Shenqi Fuzheng Injection impairs bile duct ligation-induced cholestatic liver injury in vivo

Abstract

Background and aim: The aim of the present study sought to determine the protective function of Shenqi Fuzheng Injection (SFI) in cholestatic liver injury.Methods: Cholestatic liver injury was induced in a 7-day bile duct-ligated (BDL) rat model. Rats were divided into three groups that were comprised of: (1) Sham; (2) BDL model; and (3) SFI treatment. The sham and BDL groups were treated with an appropriate volume of 0.9% sodium chloride as the vehicle, and the SFI group was administered SFI at a dose of 20 ml/kg/day, via tail vein injection.Results: SFI significantly (all at P<0.01) decreased the levels of serum aspartate aminotransferase and alanine aminotransferase as compared with the BDL group, which was associated with reduced severity of inflammatory cell infiltration and hepatic damage. Moreover, SFI significantly decreased the levels of hepatic interleukin-6 (P<0.01), tumor necrosis factor-α (P=0.041), and malondialdehyde (P=0.026), and significantly increased the levels of total superoxide dismutase (P<0.01), and the GSH/GSSG ratio (P=0.041) in the liver. Western blot analysis showed that SFI increased PPAR-γ expression; however, SFI treatment decreased cyclooxygenase-2 (COX-2) expression and the phosphorylation of NF-κBp65.Conclusions: These data demonstrated that SFI attenuated both inflammation and oxidative stress, and disrupted cholestatic liver injury. The involved mechanism was dependent, at least in part, on regulating PPAR-γ, COX-2, and NF-κBp65 expression.

Lipolysis-Stimulated Lipoprotein Receptor Impairs Hepatocellular Carcinoma and Inhibits the Oncogenic Activity of YAP1 via PPPY Motif

“…After 14 days, the cells were stained with 0.2% crystal violet (Damao, Tianjin, China) and the number of colonies, consisting of 50 or more cells, were determined with the help of a Leica DMI4000B microscopy (Leica, Mannheim, Germany). To evaluate the effect of LSR in vivo , six-week-old female BALB/c (CAnN.Cg-Foxn1 nu /Crl) nude mice were purchased from Charles River (Wilmington, MA, USA) and bred in the central animal facility of the Dalian Medical University as previously described ( 26 ). 2 × 10 6 HCC cells or the identical number of LSR knockdown cells were resuspended in 100 µL phosphate-buffered saline and subcutaneously injected into the right flank of the BALB/c nude mice.…”

Anti-inflammatory, anti-oxidative stress and novel therapeutic targets for cholestatic liver injury

“…Drugs Celastrol (27) Andrographolide (28) Rosuvastatin (29) Shenqi Fuzheng Injection (30) Peroxiredoxin 4 (31) 9-cis-retinoic acid (32) Schisandrol B (33) Glechoma hederacea (34) Chicken bile powder (35) Auraptene (36) SRT1720 (37) Sweroside (38) Chlorogenic acid (39) Vitamin C (40) Thymoquinone (41) Cilostazol (42) Quercetin (43) Guava Pulp (44) Serotonin (45) Docosahexaenoic acid (46) Cyclopamine (47) Resveratrol (48) Caffeic acid phenethyl ester (49) Tetrathiomolybdate (50) Aminoguanidine ( IL-1alpha, TNF-alpha a novel membrane-bound bile acid receptor expressed in many types of cells, which plays an important role in regulating energy homeostasis and glucose metabolism. In vitro experiments showed that TGR5 significantly inhibited macrophage function (66).…”