Shewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines

Satomi, Masataka; Oikawa, Hiroshi; Yano, Yutaka

doi:10.1099/ijs.0.02392-0

Cited by 99 publications

(94 citation statements)

References 48 publications

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“…Table 1. Differential characteristics of strains A3F-7 T and ssthio04PA2-7c (Paramoritella gen. nov.) and related genera Data for reference genera were taken from the following studies: Urakawa et al (1998), Nogi et al (1998b), Nogi & Kato (1999), Benediktsdó ttir et al (2000), Lunder et al (2000), Xu et al (2003) and Kim et al (2008) (Moritella); Bowman et al (1997), Bowman (2005), Nogi et al (1998a), Venkateswaran et al (1999), Leonardo et al (1999), Ivanova et al (2001Ivanova et al ( , 2003Ivanova et al ( , 2004a, Bozal et al (2002), Skerratt et al (2002), Brettar et al (2002, Satomi et al (2003Satomi et al ( , 2006Satomi et al ( , 2007, Yoon et al (2004a, b), Toffin et al (2004), Xu et al (2005), Zhao et al (2005Zhao et al ( , 2006Zhao et al ( , 2007 …”

Section: Description Of Paramoritella Alkaliphila Sp Novmentioning

confidence: 99%

Paramoritella alkaliphila gen. nov., sp. nov., a member of the family Moritellaceae isolated in the Republic of Palau

Hosoya

Suzuki

Adachi

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Two strains of marine, heterotrophic, alkaliphilic bacteria, designated A3F-7 T and ssthio04PA2-7c, were isolated from hard coral and marine sand, respectively, collected in the Republic of Palau. A phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were related to members of the genus Moritella. However, the sequence similarities between the isolates and the type strains of the Moritella species were less than 93 %. The G+C contents of the isolates were around 57 mol%, the major respiratory quinone was Q-8 and the predominant cellular fatty acids were 16 : 1v7c, 16 : 0, 18 : 1v7c and 14 : 0. On the basis of the phylogenetic data, phenotypic characteristics and DNA-DNA hybridization results, strains A3F-7 T and ssthio04PA2-7c represent a novel species of a novel genus, for which the name Paramoritella alkaliphila gen. nov., sp. nov. is proposed. The type strain of Paramoritella alkaliphila is A3F-7 T (5MBIC06429 T 5DSM 19956 T ).The genus Moritella, currently the only known member of the family Moritellaceae (Ivanova et al., 2004b), belongs to the order Alteromonadales (Bowman & McMeekin, 2005) within the class Gammaproteobacteria. Members of this genus are characterized as psychrophilic, halophilic, facultatively anaerobic bacteria (Nogi & Kato, 1999; Lunder et al., 2000; Xu et al., 2003). In this study, we used a polyphasic taxonomic approach to investigate two mesophilic strains that were found to represent a novel genus and species belonging to the family Moritellaceae.Strain A3F-7 T was isolated from hard coral (Favites complanata) collected in August 2003 from Iwayama Bay (GPS location 7 u 19.429 N 134 u 29.689 E) in the Republic of Palau. Strain A3F-7 T was isolated by using marine agar 2216 (Difco) adjusted to pH 11 with Na 2 CO 3 and NaHCO 3 . Strain ssthio04PA2-7c was isolated from marine sand collected in the Republic of Palau (GPS location 7 u 20.089 N 134 u 29.799 E) in September 2004. Strain ssthio04PA2-7c was isolated by using a thio medium consisting of a basal medium (l 21 : 23.5 g NaCl, 4.9 g MgCl 2 , 0.2 g CaCl 2 . 2H 2 O, 0.66 g KCl, 0.096 g KBr, 0.026 g H 3 BO 3 , 0.024 g SrCl 2 , 0.003 g NaF, 1.5 % agar, w/v) to which the following components were added (l 21 ): 2 mg sodium pyruvate, 2 mg sodium succinate, 2 mg sodium ascorbate, 2 mg cysteine hydrochloride, 0.002 % (w/v) yeast extract, 25 % (w/v) sodium thiosulfate, 15 ml NaHCO 3 solution (84 g NaHCO 3 l 21 ); 1 ml vitamin solution (20 nM each of biotin, 4-aminobenzoic acid, pantothenate, pyridoxine, nicotinamide, thiamine, lipoic acid, folic acid, vitamin B 12 and riboflavin), 1 ml trace element solution of DSMZ medium 69 (DSMZ, 1993), 1 ml selenite-tungstate solution (0.01 mM Na 2 SeO 3 and 0.01 mM Na 2 WO 4 . 2H 2 O) and 1 ml vitamin B 12 solution (150 mg cyanocobalamin l 21 ). Each isolate was then cultured and maintained on 1/5-diluted marine agar (800 ml filtered seawater, 200 ml distilled water, 7.48 g marine broth and 15 g agar).Nucleotide sequences of the 16S rRNA gene amplified by using primers 27F an...

