Shifts in soil microbial community, soil enzymes and crop yield under peanut/maize intercropping with reduced nitrogen levels

Chen, Jun; Arafat, Yasir; Wu, Linkun; Xiao, Zhigang; Li, Qisong; Khan, Muhammad Azam; Khan, Muhammad Umar; Lin, Shan; Lin, Wenxiong

doi:10.1016/j.apsoil.2017.11.010

Cited by 63 publications

(38 citation statements)

References 51 publications

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“…The total and available essential nutrients (N, P, and K) were measured, according to Reference [25]. Soil urease [EC 3.5.1.5] activity was determined by incubating 5 g soil with 30 mL of extracting solution at 37 • C for 24 h. The formation of ammonium was measured spectrophotometrically at 578 nm [26].…”

Section: Analysis Of Essential Nutrients (Npk) and Soil Enzymatic Actmentioning

confidence: 99%

Restoration of Long-Term Monoculture Degraded Tea Orchard by Green and Goat Manures Applications System

et al. 2019

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Tea is an economic shrubby plant in tropical and subtropical regions of the world. To obtain high yield in tea cultivation, chemical fertilizer application rates have generally been used. However, a large quantity of chemical fertilizer application in a long-term continuously ratooned and monoculture tea orchard can inevitably lead to soil acidification and a decline in fertility. Therefore, the restoration of soil fertility and the sustainable development of tea planting by organic ways are critical for the tea industry. In this study, field trials were conducted in the tea orchard that was continuously ratooned and mono-cultured for 20 years. Nitrogen fertilizer (NF), Laredo soybeans green manure (LF), and goat manure (GM) treatments were applied to restore optimum acidity, soil fertility, microbial activity, and the community structure of a long-term continuously monoculture tea orchard. This paper investigated that the pH value was increased from 4.23 to 4.32 in GM and LF, respectively. Similarly, the content of exchangeable acidity (EA) was decreased by 1.21 and 1.46 cmol·kg−1 in GM and LF, respectively. Available nutrient results indicated that the content of NH4+-N was increased by 3.96, 4.38, NO3−-N by 1.07, 2.16, AP by 3.46, 6.86, AK by 0.26, 0.3 mg kg−1 in GM and LF treatments, respectively. Enzyme analysis revealed that the activity of urease and sucrase was promoted by 7.98 mg·g−1·24 h−1 and 6.77 mg·g−1·24 h−1, respectively, in LF treatment. Likewise, the activity of acid phosphatase and polyphenol oxidase was sharply increased by 2.3 mg·g−1 h−1 and 63.07 mg·g−1 h−1 in LF treatments. Additionally, the activity of urease, sucrase, acidic phosphatase, polyphenol oxidase, and peroxidase were also significantly increased by applying GM treatments. Meanwhile, LF and GM treatments significantly improved soil microbial biomass as well as low weight organic acid content in degraded tea rhizosphere. Furthermore, high throughput sequence results illustrated that the relative abundance of Rhizobiaceae and Bradyrhizobiaceae families increased in LF and GM treatments, respectively, which are mostly a kind of nitrogen fixer and plant growth promoting bacteria. Taken together, the physiological traits of the new sprouts and the biochemical components of new tea leaves were also significantly improved by GM and LF treatments. From this study, it is concluded that LF and GM are good agriculture management practices, which promote plant growth, yield, and nutrient availability by maintaining and improving pH, enhancing available nutrients status, improving the secretion of low molecular weight organic acids, and balancing the microbial community structure in the long-term mono-cultured tea orchard.

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Section: Analysis Of Essential Nutrients (Npk) and Soil Enzymatic Actmentioning

confidence: 99%

Restoration of Long-Term Monoculture Degraded Tea Orchard by Green and Goat Manures Applications System

et al. 2019

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“…Li et al (2016) proposed that below-surface interactions in an maize/peanut intercropping system considerably altered the microbial structure of the soil and the dominant microbial species that are strongly linked to soil N availability. Chen et al (2018) revealed that peanut/maize intercropping promotes plant N uptake and increases the abundance of N-cycling organisms and other beneficial rhizosphere bacteria. Lian et al (2019) demonstrated that sugarcane/soybean intercropping in acidic soil increases microbial diversity and shifts soil microbial communities, which may stimulate N accumulation by the legume.…”

