Shifts in bacterial community composition and abundance of nitrifiers during aerobic granulation in two nitrifying sequencing batch reactors

Fan, Xiao-Yan; Gao, Jingfeng; Pan, Kai-Ling; Li, Dingchang; Zhang, Lifang; Wang, Shijie

doi:10.1016/j.biortech.2017.12.038

Cited by 46 publications

(25 citation statements)

References 33 publications

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“…Both microbial diversity and richness decreased during granulation with the genera Rhodanobacter dominating at the maturation stage of granulation which coincided with the increase in protein content of the EPS. Fan et al ( 2018 ) compared granulation in identical reactors operated either with domestic wastewater or synthetic wastewater with the same COD and N load. Illumina sequencing showed a sharp decrease in bacterial diversity during the granulation with a different bacterial community structure in the granules compared to the seed sludge.…”

Section: Sludge Granulationmentioning

confidence: 99%

The mechanisms of granulation of activated sludge in wastewater treatment, its optimization, and impact on effluent quality

Wilén

Liébana

Persson

et al. 2018

Appl Microbiol Biotechnol

155

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Granular activated sludge has gained increasing interest due to its potential in treating wastewater in a compact and efficient way. It is well-established that activated sludge can form granules under certain environmental conditions such as batch-wise operation with feast-famine feeding, high hydrodynamic shear forces, and short settling time which select for dense microbial aggregates. Aerobic granules with stable structure and functionality have been obtained with a range of different wastewaters seeded with different sources of sludge at different operational conditions, but the microbial communities developed differed substantially. In spite of this, granule instability occurs. In this review, the available literature on the mechanisms involved in granulation and how it affects the effluent quality is assessed with special attention given to the microbial interactions involved. To be able to optimize the process further, more knowledge is needed regarding the influence of microbial communities and their metabolism on granule stability and functionality. Studies performed at conditions similar to full-scale such as fluctuation in organic loading rate, hydrodynamic conditions, temperature, incoming particles, and feed water microorganisms need further investigations.

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Section: Sludge Granulationmentioning

confidence: 99%

The mechanisms of granulation of activated sludge in wastewater treatment, its optimization, and impact on effluent quality

Wilén

Liébana

Persson

et al. 2018

Appl Microbiol Biotechnol

155

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“…In addition, the high abundance of amoA transcripts from comammox Nitrospira detected in activated sludge suggests an active role in nitrification [23,24]. Previous studies examining comammox Nitrospira in WWTPs focused primarily on activated sludge secondary treatment systems and sequencing batch reactors [12,[15][16][17][18][19][20][21][22][23][25][26][27][28]. To our knowledge, no studies have investigated comammox Nitrospira in biofilm-based tertiary wastewater treatment systems.…”

Section: Introductionmentioning

confidence: 99%

High functional diversity among Nitrospira populations that dominate rotating biological contactor microbial communities in a municipal wastewater treatment plant

et al. 2020

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Nitrification, the oxidation of ammonia to nitrate via nitrite, is an important process in municipal wastewater treatment plants (WWTPs). Members of the Nitrospira genus that contribute to complete ammonia oxidation (comammox) have only recently been discovered and their relevance to engineered water treatment systems is poorly understood. This study investigated distributions of Nitrospira, ammonia-oxidizing archaea (AOA), and ammonia-oxidizing bacteria (AOB) in biofilm samples collected from tertiary rotating biological contactors (RBCs) of a municipal WWTP in Guelph, Ontario, Canada. Using quantitative PCR (qPCR), 16S rRNA gene sequencing, and metagenomics, our results demonstrate that Nitrospira species strongly dominate RBC biofilm samples and that comammox Nitrospira outnumber all other nitrifiers. Genome bins recovered from assembled metagenomes reveal multiple populations of comammox Nitrospira with distinct spatial and temporal distributions, including several taxa that are distinct from previously characterized Nitrospira members. Diverse functional profiles imply a high level of niche heterogeneity among comammox Nitrospira, in contrast to the sole detected AOA representative that was previously cultivated and characterized from the same RBC biofilm. Our metagenome bins also reveal two cyanase-encoding populations of comammox Nitrospira, suggesting an ability to degrade cyanate, which has only been shown previously for several Nitrospira representatives that are strict nitrite oxidizers. This study demonstrates the importance of RBCs as model systems for continued investigation of environmental factors that control the distributions and activities of AOB, AOA, comammox Nitrospira, and other nitrite oxidizers.

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“…Additionally, the high abundance of amoA transcripts from comammox Nitrospira detected in activated sludge suggests 65 that these bacteria actively contribute to nitrification [23]. Previous studies examining comammox Nitrospira in WWTPs focused primarily on activated sludge secondary treatment systems and sequencing batch reactors [12,[15][16][17][18][19][20][21][22][23][24][25][26][27]. To date, no studies have investigated comammox Nitrospira in biofilm-based tertiary wastewater treatment systems.Rotating biological contactors (RBCs; Figure 1A) represent a full-scale tertiary system 70 treating municipal waste from ~132,000 residents of Guelph, Ontario, Canada.…”

mentioning

confidence: 99%

High functional diversity among Nitrospira populations that dominate rotating biological contactor microbial communities in a municipal wastewater treatment plant

Spasov

Tsuji

Hug

et al. 2019

Preprint

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AbstractNitrification, the oxidation of ammonia to nitrate via nitrite, is an important process in municipal wastewater treatment plants (WWTPs). Members of the Nitrospira genus that contribute to complete ammonia oxidation (comammox) have only recently been discovered and their relevance to engineered water treatment systems is poorly understood. This study investigated distributions of Nitrospira, ammonia-oxidizing archaea (AOA), and ammonia-oxidizing bacteria (AOB) in biofilm samples collected from tertiary rotating biological contactors (RBCs) of a municipal WWTP in Guelph, Ontario, Canada. Using quantitative PCR (qPCR), 16S rRNA gene sequencing, and metagenomics, our results demonstrate that Nitrospira species strongly dominate RBC biofilm samples and that comammox Nitrospira outnumber all other nitrifiers. Genome bins recovered from assembled metagenomes reveal multiple populations of comammox Nitrospira with distinct spatial and temporal distributions, including several taxa that are distinct from previously characterized Nitrospira members. Diverse functional profiles imply a high level of niche heterogeneity among comammox Nitrospira, in contrast to the sole detected AOA representative that was previously cultivated and characterized from the same RBC biofilm. Our metagenome bins also reveal two cyanase-encoding populations of comammox Nitrospira, suggesting an ability to degrade cyanate, which has not been shown previously for Nitrospira that are not strict nitrite oxidizers. This study demonstrates the importance of RBCs as model systems for continued investigation of environmental factors that control the distributions and activities of AOB, AOA, comammox Nitrospira, and other nitrite oxidizers.

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Shifts in bacterial community composition and abundance of nitrifiers during aerobic granulation in two nitrifying sequencing batch reactors

Cited by 46 publications

References 33 publications

The mechanisms of granulation of activated sludge in wastewater treatment, its optimization, and impact on effluent quality

The mechanisms of granulation of activated sludge in wastewater treatment, its optimization, and impact on effluent quality

High functional diversity among Nitrospira populations that dominate rotating biological contactor microbial communities in a municipal wastewater treatment plant

High functional diversity among Nitrospira populations that dominate rotating biological contactor microbial communities in a municipal wastewater treatment plant

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