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Section: Description Of Paramoritella Alkaliphila Sp Novmentioning

confidence: 99%

Paramoritella alkaliphila gen. nov., sp. nov., a member of the family Moritellaceae isolated in the Republic of Palau

Hosoya

Suzuki

Adachi

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…2). The sequence similarity values required to separate species on the basis of the gyrB gene vary according to the genus (Venkateswaran et al, 1999;Satomi et al, 2002Satomi et al, , 2003Satomi et al, , 2004. Additional reputable genetic analyses are therefore necessary to confirm the novelty of these isolates.…”

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confidence: 99%

Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces

Satomi

Duc

Venkateswaran

2006

International Journal of Systematic and Evolutionary Microbiology

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178

116

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Thirteen strains of a novel spore-forming, Gram-positive, mesophilic heterotrophic bacterium were isolated from spacecraft surfaces (Mars Odyssey Orbiter) and assembly-facility surfaces at the Jet Propulsion Laboratory in California and the Kennedy Space Center in Florida. Phylogenetic analysis of 16S rRNA gene sequences has placed these novel isolates within the genus Bacillus, the greatest sequence similarity (99?9 %) being found with Bacillus pumilus. However, these isolates share a mere 91?2 % gyrB sequence similarity with Bacillus pumilus, rendering their 16S rRNA gene-derived relatedness suspect. Furthermore, DNA-DNA hybridization showed only 54-66 % DNA relatedness between the novel isolates and strains of B. pumilus. rep-PCR fingerprinting and previously reported matrix-assisted laser desorption/ionization time-of-flight mass spectrometry protein profiling clearly distinguished these isolates from B. pumilus. Phenotypic analyses also showed some differentiation between the two genotypic groups, although the fatty acid compositions were almost identical. The polyphasic taxonomic studies revealed distinct clustering of the tested strains into two distinct species. On the basis of phenotypic characteristics and the results of phylogenetic analyses of 16S rRNA and gyrB gene sequences, repetitive element primer-PCR fingerprinting and DNA-DNA hybridization, the 13 isolates represent a novel species of the genus Bacillus, for which the name Bacillus safensis sp. nov. is proposed. The type strain is FO-36b T (=ATCC BAA-1126 T =NBRC 100820 T ).Spacecraft and associated clean-room assembly-facility surfaces harbour an extremely low biomass (La Duc et al., 2003;Venkateswaran et al., 2001), because of stringent maintenance. However, colonization by micro-organisms specifically adapted to such facility conditions, especially those yet to be cultured and/or characterized, is of major concern to those commissioning modern-day space-related experimentation. The search for extraterrestrial life will rely heavily on validated cleaning and bioreduction strategies to ensure that terrestrial microbial contamination does not compromise the scientific integrity of such missions. It is crucial both to minimize and eradicate such microbial contaminants and to identify and characterize the recurring, prevalent micro-organisms associated with the surfaces of spacecraft and associated environments.Studies have repeatedly shown that extremely resilient, spore-forming members of the genus Bacillus are the most strongly represented micro-organisms in samples collected from spacecraft and facility surfaces (La Duc et al., 2003; Puleo et al., 1977). The extremely oligotrophic, lowhumidity, temperature-controlled conditions of spacecraftassembly facilities appear to select for micro-organisms able to withstand such unfavourable surroundings. During monitoring of the microbial diversity of spacecraftassociated environments over a period of 5 years (1999)(2000)(2001)(2002)(2003)(2004), Bacillus pumilus was found to be the second most domi...

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“…shewanella.org). Some Shewanella strains are also able to degrade pollutants such as chlorinated solvents (Petrovskis et al, 1994), petroleum (Semple & Westlake, 1987) and RDX (1,3,5-trinitroperhydro-1,3,5-triazine) (Zhao et al, 2004), some can produce polyunsaturated fatty acids (Bowman et al, 1997;Russell & Nichols, 1999;Satomi et al, 2003) and some are able to grow under extreme conditions (Bozal et al, 2002;Kato et al, 1998;Nogi et al, 1998;Stapleton et al, 2005).…”

