Shikimate Induced Transcriptional Activation of Protocatechuate Biosynthesis Genes by QuiR, a LysR-Type Transcriptional Regulator, in Listeria monocytogenes

Prezioso, Stephanie M.; Xue, Kevin; Leung, Nelly; Gray-Owen, Scott D.; Christendat, Dinesh

doi:10.1016/j.jmb.2018.03.003

Cited by 8 publications

(17 citation statements)

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“…Although it has been proposed that the genes from both operons contribute to protocatechuate biosynthesis, not all of the enzymes for the pathway have been identified. Bioinformatics analyses using the protein sequences encoded by the genes within the two operons identified Lmo2234 as a DSD enzyme catalysing the final step in protocatechuate biosynthesis in Listeria spp (Quistgaard et al, 2016;Prezioso et al, 2018). Therefore, detailed bioinformatics, biochemical and structural analyses were undertaken to establish the role of Lmo2234 in Listeria monocytogenes.…”

Section: The Identification Of Quic2 (Lmo2234)mentioning

confidence: 99%

“…The core enzymes necessary for protocatechuate biosynthesis are shikimate and quinate dehydrogenases (SDH, EC 1.1.1.24; QDH, EC 1.1.1.25), dehydroquinate dehydratase (DHQD, EC 1.1.1.282), and dehydroshikimate dehydratase (DSD, EC 4.2.1.118) (Teramoto et al, 2009). The identification and characterization of this core set of enzymes with the exception of DSD have been reported for L. monocytogenes and L. innocua (Prezioso et al, 2018). In addition, an atypical shikimate dehydrogenase (SdhD) was also found, which redirects shikimate to dehydroshikimate for subsequent conversion to protocatechuate (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…The genes from these operons encode proteins/enzymes associated with the 'quinate/ shikimate' utilization pathway ( Fig. 1A and B) (Prezioso et al, 2018). The typical quinate/shikimate utilization pathway is divided into two: firstly, quinate and shikimate are diverted in the protocatechuate biosynthetic pathway to produce protocatechuate, and secondly, protocatechuate is shuttled through the β-ketoadipate pathway to produce intermediates of the TCA cycle ( Fig.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, an atypical shikimate dehydrogenase (SdhD) was also found, which redirects shikimate to dehydroshikimate for subsequent conversion to protocatechuate (Fig. 1B) (Prezioso et al, 2018). In contrast to other soil borne microorganisms, Listeria spp.…”

Section: Introductionmentioning

confidence: 99%

“…In contrast to other soil borne microorganisms, Listeria spp. lack the downstream β-ketoadipate pathway required for shuttling aromatic compounds from the environment to the tricarboxylic acid (TCA) cycle (Prezioso et al, 2018). The absence of the β-ketoadipate pathway in Listeria spp.…”

Section: Introductionmentioning

confidence: 99%

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QuiC2 represents a functionally distinct class of dehydroshikimate dehydratases identified in Listeria species including Listeria monocytogenes

Xue

Prezioso

Christendat

2020

Environmental Microbiology

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Summary Many Listeria species including L. monocytogenes contain the pathway for the biosynthesis of protocatechuate from shikimate and quinate. The qui1 and qui2 operons within these Listeria spp. encode enzymes for this pathway. The diversion of shikimate pathway intermediates in some Listeria species to produce protocatechuate suggests an important biological role for this compound to these organisms. A total of seven ORFs, including quiC2, were identified within qui1 and qui2, however only three proteins encoded by the operons have been functionally annotated. The final step in Listeria's protocatechuate biosynthesis involves the conversion of dehydroshikimate by a dehydroshikimate dehydratase (DSD). In this study, we demonstrate that QuiC2 functions as a DSD in Listeria spp. through biochemical and structural analyses. Moreover, we show that QuiC2 forms a phylogenetic cluster distinct from other functionally annotated DSDs. The individual phylogenetic clusters of DSD are represented by enzymes that produce protocatechuate for distinct biological processes. Similarly, QuiC2 is expected to produce protocatechuate for a novel biological process. We postulate that protocatechuate produced by DSDs found within the QuiC2 phylogenetic cluster provides an ecological niche for representative organisms.

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Section: The Identification Of Quic2 (Lmo2234)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

QuiC2 represents a functionally distinct class of dehydroshikimate dehydratases identified in Listeria species including Listeria monocytogenes

Xue

Prezioso

Christendat

2020

Environmental Microbiology

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A novel LysR‐type regulator negatively affects biosynthesis of the immunosuppressant brasilicardin

Wolański

Krawiec

Schwarz

et al. 2020

Engineering in Life Sciences

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Brasilicardin A (BraA) is a promising immunosuppressive compound produced naturally by the pathogenic bacterium Nocardia terpenica IFM 0406. Heterologous host expression of brasilicardin gene cluster showed to be efficient to bypass the safety issues, low production levels and lack of genetic tools related with the use of native producer. Further improvement of production yields requires better understanding of gene expression regulation within the BraA biosynthetic gene cluster (Bra‐BGC); however, the only so far known regulator of this gene cluster is Bra12. In this study, we discovered the protein LysRNt, a novel member of the LysR‐type transcriptional regulator family, as a regulator of the Bra‐BGC. Using in vitro approaches, we identified the gene promoters which are controlled by LysRNt within the Bra‐BGC. Corresponding genes encode enzymes involved in BraA biosynthesis as well as the key Bra‐BGC regulator Bra12. Importantly, we provide in vivo evidence that LysRNt negatively affects production of brasilicardin congeners in the heterologous host Amycolatopsis japonicum. Finally, we demonstrate that some of the pathway related metabolites, and their chemical analogs, can interact with LysRNt which in turn affects its DNA‐binding activity.

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Fundamentals and Exceptions of the LysR-type Transcriptional Regulators

Demeester,

De Paepe,

De Mey

2024

ACS Synth. Biol.

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LysR-type transcriptional regulators (LTTRs) are emerging as a promising group of macromolecules for the field of biosensors. As the largest family of bacterial transcription factors, the LTTRs represent a vast and mostly untapped repertoire of sensor proteins. To fully harness these regulators for transcription factor-based biosensor development, it is crucial to understand their underlying mechanisms and functionalities. In the first part, this Review discusses the established model and features of LTTRs. As dual-function regulators, these inducible transcription factors exude precise control over their regulatory targets. In the second part of this Review, an overview is given of the exceptions to the "classic" LTTR model. While a general regulatory mechanism has helped elucidate the intricate regulation performed by LTTRs, it is essential to recognize the variations within the family. By combining this knowledge, characterization of new regulators can be done more efficiently and accurately, accelerating the expansion of transcriptional sensors for biosensor development. Unlocking the pool of LTTRs would significantly expand the currently limited range of detectable molecules and regulatory functions available for the implementation of novel synthetic genetic circuitry.

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Shikimate Induced Transcriptional Activation of Protocatechuate Biosynthesis Genes by QuiR, a LysR-Type Transcriptional Regulator, in Listeria monocytogenes

Cited by 8 publications

References 50 publications

QuiC2 represents a functionally distinct class of dehydroshikimate dehydratases identified in Listeria species including Listeria monocytogenes

QuiC2 represents a functionally distinct class of dehydroshikimate dehydratases identified in Listeria species including Listeria monocytogenes

A novel LysR‐type regulator negatively affects biosynthesis of the immunosuppressant brasilicardin

Fundamentals and Exceptions of the LysR-type Transcriptional Regulators

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