Shewanella xiamenensis sp. nov., isolated from coastal sea sediment

Abstract

A Gram-negative, motile, rod-shaped bacterium, strain S4 T , was isolated from coastal sediment collected off Xiamen, China. The physiological and biochemical features of strain S4 T , determined using the API 20NE, API ZYM and Biolog GN2 systems, were similar to those of members of the genus Shewanella. Phylogenetic analyses based on 16S rRNA and gyrB gene sequences placed strain S4 T in the genus Shewanella, and it was most closely related to Shewanella oneidensis and related species. DNA-DNA hybridization demonstrated only 11.9-30.4 % relatedness between S4 T and the type strains of related Shewanella species. On the basis of phylogenetic and phenotypic characteristics, strain S4 T is classified in the genus Shewanella as a representative of a distinct novel species, for which the name Shewanella xiamenensis sp. nov. is proposed. The type strain is S4 T (5CCTCC M 209017 T 5JCM 16212 T ).

Nosocomial peripancreatic infection associated with Shewanella xiamenensis

“…Biochemical characteristics (Table 2) of WCJ25 were determined using the API 20E and 20NE systems (bioMérieux). Strain WCJ25 exhibited a biochemical pattern similar to that of S. xiamenensis type strain S4 (Huang et al, 2010), except that WCJ25 did not utilize glucose, arabinose and caprate (Table 2). WCJ25 was susceptible to amikacin, gentamicin, tobramycin, cefotetan, imipenem, trimethoprim/sulphamethoxazole, levofloxacin, showed intermediate resistance to piperacillin/tazobactam and ciprofloxacin, and resistant to azithromycin, ampicillin, ampicillin-sulbactam, ceftazidime, cefepime, ceftriaxone and ceftizoxime.…”

“…Shewanella xiamenensis is a newly established species of the genus Shewanella; the type strain of this species was found in costal sea sediments off Xiamen, Fujian province, China (Huang et al, 2010). Although Shewanella species, especially Shewanella algae and Shewanella putrefaciens, have been recognized as human pathogens, there appear to have been no reports of the isolation of S. xiamenensis from any clinical samples and this species has not previously been documented as a cause of infection.…”

“…The 1382 bp 16S rRNA gene sequence of strain WCJ25 was closest (99.6 % identity) to Shewanella xiamenensis type strain S4 and was 98.1 % identical to Shewanella putrefaciens type strain ATCC 8071 (Table 1). As the 16S rRNA gene sequence sometimes lacks sufficient resolution to differentiate closely related Shewanella species (Huang et al, 2010), the more rapidly evolving gyrB gene was partially sequenced. The 1167 bp gyrB sequence of strain WCJ25 was 98.5 % identical to that of S. xiamenensis S4 and only 88.1 % identical to that of S. putrefaciens ATCC 8071 (Table 1).…”

Biofabrication of gold nanoparticles by Shewanella species

“…However, the MR-1 wild type and its mutants omcA and mtrC were still capable of reducing Au 3+ into Au@NPs (Wu et al 2013b), while the particle size of the extracellular nanoparticles were decreased mtrC and omcA mutants (Ng et al 2013). On the other hand, Shewanella xiamenensis, which is isolated from coastal sediment collected off Xiamen, China, is a close-related strain to S. oneidensis based on phylogenetic tree analysis by 16S rRNA and gyrase gene (Huang et al 2010). It has also been reported to reduce mediators from the medium to nanoparticles (Ng et al 2015a) and also showed resistance to different kinds of metal ions (Ng et al 2015b).…”

Adenosine monophosphate degradation and inosinic acid accumulation in the shrimp Penaeus japonicus

“…Exiguobacterium oxidotolerans has been isolated from a drain in a fish processing plant in Hokkaido (Yumoto et al 2004) and from sea mud (Chen et al 2010). Shewanella xiamenensis has been isolated from sea floor sediment (Ng et al 2014;Huang et al 2010). These three species of bacteria are present on the sea floor where shrimp live, so it was not surprising to encounter them in our samples.…”