Shikonin promotes rat periodontal bone defect repair and osteogenic differentiation of BMSCs by p38 MAPK pathway

Lin, Xi; Wang, Yuxin; Guo, Xin; Li, Chen; Wu, Kuaifeng; Wang, Siya; Yu, Xijiao; Du, Yi

doi:10.1007/s10266-022-00774-w

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…It is well known that the p38/MAPK pathway regulates the cell proliferation and osteogenic differentiation of BMSCs [ 19 , 20 ], and it is an important downstream effector pathway for FNDC5 [ 21 , 22 ]. Therefore, to determine whether miR-150-5p regulates osteogenic differentiation of BMSCs through the FNDC5/p38/MAPK signaling axis, we determined the expression of p38 and p-p38 protein using western blot.…”

Section: Resultsmentioning

confidence: 99%

mir-150-5p inhibits the osteogenic differentiation of bone marrow-derived mesenchymal stem cells by targeting irisin to regulate the p38/MAPK signaling pathway

Qi,

Zhang,

Dong

et al. 2024

J Orthop Surg Res

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Purpose To study the effect of miR-150-5p on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs), and further explore the relationship between its regulatory mechanism and irisin. Methods We isolated mouse BMSCs, and induced osteogenic differentiation by osteogenic induction medium. Using qPCR to detect the expression of osteogenic differentiation-related genes, western blot to detect the expression of osteogenic differentiation-related proteins, and luciferase reporter system to verify that FNDC5 is the target of miR-150-5p. Irisin intraperitoneal injection to treat osteoporosis in mice constructed by subcutaneous injection of dexamethasone. Results Up-regulation of miR-150-5p inhibited the proliferation of BMSCs, and decreased the content of osteocalcin, ALP activity, calcium deposition, the expression of osteogenic differentiation genes (Runx2, OSX, OCN, OPN, ALP and BMP2) and protein (BMP2, OCN, and Runx2). And down-regulation of miR-150-5p plays the opposite role of up-regulation of miR-150-5p on osteogenic differentiation of BMSCs. Results of luciferase reporter gene assay showed that FNDC5 gene was the target gene of miR-150-5p, and miR-150-5p inhibited the expression of FNDC5 in mouse BMSCs. The expression of osteogenic differentiation genes and protein, the content of osteocalcin, ALP activity and calcium deposition in BMSCs co-overexpressed by miR-150-5p and FNDC5 was significantly higher than that of miR-150-5p overexpressed alone. In addition, the overexpression of FNDC5 reversed the blocked of p38/MAPK pathway by the overexpression of miR-150-5p in BMSCs. Irisin, a protein encoded by FNDC5 gene, improved symptoms in osteoporosis mice through intraperitoneal injection, while the inhibitor of p38/MAPK pathway weakened this function of irisin. Conclusion miR-150-5p inhibits the osteogenic differentiation of BMSCs by targeting irisin to regulate the/p38/MAPK signaling pathway, and miR-150-5p/irisin/p38 pathway is a potential target for treating osteoporosis.

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Section: Resultsmentioning

confidence: 99%

mir-150-5p inhibits the osteogenic differentiation of bone marrow-derived mesenchymal stem cells by targeting irisin to regulate the p38/MAPK signaling pathway

Qi,

Zhang,

Dong

et al. 2024

J Orthop Surg Res

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“…On the other hand, the same pathway may play opposite roles during cell differentiation under different microenvironment signals. Pharmacological antagonism of the p38 MAPK pathway can effectively inhibit the tissue destruction process of periodontitis and promote bone regeneration ( 46 , 47 ). In a word, as the MAPK pathway can regulate the regeneration of periodontium, the exploration of its key targets and corresponding stimulating factors is of great significance.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome landscape comparison of periodontium in developmental and renewal stages

Deng

Luo²,

Xie³

et al. 2023

Front. Endocrinol.

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ObjectivesPeriodontium regeneration remains a significant challenge in clinics and research, and it is essential to understand the stage-specific biological process in situ. However, differing findings have been reported, and the mechanism has yet to be elucidated. The periodontium of adult mice molars is considered to be stable remodeling tissue. At the same time, the continuously growing incisors and the developing dental follicle (DF) of postnatal mice highly represent fast remodeling tissue. In this study, we attempted to explore different clues of temporal and spatial comparisons to provide improved references for periodontal regeneration.MethodsPeriodontal tissues from the developing periodontium (DeP) of postnatal mice, and continuously growing periodontium (CgP) and stable remodeling periodontium (ReP) of adult mice were isolated and compared using RNA sequencing. Based on the Dep and CgP separately compared with the ReP, differentially expressed genes and signaling pathways were analyzed using GO, KEGG databases, and Ingenuity Pathway Analysis (IPA). The results and validation were obtained by immunofluorescence staining and RT-PCR assays. Data were expressed as means ± standard deviation (SD) and analyzed by GraphPad Prism 8 software package, and one-way ANOVA was used to test multiple groups.ResultsPrincipal component analysis showed that the three groups of periodontal tissue were successfully isolated and had distinct expression profiles. A total of 792 and 612 DEGs were identified in the DeP and CgP groups compared with the ReP. Upregulated DEGs in the DeP were closely related to developmental processes, while the CgP showed significantly enhanced cellular energy metabolism. The DeP and CgP showed a common downregulation of the immune response, with activation, migration, and recruitment of immune cells. IPA and further validation jointly suggested that the MyD88/p38 MAPK pathway played an essential regulatory role in periodontium remodeling.ConclusionTissue development, energy metabolism, and immune response were critical regulatory processes during periodontal remodeling. Developmental and adult stages of periodontal remodeling showed different expression patterns. These results contribute to a deeper understanding of periodontal development and remodeling and may provide references for periodontal regeneration.

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Inhibition of PKM2 suppresses osteoclastogenesis and alleviates bone loss in mouse periodontitis

Li,

Liu,

et al. 2024

International Immunopharmacology

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Shikonin promotes rat periodontal bone defect repair and osteogenic differentiation of BMSCs by p38 MAPK pathway

Cited by 5 publications

References 39 publications

mir-150-5p inhibits the osteogenic differentiation of bone marrow-derived mesenchymal stem cells by targeting irisin to regulate the p38/MAPK signaling pathway

mir-150-5p inhibits the osteogenic differentiation of bone marrow-derived mesenchymal stem cells by targeting irisin to regulate the p38/MAPK signaling pathway

Transcriptome landscape comparison of periodontium in developmental and renewal stages

Inhibition of PKM2 suppresses osteoclastogenesis and alleviates bone loss in mouse periodontitis

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