Shigella and Escherichia coli Strategies for Survival at Low pH

Small, P. L. C.

doi:10.7883/yoken1952.51.supplement1_s81

Cited by 6 publications

(2 citation statements)

References 24 publications

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“…coli is an acid-resistant enteric micro-organism capable of withstanding extreme acid exposure for several hours (Small et al, 1994;Small, 1998). AR2, the glutamatedependent system, is the most robust of the four systems that protect E. coli from acid stresses encountered in the environment and in the stomach of the host (CastanieCornet et al, 1999;Iyer et al, 2003;Price et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Sodium regulates Escherichia coli acid resistance, and influences GadX- and GadW-dependent activation of gadE

Richard

Foster

2007

Microbiology

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Section: Discussionmentioning

confidence: 99%

Sodium regulates Escherichia coli acid resistance, and influences GadX- and GadW-dependent activation of gadE

Richard

Foster

2007

Microbiology

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“…First, stool culture has limited sensitivity for detecting bacterial enteropathogens, including Shigella , and takes 2–3 days [ 8 ]. Culture is also challenging as Shigella requires stringent sampling, transport, and growth conditions for optimal recovery and can also be sensitive to changes in pH [ 9 ], temperature, and oxygen levels [ 10 ]. Delayed placement in transport media, prolonged transport times, and unstable cold chain may compromise the yield.…”

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confidence: 99%

Shigella Detection and Molecular Serotyping With a Customized TaqMan Array Card in the Enterics for Global Health (EFGH): Shigella Surveillance Study

Liu,

Garcia Bardales,

Islam

et al. 2024

Open Forum Infectious Diseases

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Background Quantitative polymerase chain reaction (qPCR) targeting ipaH has been proven to be highly efficient in detecting Shigella in clinical samples compared to culture-based methods, which underestimate Shigella burden by 2- to 3-fold. qPCR assays have also been developed for Shigella speciation and serotyping, which is critical for both vaccine development and evaluation. Methods The Enterics for Global Health (EFGH) Shigella surveillance study will utilize a customized real-time PCR–based TaqMan Array Card (TAC) interrogating 82 targets, for the detection and differentiation of Shigella spp, Shigella sonnei, Shigella flexneri serotypes, other diarrhea-associated enteropathogens, and antimicrobial resistance (AMR) genes. Total nucleic acid will be extracted from rectal swabs or stool samples, and assayed on TAC. Quantitative analysis will be performed to determine the likely attribution of Shigella and other particular etiologies of diarrhea using the quantification cycle cutoffs derived from previous studies. The qPCR results will be compared to conventional culture, serotyping, and phenotypic susceptibility approaches in EFGH. Conclusions TAC enables simultaneous detection of diarrheal etiologies, the principal pathogen subtypes, and AMR genes. The high sensitivity of the assay enables more accurate estimation of Shigella-attributed disease burden, which is critical to informing policy and in the design of future clinical trials.

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Superficial gastro-intestinal infectionsA clinical overview

Keusch

2002

Molecular Medical Microbiology

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Shigella and Escherichia coli Strategies for Survival at Low pH

Cited by 6 publications

References 24 publications

Sodium regulates Escherichia coli acid resistance, and influences GadX- and GadW-dependent activation of gadE

Sodium regulates Escherichia coli acid resistance, and influences GadX- and GadW-dependent activation of gadE

Shigella Detection and Molecular Serotyping With a Customized TaqMan Array Card in the Enterics for Global Health (EFGH): Shigella Surveillance Study

Superficial gastro-intestinal infectionsA clinical overview

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