Shikonin inhibits maturation of bone marrow‐derived dendritic cells and suppresses allergic airway inflammation in a murine model of asthma

Abstract

BACKGROUND AND PURPOSEShikonin exhibits a wide range of anti-inflammatory actions. Here, we assessed its effects on maturation of murine bone marrow-derived dendritic cells (BM-DCs) and on allergic reactions in a murine model of asthma. EXPERIMENTAL APPROACHCultured murine BM-DCs were used to investigate the effects of shikonin on expression of cell surface markers and their stimulation of T-cell proliferation and cytokine production. The therapeutic potential of shikonin was evaluated in a model of allergic airway disease. KEY RESULTSShikonin dose-dependently inhibited expression of major histocompatibility complex class II, CD80, CD86, CCR7 and OX40L on BM-DCs, induced by a mixture of ovalbumin (OVA; 100 mg·mL CONCLUSION AND IMPLICATIONSShikonin effectively suppressed OVA + TSLP-induced BM-DC maturation in vitro and inhibited allergic inflammation and airway hyperresponsiveness in a murine model of asthma, showing good potential as a treatment for allergic asthma. Also, our model provides a novel platform for screening drugs for allergic diseases.

Inhibition of high-mobility group box 1 in lung reduced airway inflammation and remodeling in a mouse model of chronic asthma

“…Mice were given an aerosol challenge of 5 mL 4% OVA in PBS once a day, 5 days per week, for 4 weeks. On days 29,31,33,36,38,40,43,45,47,50,52, and 54, mice were given an intratracheal instillation of PBS (OVA group), with or without anti-HMGB1 neutralizing antibody (30 mg/mouse; OVA + anti-HMGB1 Ab group), or a control rabbit IgG antibody (Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) (30 mg/mouse; OVA + isotype control Ab group) 1 h before OVA exposure. There were 8-10 mice per group.…”

“…The dyes and related solutions purchased from Sigma. Quantification of airway inflammation, PAS-positive bronchial epithelial cells, and collagen fibril deposition were performed according to methods reported in a previous study [31,32]. For immunohistochemistry, after deparaffinization, lung sections were hydrated, and epitope retrieval was performed according to the manufacturer's protocol (DakoCytomation Target Retrieval Solution, pH 6.0; DakoCytomation, Carpinteria, CA, USA).…”

“…Mice serum samples were collected on day 30 and the anti-OVA immunoglobulin (Ig)E antibody titer was determined by ELISA, as previously described [31].…”

Molecular Imaging of Heparan Sulfate Expression with Radiolabeled Recombinant Eosinophil Cationic Protein Predicts Allergic Lung Inflammation in a Mouse Model for Asthma

“…These asthma mice exhibited airway hyperresponsiveness and inflammation, as shown in previous studies (16). 123 I-rECP was injected into either asthma or normal mice for analysis by both SPECT/CT imaging and biodistribution in lung tissues.…”

Effect of shikonin on multidrug resistance in HepG2: The role of SIRT1

“…(Boraginaceae) (Brigham et al, 1999;Yang et al, 2009). Shikonin and its analogues are potential pharmaceutical agents with anticancer activities in addition to anti-inflammatory properties (Lee et al, 2010;Wang et al, 2013;Yao & Zhou., 2010). Shikonin preferentially inhibits the growth of human epidermoid carcinoma cells in a concentration-and time-dependent manner (Singh et al, 2003).…”