Shewanella putrefaciens CN32 outer membrane cytochromes MtrC and UndA reduce electron shuttles to produce electricity in microbial fuel cells

Wu, Xian; Zou, Long; Huang, Yunhong; Qiao, Yan; Long, Zhong-er; Liu, Haoping; Li, Chang Ming

doi:10.1016/j.enzmictec.2018.04.005

Cited by 23 publications

(17 citation statements)

References 32 publications

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“…Considering the previously reported broad potential window of Shewanella membrane-bound cytochromes (Firer-Sherwood et al, 2008; Baron et al, 2009; Carmona-Martinez et al, 2011; Okamoto et al, 2011), it was suggested that the polyriboflavin interface not only can improve the MET but might well also promote the DET by means of a potential co-action of riboflavin and cytochromes. Strikingly, the PRF@CC-30 electrode delivered undetectable current response within the first 50 h when the ΔmtrC/undA mutant strain was used as an inoculum (Figure 3A) due to the crucial role of outer-membrane cytochromes in both DET and MET of Shewanella strains (Coursolle et al, 2010; Okamoto et al, 2011; Wu et al, 2018). The latter partial recovery might be owing to the increased cell membrane permeability for transporting mediators and/or the activation of other cytochromes for reducing mediators along with the progress of electrochemical acclimation (Wu et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

“…Strikingly, the PRF@CC-30 electrode delivered undetectable current response within the first 50 h when the ΔmtrC/undA mutant strain was used as an inoculum (Figure 3A) due to the crucial role of outer-membrane cytochromes in both DET and MET of Shewanella strains (Coursolle et al, 2010; Okamoto et al, 2011; Wu et al, 2018). The latter partial recovery might be owing to the increased cell membrane permeability for transporting mediators and/or the activation of other cytochromes for reducing mediators along with the progress of electrochemical acclimation (Wu et al, 2018). The Nyquist plots (Figure 3D) were conducted to analyze the interfacial charge-transfer resistance ( R ct ), which clearly demonstrated a much smaller R ct of the PRF@CC-30 electrode.…”

Section: Resultsmentioning

confidence: 99%

“…Strain S. putrefaciens CN32 was purchased from ATCC (Number: BAA-1097), and its mutant strain with in-frame deletion of genes encoding out-membrane cytochromes MtrC and UndA (designated as “ ΔmtrC/undA ” strain) was constructed in our previous work (Wu et al, 2018). All strains were routinely cultivated in Luria-Bertani (LB) broth (10 g/L tryptone, 5 g/L yeast extract (Oxoid, United Kingdom), 5 g/L NaCl, pH 7.2) at 30°C with shaking at 180 rpm to a desired optical density at 600 nm (OD 600 ) of 1.5.…”

Section: Methodsmentioning

confidence: 99%

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Promoting Shewanella Bidirectional Extracellular Electron Transfer for Bioelectrocatalysis by Electropolymerized Riboflavin Interface on Carbon Electrode

Zou

Huang

et al. 2019

Front. Microbiol.

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The extracellular electron transfer (EET) that connects the intracellular metabolism of electroactive microorganisms to external electron donors/acceptors, is the foundation to develop diverse microbial electrochemical technologies. For a particular microbial electrochemical device, the surface chemical property of an employed electrode material plays a crucial role in the EET process owing to the direct and intimate biotic-abiotic interaction. The functional modification of an electrode surface with redox mediators has been proposed as an effectual approach to promote EET, but the underlying mechanism remains unclear. In this work, we investigated the enhancement of electrochemically polymerized riboflavin interface on the bidirectional EET of Shewanella putrefaciens CN32 for boosting bioelectrocatalytic ability. An optimal polyriboflavin functionalized carbon cloth electrode achieved about 4.3-fold output power density (∼707 mW/m2) in microbial fuel cells and 3.7-fold cathodic current density (∼0.78 A/m2) for fumarate reduction in three-electrode cells compared to the control, showing great increases in both outward and inward EET rates. Likewise, the improvement was observed for polyriboflavin-functionalized graphene electrodes. Through comparison between wild-type strain and outer-membrane cytochrome (MtrC/UndA) mutant, the significant improvements were suggested to be attributed to the fast interfacial electron exchange between the polyriboflavin interface with flexible electrochemical activity and good biocompatibility and the outer-membrane cytochromes of the Shewanella strain. This work not only provides an effective approach to boost microbial electrocatalysis for energy conversion, but also offers a new demonstration of broadening the applications of riboflavin-functionalized interface since the widespread contribution of riboflavin in various microbial EET pathways together with the facile electropolymerization approach.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Promoting Shewanella Bidirectional Extracellular Electron Transfer for Bioelectrocatalysis by Electropolymerized Riboflavin Interface on Carbon Electrode

Zou

Huang

et al. 2019

Front. Microbiol.