mentioning

confidence: 99%

“…While many Shewanella strains remain uncharacterized, there are 32 recognized Shewanella species: the latter were isolated from a variety of sources, primarily aquatic environments and sediments (Bowman et al, 1997;Bozal et al, 2002;Brettar et al, 2002;Coyne et al, 1989;Ivanova et al, 2001Ivanova et al, , 2003aIvanova et al, , b, 2004aLeonardo et al, 1999;Makemson et al, 1997;Nogi et al, 1998;Nozue et al, 1992;Satomi et al, 2003Satomi et al, , 2006Skerratt et al, 2002;Toffin et al, 2004;Venkateswaran et al, 1998Venkateswaran et al, , 1999Xu et al, 2005;Yoon et al, 2004a, b;Zhao et al, 2005Zhao et al, , 2006Ziemke et al, 1998). The bacteria of this genus have attracted great attention because of their diverse respiratory capacities, illustrated by their ability to utilize a wide range of terminal electron acceptors, including oxygen, nitrate, metals and sulfur compounds (Kostka et al, 1996;Myers & Nealson, 1988;Venkateswaran et al, 1999; http://www.…”

mentioning

confidence: 99%

Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean

Gao

Obraztova

Stewart

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

109

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A novel marine bacterial strain, PV-4 T , isolated from a microbial mat located at a hydrothermal vent of Loihi Seamount in the Pacific Ocean, has been characterized. This micro-organism is orangey in colour, Gram-negative, polarly flagellated, facultatively anaerobic and psychrotolerant (temperature range, 0-42 6C). No growth was observed with nitrate, nitrite, DMSO or thiosulfate as the electron acceptor and lactate as the electron donor. The major fatty acid detected in strain PV-4 T was iso-C 15 : 0 . Strain PV-4 T had ubiquinones consisting mainly of Q-7 and Q-8, and possessed menaquinone MK-7. The DNA G+C content of the strain was 53?8 mol% and the genome size was about 4?5 Mbp. Phylogenetic analyses based on 16S rRNA gene sequences placed PV-4 T within the genus Shewanella. PV-4 T exhibited 16S rRNA gene sequence similarity levels of 99?6 and 97?5 %, respectively, with respect to the type strains of Shewanella aquimarina and Shewanella marisflavi. DNA from strain PV-4 T showed low mean levels of relatedness to the DNAs of S. aquimarina (50?5 %) and S. marisflavi (8?5 %). On the basis of phylogenetic and phenotypic characteristics, the bacterium was classified in the genus Shewanella within a distinct novel species, for which the name Shewanella loihica sp. nov. is proposed. The type strain is PV-4 T (=ATCC BAA-1088 T =DSM 17748 T ).The genus Shewanella consists of rod-shaped, Gramnegative, facultatively anaerobic, readily cultivated gammaproteobacteria (Gauthier et al., 1995;MacDonell & Colwell, 1985;Venkateswaran et al., 1999). While many Shewanella strains remain uncharacterized, there are 32 recognized Shewanella species: the latter were isolated from a variety of sources, primarily aquatic environments and sediments (Bowman et al., 1997;Bozal et al., 2002;Brettar et al., 2002;Coyne et al., 1989;Ivanova et al., 2001 Ivanova et al., , 2003a Ivanova et al., , b, 2004aLeonardo et al., 1999;Makemson et al., 1997;Nogi et al., 1998;Nozue et al., 1992;Satomi et al., 2003Satomi et al., , 2006Skerratt et al., 2002; Toffin et al., 2004;Venkateswaran et al., 1998Venkateswaran et al., , 1999Xu et al., 2005; Yoon et al., 2004a, b;Zhao et al., 2005Zhao et al., , 2006Ziemke et al., 1998). The bacteria of this genus have attracted great attention because of their diverse respiratory capacities, illustrated by their ability to utilize a wide range of terminal electron acceptors, including oxygen, nitrate, metals and sulfur compounds (Kostka et al., 1996;Myers & Nealson, 1988;Venkateswaran et al., 1999; http://www. shewanella.org). Some Shewanella strains are also able to degrade pollutants such as chlorinated solvents (Petrovskis et al., 1994), petroleum (Semple & Westlake, 1987) and RDX (1,3,5-trinitroperhydro-1,3,5-triazine) (Zhao et al., 2004), some can produce polyunsaturated fatty acids (Bowman et al., 1997;Russell & Nichols, 1999;Satomi et al., 2003) and some are able to grow under extreme conditions (Bozal et al., 2002;Kato et al., 1998;Nogi et al., 1998;Stapleton et al., 2005).In a previous study, several She...

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Shewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines

Cited by 99 publications

References 48 publications

Paramoritella alkaliphila gen. nov., sp. nov., a member of the family Moritellaceae isolated in the Republic of Palau

Paramoritella alkaliphila gen. nov., sp. nov., a member of the family Moritellaceae isolated in the Republic of Palau

Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces

Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean

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