Shell Extracts from the Marine Bivalve Pecten maximus Regulate the Synthesis of Extracellular Matrix in Primary Cultured Human Skin Fibroblasts

Abstract

Mollusc shells are composed of more than 95% calcium carbonate and less than 5% of an organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. Previous studies have elucidated the biological activities of the shell matrices from bivalve molluscs on skin, especially on the expression of the extracellular matrix components of fibroblasts. In this work, we have investigated the potential biological activities of shell matrix components extracted from the shell of the scallop Pecten maximus on human fibroblasts in primary culture. Firstly, we demonstrated that shell matrix components had different effects on general cellular activities. Secondly, we have shown that the shell matrix components stimulate the synthesis of type I and III collagens, as well as that of sulphated GAGs. The increased expression of type I collagen is likely mediated by the recruitment of transactivating factors (Sp1, Sp3 and human c-Krox) in the −112/−61 bp COL1A1 promoter region. Finally, contrarily to what was obtained in previous works, we demonstrated that the scallop shell extracts have only a small effect on cell migration during in vitro wound tests and have no effect on cell proliferation. Thus, our research emphasizes the potential use of shell matrix of Pecten maximus for dermo-cosmetic applications.

Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro

“…(e.g., Berland et al 2005;Perreira-Mouriès et al 2002a;Rousseau et al 2003), while the few additional works based on non-nacreous bivalves took the scallop as a model (e.g. Liu et al 2002Liu et al , 2006Torita et al 2007;Latire et al 2014). In the present study, we have expanded the scope of investigation by using another nacreous bivalve, the blue mussel Mytilus edulis, but also one non-nacreous species, the Pacific oyster Crassostrea gigas.…”

“…For example, extracts from the giant Ezo scallop Patinopecten yessoensis shell enhance the turnover rate of the epidermal layer and increase the recovery efficiency of the dorsal skin in UV-injured rats (Liu et al 2006). Recently, Latire et al (2014) have demonstrated that shell matrix components from the great scallop Pecten maximus regulate the synthesis of the extracellular matrix in primary-cultured human skin fibroblasts. More precisely, shell matrix components stimulate the synthesis of type I and III collagens, as well as that of sulphated GAGs.…”

Shells from aquaculture: a valuable biomaterial, not a nuisance waste product

“…()) or in functional cosmetics (Latire et al . ), will not provide solutions to large‐scale shell waste issues, which is the focus of this article. Previous articles have reviewed aquaculture and shell waste valorisation from a more generalised perspective without specific considerations for scalability or sustainability (Ferraro et al .…”

“…There is also a plethora of potential small-scale shell valorisation techniques. Although such applications are interesting in the discussion of innovation in waste reuse, these techniques, such as the use of shell powder in biomedical techniques (as highlighted by Green et al (2015)) or in functional cosmetics (Latire et al 2014), will not provide solutions to large-scale shell waste issues, which is the focus of this article. Previous articles have reviewed aquaculture and shell waste valorisation from a more generalised perspective without specific considerations for scalability or sustainability (Ferraro et al 2010;Yao et al 2014).…”

Marine Mollusks: Food with Benefits

“…Many molluscan species find use in traditional medication in different parts of the world (Chakraborty, Hsu, Wen, & Lin, ). Molluscan shells (Latire et al., ), soft tissue, basal parts, mucilage, and even entire organisms (Kehinde, Mariam, Adebimpe, & Blessing, ) have been traditionally used as medication for curing gastrointestinal ailments, cancer, inflammations, dotage, and some other diseases. The customary practitioners, though, utilized mollusks on the basis of information carried through generations built upon repeated trial and error.…”