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“…12,[27][28][29] Microbial activity affects the electrical performance of MFCs mainly through electron transfer. 13,14,30 In MFCs, electron transfer is mainly through two modes: (a) the direct electron transfer (DET) mode, (b) the mediated electron transfer (MET) mode. 15 In this paper, Figures 2a and 3a show the co-existence of CV curves in positive and negative potentials, which indicated that the electron transfer in MFCs occurred through both DET and MET.…”

Section: Discussionmentioning

confidence: 99%

“…Microbial activity affects the electrical performance of MFCs mainly through electron transfer 13,14,30 . In MFCs, electron transfer is mainly through two modes: (a) the direct electron transfer (DET) mode, (b) the mediated electron transfer (MET) mode 15 .…”

Section: Discussionmentioning

confidence: 99%

Effect of sulfonamides on the electricity generation by Shewanella putrefaciens in microbial fuel cells

Yang

Jiang

Liu

et al. 2020

Env Prog and Sustain Energy

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Overuse of antibiotics impacts environmental health worldwide. In this paper, the effect on the performance of microbial fuel cells (MFCs) under treatments with sulfonamides (SAs) was investigated. The results showed that when MCFs were treated with sulfamonomethoxine (SMM), the maximum power density was higher (20.95 mW/m 2) than when treated with sulfadiazine (SDZ) (19.79 mW/m 2). The power density of MFCs gradually decreased with increased concentration of SAs. Microbial activity analysis showed that SAs had negative effects on bacterial growth, riboflavin secretion and cell attachment on the MFCs anode. The power density of MFCs at different pH values was in order as follows: pH 6 > pH 7 > pH 8 > pH 5 > pH 9, where the highest was 22.90 mW/m 2 at pH 6. Electrochemical analysis showed that conditions favorable to the protonation of SMM also had a positive effect on the electrical performance of MFCs. These findings could provide the basis for uncovering the mechanism by which SAs affect electricity generation in MFCs, and demonstrates the feasibility for the efficient degradation of SAs and power generation.

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Effect of nanowire conductive transfer on the performance of batch‐microbial fuel cells

Wang

Lan

Ubando

et al. 2021

Intl J of Energy Research

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Microbial fuel cells (MFCs) are a promising technology that uses microorganisms to simultaneously generate bioelectricity while treating wastewater. To further improve the performance of the MFC, it is essential to understand and evaluate the electron transfer mechanism. However, redesigning the electron transfer mechanism of MFCs through an experimental approach is costly and time-consuming. Hence, in this study, a numerical modeling approach is implemented through the Nernst-Monod kinetic equation, which is validated by experimental results. A nanowire conductive transferring pathway is considered between the microorganisms and anode electrodes of a batch-type MFC. Moreover, two types of bacteria are utilized such as the Shewanella oneidensis MR-1 and Shewanella putrefacient with substrate concentrations of 0.5 M sodium lactate. The results have shown that the limiting current density of the MFC from the computational model is 1514 mA m À2 . On the other

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Shewanella putrefaciens CN32 outer membrane cytochromes MtrC and UndA reduce electron shuttles to produce electricity in microbial fuel cells

Cited by 23 publications

References 32 publications

Promoting Shewanella Bidirectional Extracellular Electron Transfer for Bioelectrocatalysis by Electropolymerized Riboflavin Interface on Carbon Electrode

Promoting Shewanella Bidirectional Extracellular Electron Transfer for Bioelectrocatalysis by Electropolymerized Riboflavin Interface on Carbon Electrode

Effect of sulfonamides on the electricity generation by Shewanella putrefaciens in microbial fuel cells

Effect of nanowire conductive transfer on the performance of batch‐microbial fuel cells

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