Shellmycin A–D, Novel Bioactive Tetrahydroanthra-γ-Pyrone Antibiotics from Marine Streptomyces sp. Shell-016

Han, Yong Chol; Wang, Yan; Yang, Yuehan; Chen, Haotong

doi:10.3390/md18010058

Cited by 14 publications

(8 citation statements)

References 34 publications

(43 reference statements)

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“…Four compounds, shellmicin A-D isolated from the Streptomyces sp. shell-016 were described as cytotoxic agents against five human tumor cell lines: non-small cell lung cancer (H1299, ATCC CRL-5803), malignant melanoma (A375, ATCC CRL- 1619), hepatocellular carcinoma (HepG2, ATCC HB-8065), colorectal adenocarcinoma (HT29 ATCC HTB-38), and breast cancer (HCC1937, ATCC CRL-2336) [ 55 ]. Moreover, the compounds Shellmicin A, B, and D showed greater cytotoxicity (IC 50 ranging from 0.69 μM to 3.11 μM at 72 h) than Shellmicin C. Interestingly, shellmicin C and D are a pair of stereoisomers, and their biological activity differ considerably.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru

et al. 2021

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Extreme environments Morrope and Bayovar Salt lagoons, several ecosystems and microhabitats remain unexplored, and little is known about the diversity of Actinobacteria. We suggest that the endemic bacteria present in this extreme environment is a source of active molecules with anticancer, antimicrobial, and antiparasitic properties. Using phenotypic and genotypic characterization techniques, including 16S rRNA sequencing, we identified these bacteria as members of the genera Streptomyces, Pseudonocardia, Staphylococcus, Bacillus, and Pseudomonas. Actinobacteria strains were found predominantly. Phylogenetic analysis revealed 13 Actinobacteria clusters of Streptomyces, the main genus. Three Streptomycetes, strains MW562814, MW562805, and MW562807 showed antiproliferative activities against three tumor cell lines: U251 glioma, MCF7 breast, and NCI-H460 lung (non-small cell type); and antibacterial activity against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 10536, and the multidrug resistant Acinetobacter baumannii AC-972. The antiproliferative activities (measured as total growth inhibition [TGI]) of Streptomyces sp. MW562807 were 0.57 μg/mL, for 0.61 μg/mL, and 0.80 μg/mL for glioma, lung non-small cell type, and breast cancer cell lines, respectively; the methanolic fraction of the crude extract showed a better antiproliferative activity and could inhibit the growth of (U251 (TGI = 38.3 μg/mL), OVCAR-03 (TGI = 62.1 μg/mL), and K562 (TGI = 81.5 μg/mL)) of nine tumor cells types and one nontumor cell type. Extreme enviroments, such as the Morrope and Bayovar Salt saloons are promising sources of new bacteria, whose compounds may be useful for treating various infectious diseases or even some types of cancer.

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Section: Resultsmentioning

confidence: 99%

Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru

et al. 2021

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“…Four compounds called shellmicin A-D isolated from the Streptomyces sp. shell-016 were described by (Yong Han et al 2020) and studied concerning cytotoxicity against five human tumor cells lines: non-small cell lung cancer (H1299, ATCC CRL-5803), malignant melanoma (A375, ATCC CRL-1619), hepatocellular carcinoma (HepG2, ATCC HB-8065), colorectal adenocarcinoma (HT29 ATCC HTB-38) and breast cancer (HCC1937, ATCC CRL-2336). The study indicated that the compounds Shellmicin A, B and D showed greater cytotoxicity than Shellmicin C compound, with an IC 50 ranging from 0.69 µM to 3.11 µM at 72 h. An interesting fact was that shellmicin C and D are a pair of stereoisomers and their biological activity was significantly different.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Antimicrobial and Antiproliferative Activities of Actinobacteria Isolated From the Saline Lagoons of Northwest Peru

