Shiga toxin–producing Escherichia coli in British Columbia, 2011–2017: Analysis to inform exclusion guidelines

Noftall, K; Taylor, Marsha; Hoang, Linda; Galanis, Eleni

doi:10.14745/ccdr.v45i09a03

Cited by 10 publications

(8 citation statements)

References 31 publications

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“…O157:H7 STEC is the most common serotype in many locations; however, the non-O157 group often outnumbers O157 in clinical specimens when considered in their entirety [22]. This, coupled with the observed decline in O157 incidence in many locations, is demonstrative of the significant clinical burden of non-O157 and the importance of implementing sensitive methods for their detection [21,23,24]. Our study captured 729 cases of non-O157 STEC over a period of 4 years.…”

Section: Discussionmentioning

confidence: 99%

“…Data from Korea showed O103, O26, O91 and O8 [19] and Japan reported O26, O111, O103, O121 and O145 [20] as their predominant serogroups. British Columbia, Canada (located west of Alberta) found their top serogroups to be O121, O26, O103, O117 and O111 [21]. Internationally, and within Canada, there is considerable variation in the most common serogroups.…”

Section: Discussionmentioning

confidence: 99%

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Epidemiology of Non-O157 Shiga Toxin-Producing Escherichia coli in the Province of Alberta, Canada, from 2018 to 2021

2022

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Non-O157 serogroups contribute significantly to the burden of disease caused by Shiga toxin-producing Escherichia coli (STEC) and have been underrecognized by traditional detection algorithms. We described the epidemiology of non-O157 STEC in Alberta, Canada for the period of 2018 to 2021. All non-O157 STEC isolated from clinical samples were submitted for serotyping and qPCR targeting the stx1 and stx2 genes. A total of 729 isolates were identified. Increased detection occurred over the summer months, peaking in July. Patients 18 years and younger made up 42.4% of cases, with 31.1% in those 0–9 years of age. There was a slight female predominance (399/729, 54.7%) A total of 50 different serogroups were detected; the most common were O26 (30.3%), O103 (15.9%), O111 (12.8%), O121 (11.0%), O118 (3.3%) and O71 (2.9%). These six serogroups made up 76.2% of all isolates. In total, 567 (77.8%) were positive for stx1, 114 (15.6%) were positive for stx2 and 48 (6.6%) were positive for both stx1 and stx2. A wide variety of non-O157 serogroups have been detected in Alberta, with the most frequent serogroups differing from other locations. These results highlight the need for further characterization of their virulence factors and clinical impact.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Epidemiology of Non-O157 Shiga Toxin-Producing Escherichia coli in the Province of Alberta, Canada, from 2018 to 2021

2022

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“…( 5) Swift identification of infected individuals and potential contacts. (6) Implementation of control measures endorsed by British guidelines [6] . A holistic approach involving health professionals, medical institutions, and the community, is essential.…”

Section: (3) Livestock Vaccination With Agents Like Epitopix Andmentioning

confidence: 99%

“…Key transmission routes include contaminated food, infected persons, and both direct and indirect transmission 4 . Prevention methods include: Strict hand and food hygiene. Thorough food heating to neutralize STEC (Shiga toxin-producing E. coli ) 5 . Livestock vaccination with agents like Epitopix and Econiche 4 . Improved hygiene in meat processing and enhanced farming techniques. Swift identification of infected individuals and potential contacts. Implementation of control measures endorsed by British guidelines 6 . …”

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confidence: 99%

Outbreak investigation and implications: assessing the severity of E. coli outbreak in Alberta, pediatric hospitalizations, and the rise of hemolytic uremic syndrome

