ 90%), surface and pore volume filling without hindered adsorption, and catalytic effectiveness (≥ 60%) of the co-immobilizate. Using the best carrier LP-SBA-15, we obtained a solid oxidase-catalase preparation with an activity of 2000 µmol/(min g_material) that was recyclable and stable during oxidation of D-methionine. These results affirm a strategy of optimizing immobilized O 2 -dependent enzymes via tunable internal structuring of the silica material used as carrier. They thus make a significant advance towards the molecular design of heterogeneous oxidation biocatalysts on mesoporous silica supports.

show abstract

“…Although different immobilization techniques can lead to good results, some problems remain in the characterization of the best operative conditions, depending both on the physical effect of mass transfer and the insufficient development of analytical methods [132]. However, new and interesting systems have been proposed for the in operando characterization of biomicroreactors, such as opto-chemical sensors for pH and O 2 measurements based on the co-immobilization of luminescent dyes in the microreactor [132], or the so-called "luminescent multiple optical chemical sensors" [133].…”

Section: Magnetic Beadsmentioning

confidence: 99%

“…However, new and interesting systems have been proposed for the in operando characterization of biomicroreactors, such as opto-chemical sensors for pH and O 2 measurements based on the co-immobilization of luminescent dyes in the microreactor [132], or the so-called "luminescent multiple optical chemical sensors" [133].…”

Section: Magnetic Beadsmentioning

confidence: 99%

Enzymatic microreactors in biocatalysis: history, features, and future perspectives

Laurenti¹,

Vianna²

2016

Biocatalysis

View full text Add to dashboard Cite

Microfluidic reaction devices are a very promising technology for chemical and biochemical processes. In microreactors, the micro dimensions, coupled with a high surface area/volume ratio, permit rapid heat exchange and mass transfer, resulting in higher reaction yields and reaction rates than in conventional reactors. Moreover, the lower energy consumption and easier separation of products permit these systems to have a lower environmental impact compared to macroscale, conventional reactors. Due to these benefits, the use of microreactors is increasing in the biocatalysis field, both by using enzymes in solution and their immobilized counterparts. Following an introduction to the most common applications of microreactors in chemical processes, a broad overview will be given of the latest applications in biocatalytic processes performed in microreactors with free or immobilized enzymes. In particular, attention is given to the nature of the materials used as a support for the enzymes and the strategies employed for their immobilization. Mathematical and engineering aspects concerning fluid dynamics in microreactors were also taken into account as fundamental factors for the optimization of these systems.

show abstract

Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts

Cited by 59 publications

References 90 publications

Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

Oriented Coimmobilization of Oxidase and Catalase on Tailor-Made Ordered Mesoporous Silica

Enzymatic microreactors in biocatalysis: history, features, and future perspectives

Contact Info

Product

Resources

About