Sheet-beam geometry for in vivo fluorescent x-ray computed tomography: proof-of-concept experiment in molecular imaging

Huo, Qingkai; Yuasa, Tetsuya; Akatsuka, Takao; Takeda, Takeshi; Wu, Jin; Thet-Thet-Lwin,; Hyodo, Kazuyuki; Dilmanian, F.A.

doi:10.1364/ol.33.002494

Cited by 32 publications

(16 citation statements)

References 11 publications

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“…Therefore, both the in-vivo and ex-vivo FXCT images of biomedical samples reported thus far have been single-cross-sectional. For 3-D imaging, a 2-D projection batch acquisition scheme is indispensable 19, 20 .…”

Section: Introductionmentioning

confidence: 99%

Multi-pinhole fluorescent x-ray computed tomography for molecular imaging

Sasaya

Sunaguchi

Hyodo

et al. 2017

Sci Rep

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We propose a multi-pinhole fluorescent x-ray computed tomography (mp-FXCT) technique for preclinical molecular imaging that can provide the complete data necessary to produce 3-D tomographic images during anaesthesia. In this method, multiple projections are simultaneously acquired through a multi-pinhole collimator with a 2-D detector and full-field volumetric beam to accelerate the data acquisition process and enhance the signal-to-noise ratios of the projections. We constructed a 15-pinhole mp-FXCT imaging system at beamline ARNE-7A at KEK and performed preliminary experiments to investigate its imaging properties using physical phantoms and a non-radioactive I imaging agent. The mp-FXCT system could detect an I concentration of 0.038 mg/ml, the minimum required for in-vivo imaging, at a spatial resolution of about 0.3 mm during a data acquisition time of 90 min, which is less than the time for which anaesthesia is effective and suggests that preclinical molecular imaging is feasible with mp-FXCT.

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Section: Introductionmentioning

confidence: 99%

Multi-pinhole fluorescent x-ray computed tomography for molecular imaging

Sasaya

Sunaguchi

Hyodo

et al. 2017

Sci Rep

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“…Fluorescent x-ray computed tomography (FXCT), which combines x-ray fluorescence analysis and tomographic reconstruction algorithms, delineates the spatial distribution of imaging agents within samples with high sensitivity, reproducible and quantifiable measures, and at high spatial resolutions, in a non-destructive and non-invasive manner [1][2][3]. FXCT has been a vital tool in both material and biomedical sciences [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…Unfortunately, it takes an enormous amount of acquisition time to acquire a 3-dimensional tomographic image as the pencil beam method collects sequential data. In order to overcome this difficulty, a parallel data acquisition scheme has been proposed using an incident-sheet beam using a linear detectors array [3]. We demonstrated the efficacy using a preliminary simulated system.…”

Section: Introductionmentioning

confidence: 99%

The feasibility study on 3-dimensional fluorescent x-ray computed tomography using the pinhole effect for biomedical applications

Sunaguchi

Yuasa

Hyodo³

et al. 2013

2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

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We propose a 3-dimensional fluorescent x-ray computed tomography (CT) pinhole collimator, aimed at providing molecular imaging with quantifiable measures and sub-millimeter spatial resolution. In this study, we demonstrate the feasibility of this concept and investigate imaging properties such as spatial resolution, contrast resolution and quantifiable measures, by imaging physical phantoms using a preliminary imaging system developed with monochromatic synchrotron x rays constructed at the BLNE-7A experimental line at KEK, Japan.

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“…[15][16][17][18][19][20] The pencil beam/single collimated beam from polychromatic x-ray tubes [21][22][23][24][25][26][27] is then used to improve the feasibility of using XFCT; however, this technique requires a long scanning time, e.g., 279 min 28 and 180 h. 29 One important reason for the low efficiency is that using a pencil beam setup requires the beam source to be shifted point by point while measuring a single slice of projection data. Similar processes of shifting on detectors are also required for a fan-beam setup with a single pixel detector.…”

mentioning

confidence: 99%

Full-field fan-beam x-ray fluorescence computed tomography with a conventional x-ray tube and photon-counting detectors for fast nanoparticle bioimaging

Zhang

et al. 2017

Opt. Eng

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, "Full-field fan-beam x-ray fluorescence computed tomography with a conventional x-ray tube and photon-counting detectors for fast nanoparticle bioimaging," Opt. Eng. 56(4), 043106 (2017), doi: 10.1117/1.OE.56.4.043106. Abstract. X-ray fluorescence computed tomography (XFCT) was performed on a high-intensity synchrotron radiation source or a pencil beam with a long exposure time due to the low emission and detection efficiency of x-ray fluorescence photons. For the first time, the feasibility and experimental results of a full-field fan-beam XFCT with a photon-counting detector array are presented. This full-field fan-beam XFCT consists of a conventional low-intensity x-ray tube, an energy-sensitive photon-counting detector array, and a tungsten pinhole collimator. A phantom containing gadolinium solution (K α , 42.74 keV) was scanned for 30 min using a polychromatic x-ray fan beam with a third-generation computed tomography (CT) geometry. After scattering and attenuation corrections, experimental results showed that XFCT had better accuracy and performance than spectral CT. Full-field XFCT is a promising modality for biomedical imaging of exogenous molecular probes containing nanoparticles of high atomic number.

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Sheet-beam geometry for in vivo fluorescent x-ray computed tomography: proof-of-concept experiment in molecular imaging

Cited by 32 publications

References 11 publications

Multi-pinhole fluorescent x-ray computed tomography for molecular imaging

Multi-pinhole fluorescent x-ray computed tomography for molecular imaging

The feasibility study on 3-dimensional fluorescent x-ray computed tomography using the pinhole effect for biomedical applications

Full-field fan-beam x-ray fluorescence computed tomography with a conventional x-ray tube and photon-counting detectors for fast nanoparticle bioimaging

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