Shigella flexneri Disruption of Cellular Tension Promotes Intercellular Spread

Duncan-Lowey, Jeffrey K.; Wiscovitch, Alexandra L.; Wood, Thomas E.; Goldberg, Marcia B.; Russo, Brian C.

doi:10.1016/j.celrep.2020.108409

Cited by 26 publications

(22 citation statements)

References 63 publications

(87 reference statements)

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“…Collectively, these findings suggest that cortical tension at tight junctions presents a barrier that L. monocytogenes must overcome in order to spread efficiently in tissues. Remarkably, this strategy of relieving cortical tension to promote intercellular spread is shared by the evolutionarily unrelated bacteria Rickettsia parkeri and Shigella flexneri (Dowd et al., 2020b; Duncan‐Lowey et al., 2020; Lamason et al., 2016), underscoring the idea that tension at cell‐cell junctions is an obstacle that microbes circumvent to establish infection.…”

Section: Inlc Promotes Cell‐to‐cell Spread Of Bacteriamentioning

confidence: 99%

Role of internalin proteins in the pathogenesis of Listeria monocytogenes

Ireton

Mortuza

Gyanwali

et al. 2021

Molecular Microbiology

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Listeria monocytogenes is a food‐borne bacterium that causes gastroenteritis, meningitis, or abortion. L. monocytogenes induces its internalization (entry) into human cells and either spreads laterally in tissues or transcytoses to traverse anatomical barriers. In this review, we discuss mechanisms by which five structurally related proteins of the “internalin” family of L. monocytogenes (InlA, InlB, InlC, InlF, and InlP) interact with distinct host receptors to promote infection of human cells and/or crossing of the intestinal, blood–brain, or placental barriers. We focus on recent results demonstrating that the internalin proteins InlA, InlB, and InlC exploit exocytic pathways to stimulate transcytosis, entry, or cell‐to‐cell spread, respectively. We also discuss evidence that InlA‐mediated transcytosis contributes to traversal of the intestinal barrier, whereas InlF promotes entry into endothelial cells to breach the blood–brain barrier. InlB also facilitates the crossing of the blood–brain barrier, but does so by extending the longevity of infected monocytes that may subsequently act as a “Trojan horse” to transfer bacteria to the brain. InlA, InlB, and InlP each contribute to fetoplacental infection by targeting syncytiotrophoblast or cytotrophoblast layers of the placenta. This work highlights the diverse functions of internalins and the complex mechanisms by which these structurally related proteins contribute to disease.

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Section: Inlc Promotes Cell‐to‐cell Spread Of Bacteriamentioning

confidence: 99%

Role of internalin proteins in the pathogenesis of Listeria monocytogenes

Ireton

Mortuza

Gyanwali

et al. 2021

Molecular Microbiology

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“…These results suggest that one or more T3SS effector stimulates exocytosis, perhaps through interaction with exocyst components and/or with host regulators of this complex. Although bacterial proteins that induce exocytosis are currently unknown, one candidate is IpaC which contributes to membrane pore formation by the T3SS (Mattock & Blocker, 2017) and also promotes Shigella protrusion formation in HeLa and Caco-2 cells (Duncan-Lowey et al, 2020).…”

Section: The Exocyst Vamp3 and Rala Promote Shigella Protrusion Formation And Spread In The Polarized Epithelial Cell Caco-2 Bbe1mentioning

confidence: 99%

“…This myosin protein contains separate domains that bind F-actin and phospholipids (Kerber & Cheney, 2011), and may control the structure of Shigella protrusions by linking actin tails to the plasma membrane (Bishai et al, 2013;Dowd, Mortuza, & Ireton, 2020). Finally, recent results suggest that the efficient generation of Shigella protrusions requires relief of plasma membrane tension at intercellular junctions in epithelia (Duncan-Lowey et al, 2020). Specifically, the T3SS component IpaC interacts with the host adherens junction protein β-catenin, resulting in diminished intercellular tension that would otherwise oppose protrusion formation (Dowd, Mortuza, & Ireton, 2020;Duncan-Lowey et al, 2020).…”

mentioning

confidence: 99%

“…Finally, recent results suggest that the efficient generation of Shigella protrusions requires relief of plasma membrane tension at intercellular junctions in epithelia (Duncan-Lowey et al, 2020). Specifically, the T3SS component IpaC interacts with the host adherens junction protein β-catenin, resulting in diminished intercellular tension that would otherwise oppose protrusion formation (Dowd, Mortuza, & Ireton, 2020;Duncan-Lowey et al, 2020). Despite the recent advances described above, the mechanism of protrusion formation during cell-to-cell spread Shigella remains incompletely understood.…”

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confidence: 99%

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Shigella flexneri subverts host polarized exocytosis to enhance cell‐to‐cell spread

Herath

Roy

Gianfelice

et al. 2021

Molecular Microbiology

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“…Shigella flexneri is known as a human pathogen infecting the colonic epithelial cells. With reduction of intercellular tension is able to spread between cells [66].…”

Section: Occurrence Of Allochthonous Microorganisms In Traditionally Processed Cheesementioning

confidence: 99%

Microbial Diversity of Traditionally Processed Cheese from Northeastern Region of Transylvania (Romania)

György¹,

Laslo²

2021

Fermentation - Processes, Benefits and Risks

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The composition and production technology of the cheese are extremely diverse. There are a wide variety of microbial species on their surface, with a much smaller number inside of the product. The microbiota of the cheese may be composed of beneficial microorganisms, spoilage and foodborne pathogens. Identification and characterization of the microorganisms present in these products are important nutrition, food safety and technological aspects. During our work we evaluated the prevalence of allochthonous bacteria and microscopic fungi in traditionally processed cheeses from northeastern region of Transylvania, with classical microbiological culture methods. Based on the results the microbiota of the analysed cheeses was highly diversified. The identified bacteria with the highest prevalence from different selective media, were as follows: Escherichia coli, Enterococcus durans, Enterococcus faecalis, Shigella flexnerii, Proteus vulgaris, Stenotrophomonas maltophilia, Staphylococcus equorum subsp. equorum, Staphylococcus equorum subsp. linens, Halomonas alkaliphila, Kocuria rhizophila, Hafnia paralvei, Bacillus licheniformis and Klebsiella michiganensis.

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Shigella flexneri Disruption of Cellular Tension Promotes Intercellular Spread

Abstract: Highlights d Shigella flexneri reduces membrane tension to promote intercellular spread d The type 3 secretion protein IpaC is required for efficient protrusion formation d IpaC interacts with b-catenin, altering the dynamics of adherens junctions

Cited by 26 publications

References 63 publications

Role of internalin proteins in the pathogenesis of Listeria monocytogenes

Role of internalin proteins in the pathogenesis of Listeria monocytogenes

Shigella flexneri subverts host polarized exocytosis to enhance cell‐to‐cell spread

Microbial Diversity of Traditionally Processed Cheese from Northeastern Region of Transylvania (Romania)

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