Shexiang Baoxin Pill attenuates myocardial ischemia/reperfusion injury by activating autophagy via modulating the ceRNA-Map3k8 pathway

Yu, Yong-Wei; Liu, Shuai; Zhou, Yingying; Huang, Kaiyu; Wu, Bo-Sen; Lin, Zhi-hui; Zhu, Chen-xi; Xue, Yan; Ji, Kangting

doi:10.1016/j.phymed.2022.154336

Cited by 18 publications

(9 citation statements)

References 33 publications

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“…Because different cell death types are not completely isolated and may have a synergistic effect on the progression of I/R injury [ 117 ], future studies exploring the interactions between different types of cell death and identifying medicines that could simultaneously regulate multiple targets are urgently required. In this context, for instance, Yu et al reported that the Shexiang Baoxin Pill (SBP) could mitigate myocardial I/R injury by interfering with autophagy and inhibiting myocardial pyroptosis at the same time [ 118 ]. Given the fact that caspase-3 plays a pivotal role both in apoptosis and GSDME-mediated pyroptosis, it is reasonable to deduce that there must be some interaction between pyroptosis and apoptosis during I/R injury.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Pyroptosis: A Newly Discovered Therapeutic Target for Ischemia-Reperfusion Injury

Zheng

Chi

et al. 2022

Biomolecules

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Ischemia-reperfusion (I/R) injury, uncommon among patients suffering from myocardial infarction, stroke, or acute kidney injury, can result in cell death and organ dysfunction. Previous studies have shown that different types of cell death, including apoptosis, necrosis, and autophagy, can occur during I/R injury. Pyroptosis, which is characterized by cell membrane pore formation, pro-inflammatory cytokine release, and cell burst, and which differentiates itself from apoptosis and necroptosis, has been found to be closely related to I/R injury. Therefore, targeting the signaling pathways and key regulators of pyroptosis may be favorable for the treatment of I/R injury, which is far from adequate at present. This review summarizes the current status of pyroptosis and its connection to I/R in different organs, as well as potential treatment strategies targeting it to combat I/R injury.

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Section: Conclusion and Perspectivementioning

confidence: 99%

Pyroptosis: A Newly Discovered Therapeutic Target for Ischemia-Reperfusion Injury

Zheng

Chi

et al. 2022

Biomolecules

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“…Therefore, the equivalent dose for a mouse was 27.75 mg/kg bw/d. This dose has exhibited cardioprotective efficacy without toxicity in previous studies ( Yu et al, 2022 ). Furthermore, considering that the maximum daily volume of gavage in a mouse is approximately 0.01 mL/g bw, we set the SBP concentration to 2.775 mg/mL.…”

Section: Methodsmentioning

confidence: 78%

Single-nucleus ribonucleic acid-sequencing and spatial transcriptomics reveal the cardioprotection of Shexiang Baoxin Pill (SBP) in mice with myocardial ischemia-reperfusion injury

et al. 2023

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Graphical AbstractSingle-cell landscape of SBP treatments in a mouse model of myocardial I/R injury. A total of 75,546 cells from nine mouse cardiac tissues in 3 different groups are projected by UMAP plot. The four colored tracks (from inside to outside) indicate cluster, origin ID, group and cell type. The axis outside the circular plot depicts the scale of the total cell number for each class. The text in each of the four corners show an overview of the role of SBP on cardiomyocytes, endothelial cells, fibroblasts, and macrophages respectively. Legends denote the origin ID, group, cell type and cluster.

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“…ROS and DHE staining can reflect oxidative stress level. 16 The 6-well plates were used to culture H9c2 cells. After each group was treated, the supernatant was removed, washed thrice with PBS in the dark, and ROS (#S0033S, Beyotime) or DHE staining (#S0063, Beyotime) was performed for 30 min as directed by the manufacturer.…”

Section: Ros or Dhe Staining Assaymentioning

confidence: 99%

Dihydroartemisinin alleviates doxorubicin‐induced cardiotoxicity and ferroptosis by activating Nrf2 and regulating autophagy

Lin,

Xiang,

et al. 2024

The FASEB Journal

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Although the use of Doxorubicin (Dox) is extensive in the treatment of malignant tumor, the toxic effects of Dox on the heart can cause myocardial injury. Therefore, it is necessary to find an alternative drug to alleviate the Dox‐induced cardiotoxicity. Dihydroartemisinin (DHA) is a semisynthetic derivative of artemisinin, which is an active ingredient of Artemisia annua. The study investigates the effects of DHA on doxorubicin‐induced cardiotoxicity and ferroptosis, which are related to the activation of Nrf2 and the regulation of autophagy. Different concentrations of DHA were administered by gavage for 4 weeks in mice. H9c2 cells were pretreated with different concentrations of DHA for 24 h in vitro. The mechanism of DHA treatment was explored through echocardiography, biochemical analysis, real‐time quantitative PCR, western blotting analysis, ROS/DHE staining, immunohistochemistry, and immunofluorescence. In vivo, DHA markedly relieved Dox‐induced cardiac dysfunction, attenuated oxidative stress, alleviated cardiomyocyte ferroptosis, activated Nrf2, promoted autophagy, and improved the function of lysosomes. In vitro, DHA attenuated oxidative stress and cardiomyocyte ferroptosis, activated Nrf2, promoted clearance of autophagosomes, and reduced lysosomal destruction. The changes of ferroptosis and Nrf2 depend on selective degradation of keap1 and recovery of lysosome. We found for the first time that DHA could protect the heart from the toxic effects of Dox‐induced cardiotoxicity. In addition, DHA significantly alleviates Dox‐induced ferroptosis through the clearance of autophagosomes, including the selective degradation of keap1 and the recovery of lysosomes.

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Shexiang Baoxin Pill attenuates myocardial ischemia/reperfusion injury by activating autophagy via modulating the ceRNA-Map3k8 pathway

Cited by 18 publications

References 33 publications

Pyroptosis: A Newly Discovered Therapeutic Target for Ischemia-Reperfusion Injury

Pyroptosis: A Newly Discovered Therapeutic Target for Ischemia-Reperfusion Injury

Single-nucleus ribonucleic acid-sequencing and spatial transcriptomics reveal the cardioprotection of Shexiang Baoxin Pill (SBP) in mice with myocardial ischemia-reperfusion injury

Dihydroartemisinin alleviates doxorubicin‐induced cardiotoxicity and ferroptosis by activating Nrf2 and regulating autophagy

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