Shifting the distribution: modulation of the lipid profile in Yarrowia lipolytica via iron content

Cordova, Lauren T.; Palmer, Claire M.; Alper, Hal S.

doi:10.1007/s00253-022-11800-w

Cited by 10 publications

(3 citation statements)

References 37 publications

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“…Moreover, the cells showed a change in the lipid distribution, particularly a decrease in stearic acid (C18:0) content and an increase in linoleic acid (C18:2). This shift in production is a great opportunity to explore unsaturated fatty acid production, and as an example, it was shown that iron supplementation coupled with a complex media can increase α-linolenic acid production in previously developed strains by 2.6-fold (Cordova et al., 2022 ).…”

Section: Trace Metals and Mineralsmentioning

confidence: 99%

Strategic nutrient sourcing for biomanufacturing intensification

Noroozi,

Jarboe

2023

Journal of Industrial Microbiology and Biotechnology

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The successful design of economically viable bioprocesses can help to abate global dependence on petroleum, increase supply chain resilience, and add value to agriculture. Specifically, bioprocessing provides the opportunity to replace petrochemical production methods with biological methods and to develop novel bioproducts. Even though a vast range of chemicals can be biomanufactured, the constraints of economic viability, especially while competing with petrochemicals, are severe. There have been extensive gains in our ability to engineer microbes for improved production metrics and for utilization of target carbon sources. The impact of growth medium composition on process cost and organism performance receives less attention in the literature than organism engineering efforts, with media optimization often being performed in proprietary settings. The widespread use of corn steep liquor (CSL) as a nutrient source demonstrates the viability and importance of ‘waste’ streams in biomanufacturing. There are other promising waste streams that can be used to increase the sustainability of biomanufacturing, such as the use of urea instead of fossil fuel-intensive ammonia, and the use of struvite instead of contributing to the depletion of phosphate reserves. In this review, we discuss several process-specific optimizations of micronutrients that increased product titers by 2-fold or more. This practice of deliberate and thoughtful sourcing and adjustment of nutrients can substantially impact process metrics. Yet the mechanisms are rarely explored, making it difficult to generalize results to other processes. In this review, we will discuss examples of nutrient sourcing and adjustment as a means of process improvement.

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Section: Trace Metals and Mineralsmentioning

confidence: 99%

Strategic nutrient sourcing for biomanufacturing intensification

Noroozi,

Jarboe

2023

Journal of Industrial Microbiology and Biotechnology

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“…In detail, a 2.5 L minimal medium batch reactor, 1 g/L C18:1 and 0.22 g/L C16:1 were produced after 24 h at 27°C [161]. The lipid production within the cells can be increased twofold by supplementing additional iron [162]. For Y. lipolytica the highest reported fatty acid production was achieved in a batch reactor with YPD medium at 30°C and a pH of 5.5 after 120 h, resulting in 56.14 g/L of which 84 % compromised C18:1 [163].…”

Section: Comparison Of E Coli and Other Organisms For The Production ...mentioning

confidence: 99%

Strategies to Enhance the Biosynthesis of Monounsaturated Fatty Acids in Escherichia coli

Matthay

Schalck

Verstraeten

et al. 2023

Biotechnol Bioproc E

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Fueled by a variety of industrial applications ranging from bioplastics to cosmetics and pharmaceuticals, the global demand for unsaturated fatty acids is steadily rising. Most of these applications build on monounsaturated fatty acids with a chain length of 16 or 18 carbon atoms, rendering these compounds valuable industrial assets. While of high industrial interest, monounsaturated fatty acids with a chain length of 8 to 12 carbon atoms are hard to produce using conventional chemical or plant-based production ways. As a consequence, these compounds are expensive and not readily available for large-scale industrial applications. Recent advances in metabolic engineering have put forward microbes as cost-efficient factories to produce numerous chemical compounds. In this respect, the model organism Escherichia coli is considered an interesting species as it can grow on various feedstocks and a plethora of genetic information is available, facilitating expression of exogenous enzymes. For the purpose of shifting the fatty acid pool towards monounsaturated fatty acid, thioesterases and desaturases represent suitable candidate enzymes. The former stop chain elongation, reacting on acyl-chains of specific chain length and saturation level, whereas the latter directly target fatty acids to convert them into unsaturated analogues. In this review we summarize thioesterases and desaturases that have been introduced in E. coli to enrich unsaturated fatty acids. Furthermore, we discuss advantages of using bacteria for the production of designer compounds including but not limited to medium-chain monounsaturated fatty acids.

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“…Furthermore, the engineered strain L36DGA1 produced 1.4 g/L ALA in a bioreactor with an operating volume of 2 L under the same culture conditions [ 47 ]. Additionally, Cordova et al [ 77 ] also successfully explored the positive effect of trace metals, especially iron (Ⅱ), on cell growth and production of PUFAs, such as ALA. They firstly examined strains Po1f and Po1f Δ pex10 Δ mfe1 DGA1 (abbreviated as Po1fpmD), and found that the addition of iron (Ⅱ) not only strongly increased the LA content at the expense of stearic acid, but also significantly increased the final optical density and lipid accumulation.…”

Section: Engineering Yarrowia Lipolytica To Produc...mentioning

confidence: 99%