Sheep toxoplasmosis diagnose by ELISA and IFAT

Abstract

Toxoplasmosis in sheep herds is a major cause of abortion, causes great economic impacts and sheep are a potential transmitters of the disease, either by meat consumption or contaminated dairy products. The diagnosis of toxoplasmosis is done by serological and molecular techniques. The reaction of indirect immunofluorescence assay (IFA) and enzyme immunoassay (ELISA) are most commonly used diagnostic methods. The seroprevalence of toxoplasmosis in sheep herd IZ was 16.86% (ELISA) and 21.51% (IFAT), the results between the two tests showed sensitivity of 77.70%, specificity of 99.25%, positive predictive value of 96.55%, negative predictive value of 94.36% and Kappa 0.83 (CI 1.0 to 0.796). Kappa index showed almost perfect agreement, reinforcing the choice of ELISA as a screening method. There was no significant statistical difference (P>0.05) of 95% in the Fisher test between races and ages of the sheep.