Shifts in the Fecal Microbial Community of Cystoisospora suis Infected Piglets in Response to Toltrazuril

Shrestha, Aruna; Metzler‐Zebeli, Barbara U.; Karembe, Hamadi; Šperling, Daniel; Koger, Simone; Joachim, Anja

doi:10.3389/fmicb.2020.00983

Cited by 12 publications

(10 citation statements)

References 72 publications

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“…Loss of gastrointestinal integrity and increased motility following C. muris infection may account for the greatly reduced abundance of some major anaerobic commensal bacteria such as Bacteroides S24-7. The would be consistent with results of Cystoisospora suis infection ( 31 ). Other bacterial genera such as Prevotella were also disturbed in our study.…”

Section: Discussionsupporting

confidence: 91%

Microbiome-Metabolomics Analysis of the Impacts of Cryptosporidium muris Infection in BALB/C Mice

et al. 2023

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Section: Discussionsupporting

confidence: 91%

Microbiome-Metabolomics Analysis of the Impacts of Cryptosporidium muris Infection in BALB/C Mice

et al. 2023

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“…Contrasting the latter, the presence of bacterial DNA in amniotic fluid [4] allows for assuming that gut microbial activity and compounds from microbial metabolism may play a role in fetal development in late gestation. Likewise, meconium contains relevant concentrations of SCFA (especially acetate and propionate), which supports that microbial action in a piglet's gut commences before Nutrients 2022, 14, 2524 2 of 15 birth [3,5,6]. Short-chain fatty acids (SCFA) may also reach the fetuses via the placenta, as small concentrations are present in maternal blood [7,8].…”

Section: Introductionmentioning

confidence: 73%

Short-Chain Fatty Acids Modulate Permeability, Motility and Gene Expression in the Porcine Fetal Jejunum Ex Vivo

et al. 2022

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Postnatally, short-chain fatty acids (SCFA) are important energetic and signaling agents, being involved in host nutrition, gut imprinting and immune and barrier function. Whether SCFA exert similar effects during the late fetal phase has been insufficiently elucidated. This study aimed to evaluate whether the fetal jejunum senses SCFA and whether SCFA modify the muscle tension and epithelial permeability and related signaling in jejunal tissue from the porcine fetus in late gestation. Exposure of fetal jejunal tissue to a mix of SCFA (70 µmol/mL) in an organ bath for 20 min lowered the muscle tension. Moreover, SCFA decreased the transepithelial conductance while increasing the short-circuit current in the Ussing chamber, indicating reduced permeability and increased SCFA absorption. Gene expression in the tissues harvested from the Ussing chamber after 30 min indicated downregulation of the expression of receptors (i.e., FFAR2 and TLR2), MCT1 and tight-junction and adherens proteins, which may be a negative feedback response to the applied high SCFA concentration compared with the micromolar concentration detected in fetal gastric fluid. Taken together, our data demonstrate that the fetal jejunum senses SCFA, which trigger electrophysiological, muscle contraction and related gene transcription responses. Hence, SCFA may play a role in prenatal gut nutrition and imprinting.

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“…Merogony and gamogony take place entirely in one host and the sporogony in the environment. After ingestion of sporulated oocysts, invasive stages are released and infect the gut epithelial cells to reproduce asexually within an intracellular vacuole 6 . The final generation of merozoites is considered to be sexually committed as the first step towards sexual development.…”

Section: Introductionmentioning

confidence: 99%

The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia)

Cruz‐Bustos

Feix

Lyrakis

et al. 2022

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The apicomplexan parasite Cystoisospora suis is an enteropathogen of suckling piglets with woldwide distribution. As with all coccidian parasites, its lifecycle is characterized by asexual multiplication followed by sexual development with two morphologically distinct cell types that presumably fuse to form a zygote from which the oocyst arises. However, knowledge of the sexual development of C. suis is still limited. To complement previous in vitro studies, we analysed transcriptional profiles at three different time points of development (corresponding to asexual, immature and mature sexual stages) in vitro via RNASeq. Overall, transcription of genes encoding proteins with important roles in gametes biology, oocyst wall biosynthesis, DNA replication and axonema formation as well as proteins with important roles in merozoite biology was identified. A homologue of an oocyst wall tyrosine rich protein of Toxoplasma gondii was expressed in macrogametes and oocysts of C. suis. We evaluated inhibition of sexual development in a host-free culture for C. suis by antiserum specific to this protein to evaluate whether it could be exploited as a candidate for control strategies against C. suis. Based on these data, targets can be defined for future strategies to interrupt parasite transmission during sexual development.

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Shifts in the Fecal Microbial Community of Cystoisospora suis Infected Piglets in Response to Toltrazuril

Cited by 12 publications

References 72 publications

Microbiome-Metabolomics Analysis of the Impacts of Cryptosporidium muris Infection in BALB/C Mice

Microbiome-Metabolomics Analysis of the Impacts of Cryptosporidium muris Infection in BALB/C Mice

Short-Chain Fatty Acids Modulate Permeability, Motility and Gene Expression in the Porcine Fetal Jejunum Ex Vivo

The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia)

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