Section: Introductionmentioning

confidence: 99%

Intercropping Alters the Soil Microbial Diversity and Community to Facilitate Nitrogen Assimilation: A Potential Mechanism for Increasing Proso Millet Grain Yield

et al. 2020

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Intercropping of cereals and legumes has been used in modern agricultural systems, and the soil microorganisms associated with legumes play a vital role in organic matter decomposition and nitrogen (N) fixation. This study investigated the effect of intercropping on the rhizosphere soil microbial composition and structure and how this interaction affects N absorption and utilization by plants to improve crop productivity. Experiments were conducted to analyze the rhizosphere soil microbial diversity and the relationship between microbial composition and N assimilation by proso millet (Panicum miliaceum L.) and mung bean (Vigna radiata L.) from 2017 to 2019. Four different intercropping row arrangements were evaluated, and individual plantings of proso millet and mung bean were used as controls. Microbial diversity and community composition were determined through Illumina sequencing of 16S rRNA and internal transcribed spacer (ITS) genes. The results indicated that intercropping increased N levels in the soil–plant system and this alteration was strongly dependent on changes in the microbial (bacterial and fungal) diversities and communities. The increase in bacterial alpha diversity and changes in unique operational taxonomic unit (OTU) numbers increased the soil N availability and plant N accumulation. Certain bacterial taxa (such as Proteobacteria) and fungal taxa (such as Ascomycota) were significantly altered under intercropping and showed positive responses to increased N assimilation. The average grain yield of intercropped proso millet increased by 13.9–50.1% compared to that of monoculture proso millet. Our data clearly showed that intercropping proso millet with mung bean altered the rhizosphere soil microbial diversity and community composition; thus, this intercropping system represents a potential mechanism for promoting N assimilation and increasing grain yield.

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“…Shifts in the structure and composition of the microbial community are strong indicators of soil biological activity, soil quality and crop productivity of terrestrial agro-ecosystems (Edmeades, 2003). Based on results of diversity, the NF led to a significant increase ( P ≤ 0.05) in the Shannon, Pielou, and Simpson diversity indices compared with the NT soil (Table 5), which led to more stable soil environment for plants (Chen et al, 2018). As we know, soil contains the largest pool of carbon on earth (Batjes, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…The diverse soil nutrients and plant secondary metabolites present in the exudates enhance the enrichment of specific functional or taxonomic bacterial groups residing in the rhizosphere (Bais et al, 2006; Zahar et al, 2008). Currently, various DNA-dependent strategies, such as denaturing gradient gel electrophoresis (DGGE) (Muyzer and Smalla, 1998; Chen et al, 2017), terminal restriction fragment length polymorphism (T-RFLP) (Osborn et al, 2000; Chen et al, 2018), and 16S rRNA gene sequence have been utilized to determine the biological diversity and composition of microbial communities in soil ecosystems (Kim et al, 2012; Arafat et al, 2017). However, comprehensive characterization of biological processes needs integrated approaches (Griffin et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Nitrogen Fertilizer Amendment Alter the Bacterial Community Structure in the Rhizosphere of Rice (Oryza sativa L.) and Improve Crop Yield

et al. 2019

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Availability of nitrogen (N) in soil changes the composition and activities of microbial community, which is critical for the processing of soil organic matter and health of crop plants. Inappropriate application of N fertilizer can alter the rhizosphere microbial community and disturb the soil N homeostasis. The goal of this study was to assess the effect of different ratio of N fertilizer at various early to late growth stages of rice, while keeping the total N supply constant on rice growth performance, microbial community structure, and soil protein expression in rice rhizosphere. Two different N regimes were applied, i.e., traditional N application (NT) consists of three sessions including 60, 30 and 10% at pre-transplanting, tillering and panicle initiation stages, respectively, while efficient N application (NF) comprises of four sessions, i.e., 30, 30, 30, and 10%), where the fourth session was extended to anthesis stage. Soil metaproteomics combined with Terminal Restriction Fragment Length Polymorphism (T-RFLP) were used to determine the rhizosphere biological process. Under NF application, soil enzymes, nitrogen utilization efficiency and rice yield were significantly higher compared to NT application. T-RFLP and qPCR analysis revealed differences in rice rhizosphere bacterial diversity and structure. NF significantly decreased the specific microbes related to denitrification, but opposite result was observed for bacteria associated with nitrification. Furthermore, soil metaproteomics analysis showed that 88.28% of the soil proteins were derived from microbes, 5.74% from plants, and 6.25% from fauna. Specifically, most of the identified microbial proteins were involved in carbohydrate, amino acid and protein metabolisms. Our experiments revealed that NF positively regulates the functioning of the rhizosphere ecosystem and further enabled us to put new insight into microbial communities and soil protein expression in rice rhizosphere.

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Shifts in soil microbial community, soil enzymes and crop yield under peanut/maize intercropping with reduced nitrogen levels

Cited by 63 publications

References 51 publications

Restoration of Long-Term Monoculture Degraded Tea Orchard by Green and Goat Manures Applications System

Restoration of Long-Term Monoculture Degraded Tea Orchard by Green and Goat Manures Applications System

Intercropping Alters the Soil Microbial Diversity and Community to Facilitate Nitrogen Assimilation: A Potential Mechanism for Increasing Proso Millet Grain Yield

Nitrogen Fertilizer Amendment Alter the Bacterial Community Structure in the Rhizosphere of Rice (Oryza sativa L.) and Improve Crop Yield

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