Clavo

Quiñones

Hernandez

et al. 2020

Preprint

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The unexplored saline lagoons of the north of Peru harbor a rich microbiome, due to reported studies of different extreme environments around the world. In these regions, there are several ecosystems and microhabitats not yet explored, and little is known about the diversity of actinobacteria and other microorganisms. We suggest that the endemic bacteria present in this extreme environment could be source of active molecules with anticancer, antimicrobial, antiparasitic properties. Using phenotypic and genotypic characterization techniques including the 16S rRNA were identified into the genera Streptomyces 39 (78%), Pseudonocardia 3 (6%), Staphylococcus 4 (8%), Bacillus 2 (4%), and Pseudomonas 2 (4%). All isolated bacteria for the genotypic data were preliminarily identified. Actinobacteria strains were found dominantly in both sites (Lagoon1-3 = 16 isolates and lagoon 4 = 12 isolates). Phylogenetic analysis revealed that 28 isolates were exclusively affiliated to eleven different clusters of Actinobacteria of the major genus Streptomyces. Three Streptomyces sp. strains M-92, B-146, and B-81, were tested for antibacterial and antiproliferative activities. The results showed antiproliferative activities against three tumor cell lines, U251 glioma; MCF7 breast; NCI-H460 lung non-small type of cells, and the antibacterial activity to Staphylococcus aureus ATCC 6538, E. coli ATCC 10536, and Acinetobacter baumanni AC-972 which is resistant to multiple drugs. The promising results belong to Streptomyces sp. B-81 strain in the R2A medium using a doxorubicin with control positive, the best result was from the latter (TGI = 0,57 μg/mL) for glioma; NCI-H460 lung of type non-small cells (TGI = 0,61 μg/mL), and breast cancer (TGI =0,80 μg/mL), this strain was selected to be fractionated because it had better antiproliferative and antibacterial activity, and its fractions were evaluated concerning antiproliferative activity against nine types of tumor cells and one non-tumor. The methanolic fraction showed a better result in the antiproliferative activity and was able to inhibit U251 (glioma) (TGI = 38.3 μg/mL), OVCAR-03 (ovary) (TGI = 62.1 μg/mL), and K562 (leukemia) (TGI = 81.5 μg/mL). The methanol 50% - acetate 50% fraction (Fraction 4) inhibited U251 (glioma) (TGI = 73.5 μg/mL) and UACC-62 (melanoma) (TGI = 89.4 μg/mL). Moreover, the UHPLC-MS/MS data and molecular networking of Streptomyces sp. B-81 isolate extract revealed the production cholic acid, Lobophorin A, Lobophorin B, Lobophorin E, Lobophorin K and compound 6. Extremophilic environments such as the Mórrope and Bayovar Salt Flats are promising sources of new bacteria with promising pharmaceutical potential; These compounds could be useful to treat various infectious diseases or even some type of cancer.

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“…Another example is tetrahydroanthra-γ-pyrone from marine Streptomyces sp. (isolated from Binzhou shell island), which presented activity against Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis with a minimum inhibitory concentration (MIC) from 3 to 46 μg/mL [26].…”

Section: Antibioticsmentioning

confidence: 99%

Microorganisms as Alternative Sources of New Natural Products

Cabello¹

2021

Bioactive Compounds in Nutraceutical and Functional Food for Good Human Health

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Microbial natural products have become important over the last decades due to the ability of bacteria and fungi to subsist in different habitats such as marine and extreme environments. Microorganisms are able to synthesize new compounds with diverse therapeutic activity equal to or better than the activity of compounds already known, thus being promising for the treatment of different diseases such as cancer or the solution to health problems such as antibiotic resistance. The production of microbial natural compounds can be improved by modifying culture media, growing conditions, amplifying gene expression or by co-cultivation techniques, which are the major challenges in the industrial production of such compounds.

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Shellmycin A–D, Novel Bioactive Tetrahydroanthra-γ-Pyrone Antibiotics from Marine Streptomyces sp. Shell-016

Cited by 14 publications

References 34 publications

Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru

Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru

Evaluation of Antimicrobial and Antiproliferative Activities of Actinobacteria Isolated From the Saline Lagoons of Northwest Peru

Microorganisms as Alternative Sources of New Natural Products

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