Ashinze,

Banerjee,

Ademola

et al. 2024

International Journal of Surgery: Global Health

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We pen this communique with the intent of emphasizing an emergent and deeply concerning matter: the outbreak of Escherichia coli in Alberta, Canada, with a particular focus on its profound ramifications for pediatric populations, evidenced by the concomitant rise in hemolytic uremic syndrome (HUS) occurrences.The recent E. coli outbreak in Alberta, Canada, has raised serious concerns, particularly regarding its impact on pediatric populations. The surge in HUS highlights the severity of the situation. This paper underscores the urgency of effective preventive strategies and a coordinated response.E. coli, typically found in the intestines of animals and humans, is predominantly benign. However, specific strains can lead to severe health implications [1] . The recent epidemic in Alberta has seen a notable increase in E. coli infections, disproportionately affecting children [2] .As of 14 September 2023, Alberta Health Services reported 250 E. coli-related cases. Of these, 30 required hospitalization, and 20 patients, including those under intensive care, developed HUS, an acute complication from E. coli infections [1] . HUS can result in renal failure and, in extreme cases, fatality [3] . The rising HUS cases in Alberta's pediatric hospitals mirror the E. coli epidemic, emphasizing the need for urgent medical interventions.The epidemiological landscape of the E. coli outbreak in Alberta, as of 14 September 2023, presents a complex and concerning situation. Alberta Health Services has diligently documented a total of 250 cases with confirmed links to E. coli

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“…However, in 2018, O157:H7 was still the most common STEC serotype isolated from clinical cases (34.48%), followed by O26:H11 (11.49%), O111:NM (5.75%), and O103:H2 (5.75%) (PHAC, 2019). The highly pathogenic nature of O157:H7 makes it a significant food safety concern in North America (Noftall et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Lactic acid bacteria and spoilage bacteria: Their interactions in Escherichia coliO157:H7 biofilms on food contact surfaces and implications for beef contamination

Nan,

Rodas‐Gonzalez,

Stanford

et al. 2023

Journal of Food Safety

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This research explores the interaction between Shiga toxin‐producing Escherichia coli (STEC) O157:H7 and bacteria species commonly found in beef processing environments, specifically Carnobacterium, Lactobacillus, Comamonas, Raoultella, and Pseudomonas. The study investigated how various environmental conditions impact the formation of biofilms and the ability of O157:H7 to transfer from multispecies biofilm onto beef surfaces. For this purpose, a mixture of lactic acid bacteria (LAB), spoilage bacteria (106 CFU/mL), and E. coli O157 (103 CFU/mL) were combined as follows: LAB (T1): Carnobacterium piscicola + Lactobacillus bulgaricus + O157:H7, an spoilage bacteria (T2): Comamonas koreensis + Raoultella terrigena + O157:H7, an spoilage bacteria (T3): Pseudomonas aeruginosa + C. koreensis strain + O157:H7 and only O157:H7 as control (T4). Multispecies biofilms were developed on thermoplastic polyurethane (TPU) and stainless steel (SS) coupons at 10 and 25°C for 6 days, washed and stored for 6, 30, and 60 days at wet (60%–90% RH) and dry (20%–50%, RH) conditions. To evaluate O157:H7 transfer, beef cubes (3 × 3 × 1 cm) were placed on the coupons, followed by a 50‐g weight (7.35 kPa). The experiment was repeated three times in triplicate for each strain combination. Results demonstrate that biofilms formed at 10°C were generally weaker (less biomass) than those at 25°C. Regardless of temperature, more viable O157:H7 cells were transferred to beef from moist biofilms on TPU surfaces. At 25°C, T3 biofilm exhibited the lowest O157:H7 transfer to beef by 1.44 log10 CFU/cm2 (p < 0.01). At 10°C, none of the multispecies biofilm (T1–T3) affected the number of O157:H7 transfers to beef (p > 0.05). Notably, O157:H7 was not detected on food contact surfaces with 30 and 60‐day‐old dry biofilms (T1–T4). Through enrichment, E. coli O157:H7 was recovered from multispecies biofilms T1, T2, and T3. Findings from this study imply that multispecies biofilms contribute to the persistence of O157:H7 under dry conditions, regardless of temperature. These results underscore the intricate influence of multiple environmental factors—including surface type, biofilm age, humidity, temperature, and the presence of other bacterial species—on the risk of beef contamination facilitated by biofilms.

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Shiga toxin–producing Escherichia coli in British Columbia, 2011–2017: Analysis to inform exclusion guidelines

Cited by 10 publications

References 31 publications

Epidemiology of Non-O157 Shiga Toxin-Producing Escherichia coli in the Province of Alberta, Canada, from 2018 to 2021

Epidemiology of Non-O157 Shiga Toxin-Producing Escherichia coli in the Province of Alberta, Canada, from 2018 to 2021

Outbreak investigation and implications: assessing the severity of E. coli outbreak in Alberta, pediatric hospitalizations, and the rise of hemolytic uremic syndrome

Lactic acid bacteria and spoilage bacteria: Their interactions in Escherichia coliO157:H7 biofilms on food contact surfaces and implications for beef contamination

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